红海榄幼苗根系呼吸代谢对水淹胁迫的响应

doi:10.11978/2021189

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (6): 12-19.doi: 10.11978/2021189CSTR: 32234.14.2021189

红海榄幼苗根系呼吸代谢对水淹胁迫的响应

程皓¹^,²^,³(), 王友绍¹^,²^,³(), 马晓宇¹^,²^,³

1.热带海洋环境国家重点实验室(中国科学院南海海洋研究所), 广东广州 510301
2.中国科学院大亚湾海洋生物综合试验站, 广东深圳 518121
3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458

收稿日期:2021-12-31 修回日期:2022-04-11 出版日期:2022-11-10 发布日期:2022-04-14
通讯作者: 王友绍
作者简介:程皓(1984—), 男, 安徽省岳西县人, 研究员, 从事红树林生态学研究。email: chenghao@scsio.ac.cn
基金资助:
国家重点研发计划项目(国家科技基础资源调查专项)(2017FY100700);国家自然科学基金重点项目(41676086);国家自然科学基金重点项目(U1901211);国家自然科学基金重点项目(41876126);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0305);南方海洋科学与工程广东省实验室(广州)人才团队引进重大专项(GML2019ZD0303);国际伙伴计划(133244KYSB20180012);中国科学院A类战略性先导科技专项(XDA23050200);中国科学院A类战略性先导科技专项(XDA19060201)

Response of respiratory metabolism in the roots of Rhizophora stylosa to waterlogging

CHENG Hao¹^,²^,³(), WANG Youshao¹^,²^,³(), MA Xiaoyu¹^,²^,³

1. State Key Laboratory of Tropical Oceanography (South China Sea Institute of Oceanology, Chinese Academy of Sciences), Guangzhou 510301, China
2. Daya Bay Marine Biology Research Station, Chinese Academy of Sciences, Shenzhen 518121, China
3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

Received:2021-12-31 Revised:2022-04-11 Online:2022-11-10 Published:2022-04-14
Contact: WANG Youshao
Supported by:
National Key Research and Development Program of China(2017FY100700);National Natural Science Foundation of China(41676086);National Natural Science Foundation of China(U1901211);National Natural Science Foundation of China(41876126);Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0305);Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0303);International Partnership Program of Chinese Academy of Sciences(133244KYSB20180012);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA23050200);Strategic Priority Research Program of the Chinese Academy of Sciences(XDA19060201)

摘要/Abstract

摘要：

本研究利用人工潮汐系统(每天淹水0、6、12和18h), 研究了红海榄幼苗根系呼吸代谢对水淹胁迫的响应。结果表明, 每天淹水6h对红海榄幼苗的生长影响不大, 但随着水淹时间的延长, 红海榄幼苗的生长显著受到抑制。水淹时间的延长同样明显抑制了红海榄幼苗根系的产能效率, 且根系三羧酸循环的有氧呼吸速率、三磷酸腺苷含量以及琥珀酸脱氢酶和苹果酸脱氢酶活性最小值均出现在每天水淹18h处理组中。相反, 水淹胁迫下红海榄幼苗根系乙醇脱氢酶、乳酸脱氢酶活性以及乙醇和乳酸含量都呈上升的趋势。综上, 尽管无氧呼吸可一定程度上缓解三羧酸循环受阻所导致的产能匮乏, 但是过度的水淹胁迫仍会扰乱红海榄幼苗正常的生长和代谢, 导致三磷酸腺苷产能效率的降低以及无氧发酵代谢产物的积累。

关键词: 水淹胁迫, 红海榄, 无氧呼吸, 三羧酸循环

Abstract:

In this study, artificial-tidal systems (0, 6, 12, 18 h·d^-1) were employed to explore the effect of waterlogging on respiratory metabolism in the roots of Rhizophora stylosa. The results showed that 6 h·d^-1waterlogging treatment appeared to has little negative effect on plant growth. However, the biomass yield efficiency decreased significantly with the elongation of waterlogging duration. Tricarboxylic acid respiratory rate, adenosine-triphosphate content, and the activities of succinate dehydrogenase and malate dehydrogenase were also obviously inhibited by long-duration waterlogging, especially in the treatment of 18 h·d^-1. On the contrary, alcohol dehydrogenase, lactate dehydrogenase, and the contents of alcohol and lactate increased with the elongation of waterlogging duration. In summary, although anaerobic respiration can partly alleviate energy deficiency caused by the inhibition of tricarboxylic acid cycle, excessive waterlogging would disrupt normal growth and metabolism of R. stylosa, leading to declined adenosine-triphosphate productivity and increased anaerobic fermentative metabolites.

Key words: waterlogging, Rhizophora stylosa, anaerobic respiration, tricarboxylic acid cycle

中图分类号:

Q945.78

程皓, 王友绍, 马晓宇. 红海榄幼苗根系呼吸代谢对水淹胁迫的响应[J]. 热带海洋学报, 2022, 41(6): 12-19.

CHENG Hao, WANG Youshao, MA Xiaoyu. Response of respiratory metabolism in the roots of Rhizophora stylosa to waterlogging[J]. Journal of Tropical Oceanography, 2022, 41(6): 12-19.

图/表 5

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水淹处理/(h·d^-1)	地下根系生物量/g	地上茎叶生物量/g	总生物量/g	地下根系生物量/地上茎叶生物量
0	3.40 ± 0.18^a	4.19 ± 0.14^a	7.59 ± 0.31^a	0.81 ± 0.02^a
6	3.41 ± 0.09^a	4.45 ± 0.19^a	7.86 ± 0.26^a	0.77 ± 0.03^b
12	2.79 ± 0.14^b	4.22 ± 0.13^a	7.01 ± 0.27^b	0.66 ± 0.02^c
18	2.26 ± 0.10^c	3.79 ± 0.10^b	6.05 ± 0.20^c	0.59 ± 0.01^d

红海榄幼苗根系呼吸代谢对水淹胁迫的响应

Response of respiratory metabolism in the roots of Rhizophora stylosa to waterlogging

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