涠洲岛海洋沉积物来源真菌Aspergillus sp. GXIMD02003的代谢产物研究

doi:10.11978/2022232

热带海洋学报 ›› 2023, Vol. 42 ›› Issue (5): 154-160.doi: 10.11978/2022232CSTR: 32234.14.2022232

涠洲岛海洋沉积物来源真菌Aspergillus sp. GXIMD02003的代谢产物研究

邢楠楠¹^,²(), 任润馨¹^,³, 唐振洲¹^,³, 罗志宏¹^,³, 夏辰曦¹^,³, 刘永宏¹^,³, 彭亮⁴(), 陈显强¹^,³()

1. 广西中医药大学海洋药物研究院, 广西南宁 530200
2. 广西中医药大学广西中医基础研究重点实验室, 广西南宁 530200
3. 广西中医药大学广西海洋药物重点实验室, 广西南宁 530200
4. 江西科技师范大学药学院江西省高等学校精细化学品工程技术研究中心, 江西南昌 330013

收稿日期:2022-11-01 修回日期:2022-12-30 出版日期:2023-09-10 发布日期:2023-01-03
作者简介:
邢楠楠(1984—), 女, 助理研究员, 主要从事天然药物活性评价研究。email: 37597931@qq.com
基金资助:
广西科技基地和人才专项(桂科AD21075016); 广西自然科学基金项目(2020GXNSFAA297163); 广西自然科学基金项目(2020GXNSFGA297002); 广西八桂学者专项(05019055); 江西省高等学校精细化学品工程技术研究中心开放基金项目(JFCEC-KF-2101); 广西中医药大学海洋药物研究院团队科研专项(2018ZD005-A02); 广西中医基础研究重点实验室自主研究课题项目(22-065-53-01)

Study on the secondary metabolites of fungus Aspergillus sp. GXIMD02003 derived from marine sediment in the Weizhou island

XING Nannan¹^,²(), REN Runxin¹^,³, TANG Zhenzhou¹^,³, LUO Zhihong¹^,³, XIA Chenxi¹^,³, LIU Yonghong¹^,³, PENG Liang⁴(), CHEN Xianqiang¹^,³()

1. Institute of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
2. Guangxi Key Laboratory of Chinese Medicine Foundation Research, Guangxi University of Chinese Medicine, Nanning 530200, China
3. Guangxi Key Laboratory of Marine Drugs, Guangxi University of Chinese Medicine, Nanning 530200, China
4. Engineering Center for Fine Chemicals, School of Pharmacy, Jiangxi Science & Technology Normal University, Nanchang 330013, China

Received:2022-11-01 Revised:2022-12-30 Online:2023-09-10 Published:2023-01-03
Supported by:
Guangxi Science and Technology Base and Talent Special Project(AD21075016); Natural Science Foundation of Guangxi Province(2020GXNSFAA297163); Natural Science Foundation of Guangxi Province(2020GXNSFGA297002); Special Fund for Bagui Scholars of Guangxi(05019055); Open Project of Engineering Center of Jiangxi University for Fine Chemicals(JFCEC-KF-2101); Special Scientific Research Fund for Team of Institute of Marine Drugs of Guangxi University of Chinese Medicine(2018ZD005-A02); Project of Guangxi Key Laboratory of Chinese Medicine Foundation Research(22-065-53-01)

摘要/Abstract

摘要：

为了探讨海洋沉积物来源真菌Aspergillus sp. GXIMD02003次级代谢产物的多样性, 运用多种色谱技术分离该菌株代谢产物, 基于质谱、核磁共振波谱和旋光等数据结合文献数据比对鉴定化合物结构。8个化合物从真菌Aspergillus sp. GXIMD02003中分离并被鉴定为(3S,11aS)-3-[(1H-indol-3-yl)methyl]-7,9-dihydroxy-8-methoxy-2,3,11,11a-tetrahydro-6H-pyrazino[1,2-b]isoquinoline-1,4-dione (1)、cyclo(L-Pro-L-Tyr) (2)、cyclo(L-4-Hyp-D-phe) (3)、cyclo(L-4-Hyp-L-Phe) (4)、cyclo(L-4-Hyp-L-leucine) (5)、cyclo(L-4-Hyp-D-leucine) (6)、desmethyldiaportinol (7)、2-(2ʹ-hydroxypropyl)-5-methyl-7-hydroxychromone (8)。化合物1~8在测试浓度下未显示抑制癌细胞增殖、α-葡萄糖酶和抑菌活性。化合物1~8首次从涠洲岛海洋沉积物来源真菌中分离得到。

关键词: 海洋真菌, Aspergillus, 次级代谢产物, 洋沉积物, 涠洲岛

Abstract:

To explore the diversity of secondary metabolites of fungus Aspergillus sp. GXIMD02003 derived from marine sediment, its secondary metabolites were separated based on the various chromatographic techniques. Chemical structures were identified using mass spectroscopy, nuclear magnetic resonance spectroscopy, and optical rotation combined with comparison of literature data. Eight compounds were isolated from Aspergillus sp. GXIMD02003, and identified as (3S, 11aS)-3-[(1H-indol-3-yl)methyl]-7, 9-dihydroxy-8-methoxy-2,3,11,11a-tetrahydro-6H-pyrazino[1,2-b]isoquinoline-1,4-dione (1), cyclo(L-Pro-L-Tyr) (2), cyclo(L-4-Hyp-D-phe) (3), cyclo(L-4-Hyp-L-Phe) (4), cyclo(L-4-Hyp-L-leucine) (5), cyclo(L-4-Hyp-D-leucine) (6), desmethyldiaportinol (7), 2-(2ʹ-hydroxypropyl)-5-methyl-7-hydroxychromone (8). Compounds 1~8 showed no inhibitory activities against cancer cell proliferation, α-glucosidase and pathogenic bacteria at testing concentration. Compounds 1~8 were isolated from fungus derived from marine sediment of the Weizhou island for the first time. The project enriched the diversity of secondary metabolites from Aspergillus sp. GXIMD02003, laid a foundation for exploring the bioactivity of fungal secondary metabolites from marine sediment in the Weizhou island.

Key words: marine fungus, Aspergillus, secondary metabolites, marine sediment, Weizhou Island

邢楠楠, 任润馨, 唐振洲, 罗志宏, 夏辰曦, 刘永宏, 彭亮, 陈显强. 涠洲岛海洋沉积物来源真菌Aspergillus sp. GXIMD02003的代谢产物研究[J]. 热带海洋学报, 2023, 42(5): 154-160.

XING Nannan, REN Runxin, TANG Zhenzhou, LUO Zhihong, XIA Chenxi, LIU Yonghong, PENG Liang, CHEN Xianqiang. Study on the secondary metabolites of fungus Aspergillus sp. GXIMD02003 derived from marine sediment in the Weizhou island[J]. Journal of Tropical Oceanography, 2023, 42(5): 154-160.

图/表 1

参考文献 27

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涠洲岛海洋沉积物来源真菌Aspergillus sp. GXIMD02003的代谢产物研究

Study on the secondary metabolites of fungus Aspergillus sp. GXIMD02003 derived from marine sediment in the Weizhou island

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