不同氮磷比对中肋骨条藻和威氏海链藻生长特性的影响

doi:10.11978/j.issn.1009-5470.2010.06.092

热带海洋学报 ›› 2010, Vol. 29 ›› Issue (6): 92-97.doi: 10.11978/j.issn.1009-5470.2010.06.092cstr: 32234.14.j.issn.1009-5470.2010.06.092

不同氮磷比对中肋骨条藻和威氏海链藻生长特性的影响

刘皓1, 2, 高永利1, 殷克东1, 袁翔城1, 徐杰3, HARRISON Paul J 3

1. 中国科学院南海海洋研究所热带海洋环境动力学实验室, 广东广州 510301; 2. 中国科学院研究生院, 北京 100049; 3. 香港科技大学, 海岸海洋环境科学系, 香港

收稿日期:2009-03-24 修回日期:2009-08-26 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 殷克东。
作者简介:刘皓(1981—), 男, 吉林省长春市人, 硕士研究生, 主要从事藻类生态及营养盐限制、细菌与藻类之间的作用方面的研究。E-mail: super-liu@hotmail.com
基金资助:
中国科学院南海海洋所研究所前沿领域青年项目(SQ200815); 中国科学院、国家外国专家局创新团队国际合作伙伴计划

Effects of N to P ratio on the growth of two red tide diatom Skeletonema costatum and Thalassiosira weissflogii

LIU Hao1,2, Gao Yong-li1, YIN Ke-dong1, YUAN Xiang-cheng1, XU Jie3, HARRISON Paul J 3

1. Key Laboratory of Tropical Marine Environmental Dynamics, South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100049, China; 3. Atmospheric, Marine and Coastal Environmental Program, Hong Kong University of Science and Technology, Hong Kong

Received:2009-03-24 Revised:2009-08-26 Online:2010-12-15 Published:2010-12-15
Contact: 殷克东。
About author:刘皓(1981—), 男, 吉林省长春市人, 硕士研究生, 主要从事藻类生态及营养盐限制、细菌与藻类之间的作用方面的研究。E-mail: super-liu@hotmail.com
Supported by:
中国科学院南海海洋所研究所前沿领域青年项目(SQ200815); 中国科学院、国家外国专家局创新团队国际合作伙伴计划

摘要/Abstract

摘要：

实验室条件下用不同氮磷摩尔比(4︰1, 16︰1, 64︰1)的培养液培养中肋骨条藻Skeletonema costatum和威氏海链藻Thalassiosira weissflogii, 对它们的比生长率、细胞状态、细胞对外界氮磷营养元素的吸收和细胞内氮磷比的变化进行了研究。结果表明, 氮磷比显著影响两种硅藻的生长和生理状态, 氮浓度对细胞生长的影响更大。N限制组(N︰P=4︰1)的比生长率、细胞数量和叶绿素a含量明显低于正常条件和P限制组(N︰P=64︰1); 威氏海链藻生长对N的变化比中肋骨条藻更为敏感, 吸收外界无机氮的速率更快。营养盐充足的情况下, 水体中藻细胞的氮磷比变化会较小, 但由于“奢侈消费”现象的存在, 在出现营养盐限制时, 细胞的氮磷比组成会跟随环境的氮磷比改变, 在氮限制的条件下, 细胞的氮磷比会相应减少, 而相反在磷限制的条件下, 细胞的氮磷比会明显增加。

关键词: 氮磷比, 中肋骨条藻Skeletonema costatum, 威氏海链藻Thalassiosira weissflogii, 氮限制, 磷限制

Abstract:

The growth character and ecophysiology of Skeletonema costatum and Thalassiosira weissflogii were studied in culture medium of different ambient ratios of N to P (4︰1, 16︰1, 64︰1). Bacth cultures of these two phytoplankton species were conducted to examine their growth rates, Chl-a, DIN︰P (dissolved inorganic nitrogen and phosphorus) and PON︰P (particular organic nitrogen and phosphorus) during incubation periods. The results showed that the N︰P ratio could signifi-cantly influence the growth of Skeletonema costatum and Thalassiosira weissflogii. The ratio of N to P could evidently influ-ence these two diatom species. The growth rate, cell numbers and Chl-a of these two species were obviously lower with N-limitation (N︰P=4︰1) than those with P-limitation (N︰P=64︰1). The results suggested that T. weissflogii was more sensitive to N-limitation than S. costatum. Although cultured in different N︰P ratios medium, the N︰P composition of these two algal species hardly changed with the medium N︰P ratio. Due to the luxury consumption, the cells could still take up excessive nutrients for living when other nutrients were depleted by phytoplankton, which resulted in different N︰P ratios of phytoplankton. N-limited medium reduced the N︰P ratio of the algal cells, while P-limited medium increased the N︰P ratio of the phytoplankton cells significantly.

Key words: N︰P ratio, Skeletonema costatum, Thalassiosira weissflogii, Nitrogen-limitation, Phosphorus-limitation

刘皓,高永利,殷克东,袁翔城,徐杰,HARRISON Paul J . 不同氮磷比对中肋骨条藻和威氏海链藻生长特性的影响[J]. 热带海洋学报, 2010, 29(6): 92-97.

LIU Hao,Gao Yong-li,YIN Ke-dong,YUAN Xiang-cheng,XU Jie,HARRISON Pau. Effects of N to P ratio on the growth of two red tide diatom Skeletonema costatum and Thalassiosira weissflogii[J]. Journal of Tropical Oceanography, 2010, 29(6): 92-97.

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不同氮磷比对中肋骨条藻和威氏海链藻生长特性的影响

Effects of N to P ratio on the growth of two red tide diatom Skeletonema costatum and Thalassiosira weissflogii

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