球形棕囊藻(Phaeocystis globosa Scherffel)细胞形态转变及转录组比较研究

doi:10.11978/2023174

摘要/Abstract

摘要：

球形棕囊藻是沿海重要且常见的有害赤潮原因种。文章对分离自广西北部湾的一株球形棕囊藻进行了不同细胞形态学和转录组学分析。结果表明, 球形棕囊藻具有2种类型的细胞, 一种是具有鞭毛的游动细胞, 另一种是不具鞭毛的不动细胞, 大小介于2~5μm, 不动细胞能够形成囊体, 直径从几微米到几百微米不等。通过转录组分析发现, 囊体细胞与单细胞相比有更多的基因发生上调表达, 且大部分差异表达基因与运输、分解代谢、氨基酸、脂质、碳水化合物等代谢途径相关, 尤其是与聚糖生物合成有关的UDP-葡萄糖醛酸-4-表异构酶(uridine diphosphate-glucose-4-epimerase)编码基因显著上调表达, 反映囊体基质形成过程中糖胺聚糖的大量合成。

关键词: 球形棕囊藻, 单细胞, 囊体, 转录组

Abstract:

Phaeocystis globosa is an important and cosmopolitan harmful alga in coastal area. This study conducted a comparative analysis on the cell morphological characteristics and transcriptome of P. globosa isolated from the Beibu Gulf of Guangxi Province. The results indicate that P. globosa contains two types of cells: motile flagellated cells and non-motile cells without flagella. The cell size is 2-5 μm and the non-motile cells are capable of forming colonies, with diameters ranging from few micrometers to several hundred micrometers. Transcriptome analysis reveals that colonial cells exhibit more genes with upregulated expression than solitary cells and most of the differentially expressed genes are associated with transport, decomposition metabolism, amino acids, lipids, carbohydrates, and other metabolic pathways. Particularly, the gene encoding UDP-glucose-4-epimerase, which is involved in polysaccharide biosynthesis, shows significantly upregulated expression, indicating extensive synthesis of glycosaminoglycans during the formation of colony. These results provide basic information for further understanding the morphological transformation mechanism of P. globosa in different geographical strains.

Key words: Phaeocystis globosa, solitary cells, colony, transcriptome

王西艳, 薛玥, 孟不凡, 付峰, 沈萍萍. 球形棕囊藻(Phaeocystis globosa Scherffel)细胞形态转变及转录组比较研究[J]. 热带海洋学报, 2024, 43(5): 49-57.

WANG Xiyan, XUE Yue, MENG Bufan, FU Feng, SHEN Pingping. Comparative study on the cell morphological transformation and transcriptome of Phaeocystis globosa Scherffel[J]. Journal of Tropical Oceanography, 2024, 43(5): 49-57.

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