关键词: 溃疡病, 大黄鱼, 组织病理, 超微病理

Abstract:

The skeletal muscle, gill, kidney, spleen and intestine of Pseudosciaena crocea infected with ulcer disease were pathologically analyzed by tissue slice and ultramicrocut electron microscopy techniques. The histopathology showed patho-logical changes of ulcer disease were degenerative inflammation. The main manifestations were the broken myofilaments and inflammatory infiltration in skeletal muscle, and swelling and fusion in the gill. Denaturalization and necrosis emerged in the cells of gill, and the vacuolations, in the hepatic parenchymal cells. The connective tissues showed abnormity, so did the renal tubular epithelial cells. In addition, diffused hemorrhage was found in the kidney, and inflammation was seen in the spleen and intestine. The findings in ultrastructure showed that the myofilaments of skeletal muscle were disturbed and broken, and that the swelling and rupture appeared in the mitochondria as well as the mitochondria in the liver. The liver showed fatty degeneration of hepatocyte and necrosise evidently. The structure and nucleolus of kidney changed; the spleen cells formed fibrosis, and the structure of intestinal walls was seriously destroyed. The results indicated that pathological change widely occurred in ulcer dis-ease of Pseudosciaena crocea tissue cells and caused serious damage to body tissue and the function of liver, spleen, kidney and other major organs. The infected fish lost the normal physiological metabolism, and its feeding ability was gradually diminished, which eventually led to its death. Finally, some suggestions are given for preventing ulcer disease of Pseudosciaena crocea.

Key words: ulcer disease, Pseudosciaena crocea, histopathology, ultrastructure