一株海绵共生真菌ZSDS1-F11次生代谢产物的研究

doi:10.11978/2015011

热带海洋学报 ›› 2015, Vol. 34 ›› Issue (5): 36-39.doi: 10.11978/2015011CSTR: 32234.14.2015011

一株海绵共生真菌ZSDS1-F11次生代谢产物的研究

贺卫军^{1, 2}, 王俊锋², 林秀萍², 周小江¹, 刘永宏²

1. 湖南中医药大学药学院, 湖南长沙 410208;
2.中国科学院南海海洋研究所热带海洋生物资源与生态重点实验室, 广东广州 510301

收稿日期:2015-01-20 修回日期:2015-02-11 出版日期:2015-09-11 发布日期:2015-09-11
基金资助:
国家自然科学基金项目(31270402、31170336); 中国极地研究中心(上海) 国家海洋局极地科学重点实验室开放课题项目(KP201305)

Study on the secondary metabolites from a sponge-derived fungus ZSDS1-F11

HE Wei-jun^{1, 2}, WANG Jun-feng², LIN Xiu-ping², ZHOU Xiao-jiang¹, LIU Yong-hong²

1. School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China;
2.Key Laboratory of tropical marine bio-resources and ecology, South China Sea Institute of Oceanology, Chinese Academy of

Received:2015-01-20 Revised:2015-02-11 Online:2015-09-11 Published:2015-09-11

摘要/Abstract

摘要： 对分离自西沙棕色扁海绵PhakelliafuscaThiele的共附生真菌ZSDS1-F11的次生代谢产物进行了研究; 运用硅胶柱层析、Sephadex LH-20凝胶柱层析以及半制备高效液相等方法对菌株ZSDS1-F11的发酵产物进行分离纯化; 通过理化性质及波谱学方法及参阅文献进行化学结构鉴定; 采用环氧合酶-1和环氧合酶-2(cyclooxygenase, COX-1, COX-2)为分子靶标进行炎症抑制剂筛选。结果从菌株ZSDS1-F11的发酵产物中分离得到8个单体化合物。其中化合物1和2具有较弱的环氧合酶-1(cyclooxygenase, COX-1)抑制活性, IC₅₀分别为27.2和2.93μmol。菌株ZSDS1-F11能够产生结构多样性的活性次生代谢产物, 并首次报道了化合物1和2具有较弱的抗炎活性。

关键词: 棕色扁海绵, 海洋真菌, 次生代谢产物, 抗炎活性

Abstract: The secondary metabolites of strain ZSDS1-F11, an endophytic fungus isolated from the sponge Phakelliafusca Thiele, were investigated. Fermented products of ZSDS1-F11 were purified by means of chromatography over silica gel column, Sephadex LH-20 and semi-preparative HPLC. Compounds were confirmed based on their physical and chemical properties, spectroscopic analysis and comparison with the relevant literature. Using COX-1 and COX-2 as molecular targets for inflammation inhibitor screening, eight compounds were obtained from the fermented products of strain ZSDS1-F11. Compounds 1 and 2 showed weak inhibitory activity against enzyme COX-1, their IC₅₀values were 27.2 and 2.93 μmol, respectively. The strain ZSDS1-F11 produced diverse bioactive secondary metabolites. This is the first report on compounds 1 and 2 with weak anti-inflammatory activity.

Key words: Sciences, Guangzhou 510301, China

贺卫军, 王俊锋, 林秀萍, 周小江, 刘永宏. 一株海绵共生真菌ZSDS1-F11次生代谢产物的研究[J]. 热带海洋学报, 2015, 34(5): 36-39.

HE Wei-jun, WANG Jun-feng, LIN Xiu-ping, ZHOU Xiao-jiang, LIU Yong-hong. Study on the secondary metabolites from a sponge-derived fungus ZSDS1-F11[J]. Journal of Tropical Oceanography, 2015, 34(5): 36-39.

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一株海绵共生真菌ZSDS1-F11次生代谢产物的研究

Study on the secondary metabolites from a sponge-derived fungus ZSDS1-F11

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