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热带海洋学报 ›› 2010, Vol. 29 ›› Issue (4): 71-75.doi: 10.11978/j.issn.1009-5470.2010.04.071cstr: 32234.14.j.issn.1009-5470.2010.04.071

• 海洋生物学 • 上一篇    下一篇

中国南海Bukholderia sp. DA2菌株降解邻苯二甲酸二甲酯研究

王亚丽1,2, 殷波1, 洪义国1, 顾继东1   

  1. 1. 中国科学院南海海洋研究所热带海洋环境动力学重点实验室, 广东 广州 510301; 2. 广东省博物馆, 广东 广州 510110
  • 收稿日期:2008-04-10 修回日期:2008-06-06 出版日期:2010-07-31 发布日期:2010-07-29
  • 作者简介:王亚丽(1978—), 女, 山东省莱州市人, 博士研究生, 专业方向为环境与分子微生物学。
  • 基金资助:

    广东省自然科学基金博士启动项目(06301430); 国家自然科学基金(30800032)

Biodegradation of dimethyl phthalate by Burkholderia sp. DA2 isolated from marine sediments in the South China Sea

WANG Ya-li1,2, YIN Bo1, HONG Yi-guo1, GU Ji-dong1   

  1. 1. Key laboratory of Tropical Marine Environment Dynamic, South China Sea Institute of Oceanography, CAS, Guangzhou 510301, China; 2. Guangdong Museum, Guangzhou 510110, China
  • Received:2008-04-10 Revised:2008-06-06 Online:2010-07-31 Published:2010-07-29
  • About author:王亚丽(1978—), 女, 山东省莱州市人, 博士研究生, 专业方向为环境与分子微生物学。
  • Supported by:

    广东省自然科学基金博士启动项目(06301430); 国家自然科学基金(30800032)

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2

摘要：

以邻苯二甲酸二甲酯(dimethyl phthalate, DMP)为惟一碳源, 从中国南海底泥中分离筛选到一株高效降解菌株, 即伯克霍尔德氏菌Bukholderia. sp. DA2。该菌株降解DMP及其邻苯二甲酸二甲酯(Dimethyl phthalate, DMT)和间苯二甲酸二甲酯(Dimethyl dimethyl isophthalate, DMI) 2种异构体的效果显著。B. sp. DA2菌株能够在8d内好氧降解矿化100mg·L-1的DMT, 15d内好氧降解矿化400mg·L-1的DMP。但对于200mg·L-1 的DMI, 则不能彻底降解矿化, 反应终止在生成间苯二甲酸一甲酯中间产物的阶段。研究表明, B. sp.DA2菌株降解DMT及DMP的途径相似, 首先脱酯生成单酯和甲醇, 继而水解酸化, 直至彻底矿化。降解速率及其途径, 与化学结构密切相关。

关键词: 海洋微生物, 生物降解, 邻苯二甲酸二甲酯, 间苯二甲酸二甲酯, 对苯二甲酸二甲酯

Abstract:

 Burkholderia sp. DA2 strain isolated from marine sediments in the South China Sea was capable of utilizing dimethyl phthalate (DMP) as the sole source of carbon and energy for biodegradation of DMP, DMT (dimethyl phthalate) and DMI (dimethyl isophthalate). During the transformation of DMP (400mg·L-1), its corresponding intermediates were identified as mono-methyl phthalate (MMP) and phthalate acid (PA) by HPLC analysis. By the 9th day, the DMP concentration was undetectable in the culture, corresponding to a DMP decrease; MMP accumulated to 38.9 mg/L during eight days, at the same time amount of PA was also produced during the first 12 days of degradation; this PA was completely degraded after 15 days. During the transformation of DMT (100mg·L-1), 80% DMT was transferred into MMT in one day, but the MMT could not degraded rapidly and accumulated to 80.2mg·L-1, and completely mineralized during eight days. During the DMI (200mg·L-1) degradation, the DMI was transferred into MMI after six days, but the MMI could not further mineralize. Maybe the compounds chemical structures infect the activity of the enzyme degradation. The biodegradation pathway of DMP, DMT and DMI was proposed as DMP\DMT\DMI→MMP\MMT\MMI→PA\TA\IA→ ... →CO2+H2O.

Key words: DMP, DMI, DMT, biodegradation, marine microorganism