本文对石花菜共附生真菌Aspergillus fumigatus 9-1次级代谢产物进行结构和活性的研究。通过薄层色谱、硅胶柱色谱、高效液相色谱、凝胶色谱等对石花菜共附生真菌Aspergillus fumigatus9-1 次级代谢产物进行分离纯化，从中得到了16个化合物。通过核磁共振波谱等波谱分析方法及结合文献对照，鉴定了化合物结构：12R,13S-dihydroxyfumitremorgin C (1)、helvolic acid (2)、questin (3)、pyripyropene A (4)、verruculogen TR-2 (5)、cyclotryprostatin E (6)、cyclotryprostatin B (7)、asperfumigatin (8)、spirocyclic diketopiperazine alkaloid (9)、fumigaclavine C (10)、monomethylsulochrin (11)、thymidine (12)、fumigaclavine A (13)、chaetominine (14)、prenylcyclotryprostatin A (15)、fumiquinazoline J (16)。化合物15为首次从Aspergillus中发现。化合物2对L. monocytogenes具有较强的抗菌活性，MIC为4.03μg·mL−1。化合物3、4、11对单增李斯特菌具有中等抗菌活性，MIC分别为64.54μg·mL−1、66.25μg·mL−1、78.63μg·mL−1。体外活性抗氧化活性结果表明，化合物1具有较强的DPPH自由基清除活性，化合物1、10、11、13具有较强的ABTS自由基清除活性。
何鸣凤
,
黄佳翔
,
田永奇
.
石花菜共附生烟曲霉Aspergillus
fumigatus 9-1次级代谢产物抗菌、抗氧化研究[J]. 热带海洋学报, 0
: 1
.
DOI: 10.11978/2024182
The secondary metabolites produced by Aspergillus fumigatus 9-1, isolated from the red alga Gelidium, were systematically separated and purified using a combination of TLC, silica gel column chromatography, HPLC, and gel column chromatography, resulting in the isolation of 16 compounds. Spectral analysis, including NMR spectroscopy and comparison with literature data, led to the structural elucidation of these compounds as: 12R,13S-dihydroxyfumitremorgin C (1), helvolic acid (2), questin (3), pyripyropene A (4), verruculogen TR-2 (5), cyclotryprostatin E (6), cyclotryprostatin B (7), asperfumigatin (8), spirocyclic diketopiperazine alkaloid (9), fumigaclavine C (10), monomethylsulochrin (11), thymidine (12), fumigaclavine A (13), chaetominine (14), prenylcyclotryprostatin A (15), fumiquinazoline J (16). Compound 15 was first discovered from the genus Aspergillus. Compound 2 exhibited potent antibacterial activity against Listeria monocytogenes, with a minimum inhibitory concentration (MIC) of 4.03 μg·mL -1. Compound 3, 4 and11 demonstrated moderate antibacterial activity against L. monocytogenes, with MIC values of 64.54 μg·mL -1, 66.25 μg·mL -1, and 78.63 μg·mL -1, respectively. Compound 1 displayed strong DPPH radical scavenging activity. Furthermore, compounds 1, 10, 11, and 13 exhibited potent ABTS radical scavenging activity.
