球形棕囊藻(Phaeocystis globosa Scherffel)细胞形态转变及转录组比较研究
王西艳(1998—), 女, 硕士研究生, 主要从事海洋微藻研究。email: |
Copy editor: 殷波
收稿日期: 2023-11-24
修回日期: 2023-12-27
网络出版日期: 2024-01-08
基金资助
国家自然科学基金项目(41976114)
国家自然科学基金项目(42376159)
国家环境保护近岸海域生态环境重点实验室开放课题项目(202311)
Comparative study on the cell morphological transformation and transcriptome of Phaeocystis globosa Scherffel
Received date: 2023-11-24
Revised date: 2023-12-27
Online published: 2024-01-08
Supported by
National Natural Science Foundation of China(41976114)
National Natural Science Foundation of China(42376159)
Open Project of the National Key Laboratory for Environmental Protection of Coastal Ecological Environment(202311)
球形棕囊藻是沿海重要且常见的有害赤潮原因种。文章对分离自广西北部湾的一株球形棕囊藻进行了不同细胞形态学和转录组学分析。结果表明, 球形棕囊藻具有2种类型的细胞, 一种是具有鞭毛的游动细胞, 另一种是不具鞭毛的不动细胞, 大小介于2~5μm, 不动细胞能够形成囊体, 直径从几微米到几百微米不等。通过转录组分析发现, 囊体细胞与单细胞相比有更多的基因发生上调表达, 且大部分差异表达基因与运输、分解代谢、氨基酸、脂质、碳水化合物等代谢途径相关, 尤其是与聚糖生物合成有关的UDP-葡萄糖醛酸-4-表异构酶(uridine diphosphate-glucose-4-epimerase)编码基因显著上调表达, 反映囊体基质形成过程中糖胺聚糖的大量合成。
王西艳 , 薛玥 , 孟不凡 , 付峰 , 沈萍萍 . 球形棕囊藻(Phaeocystis globosa Scherffel)细胞形态转变及转录组比较研究[J]. 热带海洋学报, 2024 , 43(5) : 49 -57 . DOI: 10.11978/2023174
Phaeocystis globosa is an important and cosmopolitan harmful alga in coastal area. This study conducted a comparative analysis on the cell morphological characteristics and transcriptome of P. globosa isolated from the Beibu Gulf of Guangxi Province. The results indicate that P. globosa contains two types of cells: motile flagellated cells and non-motile cells without flagella. The cell size is 2-5 μm and the non-motile cells are capable of forming colonies, with diameters ranging from few micrometers to several hundred micrometers. Transcriptome analysis reveals that colonial cells exhibit more genes with upregulated expression than solitary cells and most of the differentially expressed genes are associated with transport, decomposition metabolism, amino acids, lipids, carbohydrates, and other metabolic pathways. Particularly, the gene encoding UDP-glucose-4-epimerase, which is involved in polysaccharide biosynthesis, shows significantly upregulated expression, indicating extensive synthesis of glycosaminoglycans during the formation of colony. These results provide basic information for further understanding the morphological transformation mechanism of P. globosa in different geographical strains.
Key words: Phaeocystis globosa; solitary cells; colony; transcriptome
图1 球形棕囊藻的生长状况a. 单细胞生长曲线; b. 囊体生长曲线; c. 囊体直径与生长天数的关系; d. 囊体细胞数量与其直径的相关性 Fig. 1 Growth status of Phaeocystis globosa. (a) Growth curve of solitary cells; (b) growth curve of colonies; (c) relationship between colonial diameter and culture time; (d) correlation between the number of colonial cells and diameter |
图3 北部湾株球形棕囊藻囊体细胞形态a. 较大的囊体; b. 新生成的小囊体; c. 极性囊体; d. 内部细胞逸出的囊体; e. 细胞聚集体, 无囊体边界; f. 聚集体中具鞭毛细胞 Fig. 3 Colonial cells morphology of Phaeocystis globosa Beibu Gulf strain. (a) Larger colony; (b) newly formed small colony; (c) polarized colony; (d) colony with internal cells escape; (e) cell aggregate without colonial boundaries; (f) cells with flagella in the aggregate |
图4 北部湾株球形棕囊藻单细胞电镜扫描形态a和b. 具有2根长鞭毛及1根定鞭毛的单细胞; c. 具有凹陷的无鞭毛单细胞; d. 具有2条短鞭毛突起的单细胞 Fig. 4 Solitary cell morphology of Phaeocystis globosa Beibu Gulf strain under scanning electron microscopy. (a) and (b) Solitary cell with flagella and haptonema; (c) solitary cell with concave shape and no flagella; (d) solitary cell with two short flagella protrusions |
图5 球形棕囊藻不同形态细胞差异基因表达量的变化a. 囊体和单细胞的差异表达基因数量统计图, 筛选条件为Padjust≤0.05并且|log2FC|≥1; b. 囊体和单细胞的差异表达基因的火山图, 显示不同样本间基因表达差异程度。log2FC中的FC即差异倍数(fold change), 表示两样品(组)间表达量的比值, 对其取以2为底的对数之后即为log2FC, 另外用P值的负对数(-Log10(P-value))来表示P值的显著性; 红色表示上调基因, 蓝色表示下调基因 Fig. 5 Changes in differential gene expression levels in different cells of Phaeocystis globosa. (a) Statistical diagram of the number of differentially expressed genes between colonial cells and solitary cells, with screening conditions of Padjust ≤ 0.05 and | log2FC | ≥ 1; (b) volcano map of differentially expressed genes between colonial and solitary cells, showing the degree of gene expression differences between different samples. The FC in log2FC is fold change, which represents the ratio of expression between two samples (groups), and is log2FC after taking the logarithm with 2 as the base, additionally the negative logarithm of the P-value (-Log10(P-value)) was used to indicate the significance of the P-value; Red indicates upregulation of genes, while blue indicates downregulation of genes |
图6 球形棕囊藻囊体细胞和单细胞的差异基因KEGG功能注释和富集通路分析a. 囊体和单细胞的差异基因功能注释图; b. 囊体和单细胞的差异表达基因KEGG富集通路图(前20) Fig. 6 Functional annotation and enrichment pathway analysis of the differential gene KEGG between the colonial and solitary cells of Phaeocystis globosa. (a) Function annotation diagram of differentially expressed genes between the colonial and solitary cells; (b) KEGG enrichment pathway diagram of differentially expressed genes in the colonial and solitary cells (top 20) |
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