川纹笛鲷染色体核型、银染和C-带

doi:10.11978/j.issn.1009-5470.2011.01.091

热带海洋学报 ›› 2011, Vol. 30 ›› Issue (1): 91-95.doi: 10.11978/j.issn.1009-5470.2011.01.091cstr: 32234.14.j.issn.1009-5470.2011.01.091

川纹笛鲷染色体核型、银染和C-带

郭明兰1, 游欣欣2, 苏永全2, 丁少雄2, 王军2

1. 中国科学院南海海洋研究所海洋生物资源可持续利用重点实验室, 广东广州 510301; 2. 厦门大学海洋与环境学院, 福建厦门 361005

收稿日期:2009-05-12 修回日期:2010-06-22 出版日期:2011-03-17 发布日期:2011-03-16
通讯作者: 王军。E-mail: junw@xmu.edu.cn
作者简介:郭明兰(1980—), 女, 江西省吉安市人, 博士, 主要从事海洋动物生化遗传研究。E-mail: guominglan03@yahoo.com.cn
基金资助:
国家自然科学基金项目(30070595); 国家“863”计划项目(2006AA10A414)

Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae

GUO Ming-lan1, YOU Xin-xin2, SU Yong-quan2, DING Shao-xiong2, WANG Jun2

1. Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, CAS, Guangzhou 510301, China; 2. Department of Oceanography and Institute of Subtropical Oceanography, Xiamen University, Xiamen 361005, China

Received:2009-05-12 Revised:2010-06-22 Online:2011-03-17 Published:2011-03-16
Contact: 王军。E-mail: junw@xmu.edu.cn
About author:郭明兰(1980—), 女, 江西省吉安市人, 博士, 主要从事海洋动物生化遗传研究。E-mail: guominglan03@yahoo.com.cn
Supported by:
国家自然科学基金项目(30070595); 国家“863”计划项目(2006AA10A414)

摘要/Abstract

摘要：

实验采用植物血凝素、秋水仙碱腹腔注射, 肾细胞直接制片法分析了川纹笛鲷Lutjanus sebae染色体核型、Ag-NORs和C-带。结果表明：1)川纹笛鲷二倍体染色体数2n=48, 核型公式为: 2n=48t, NF=48; 在第1对染色体靠近着丝粒部位有明显的次缢痕。2)染色体经快速银染后, Ag-NORs的数目在不同细胞中表现出多态性, 数目1— 4个, 2个Ag-NORs的频率最高(占79%)。在分裂相中, 第1对t染色体近着丝粒的次缢痕区均出现2个银染位点(Ag-NORs阳性), 且未见Ag-NORs的联合现象。3)大多数染色体的着丝粒区显示出1个深浅不同的C-带, 在第1对染色体的随体区域分布有大量的结构异染色质, 表现C-带强阳性。讨论了鱼类核型演化规律和Ag-NORs、C-带的发生机制, 以及川纹笛鲷的进化地位。

关键词: 川纹笛鲷Lutjanus sebae, 核型, Ag-NORs, C-带

Abstract:

Studies on karyotype, Ag-NORs, and C-banding of Lutjanus sebae were performed. The chromosomes were re-ceived from the head kidney by using a method of injecting with PHA and colchicines, air drying, and Giemsa staining. The results were as follows. 1) The karyotypic formula of L.sebae was 2n=48t, NF=48. Meanwhile, a pair of chromosomes, with secondary constriction near the centromere, was found on chromosome 1. 2) The Ag-NORs polymorphisms were individually specific in this fish. The silver staining pots were 1-4, and the number of Ag-NORs was mainly 2 (79%). A pair of nucleolar organizer regions was observed on the secondary constriction of chromosome 1, and no Ag-NORs combination was found. 3) Centromeres of most chromosomes were C-bandings positive, and heterochromatin was detected at the satellite zone of chro-mosome 1. Thus, we discussed the evolution of karyotype, the developing mechanism of Ag-NORs, and C-bandings for fish. The phylogenetic condition of L. sebae was evaluated as well.

Key words: Lutjanus seba, karyotype, Ag-NORs, C-banding

郭明兰,游欣欣,苏永全,丁少雄,王军. 川纹笛鲷染色体核型、银染和C-带[J]. 热带海洋学报, 2011, 30(1): 91-95.

GUO Ming-lan,YOU Xin-xin,SU Yong-quan,DING Shao-xiong,WANG Jun. Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae[J]. Journal of Tropical Oceanography, 2011, 30(1): 91-95.

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川纹笛鲷染色体核型、银染和C-带

Studies on chromosome karyotype, Ag-NORs and C-banding patterns of Lutjanus sebae

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