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热带海洋学报

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石花菜共附生烟曲霉Aspergillus fumigatus 9-1次级代谢产物抗菌、抗氧化研究

何鸣凤, 黄佳翔, 田永奇   

  1. 福州大学生物科学与工程学院 福州 350108

  • 收稿日期:2024-09-24 修回日期:2024-11-01 接受日期:2024-11-25
  • 通讯作者: 田永奇
  • 基金资助:
    国家自然科学基金(42006094); 福建省自然科学基金(2022J01561); 福建省-印尼海洋食品联合研发中心开放课题(Y1-KF2201)

Research on the antioxidant activity and antibacterial activity of metabolites from Gelidium-derived fungus Aspergillus fumigatus 9-1

HE Mingfeng, HUANG Jiaxiang, TIAN Yongqi   

  1. College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, China

  • Received:2024-09-24 Revised:2024-11-01 Accepted:2024-11-25
  • Supported by:

     National Natural Science Foundation of China (42006094); Fujian Natural Science Foundation (2022J01561); Fujian Province-Indonesia Marine Food Joint Research and Development Center Open-Ended Foundation (Y1-KF2201).

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摘要： 本文对石花菜共附生真菌Aspergillus fumigatus 9-1次级代谢产物进行结构和活性的研究。通过薄层色谱、硅胶柱色谱、高效液相色谱、凝胶色谱等对石花菜共附生真菌Aspergillus fumigatus9-1 次级代谢产物进行分离纯化，从中得到了16个化合物。通过核磁共振波谱等波谱分析方法及结合文献对照，鉴定了化合物结构：12R,13S-dihydroxyfumitremorgin C (1)、helvolic acid (2)、questin (3)、pyripyropene A (4)、verruculogen TR-2 (5)、cyclotryprostatin E (6)、cyclotryprostatin B (7)、asperfumigatin (8)、spirocyclic diketopiperazine alkaloid (9)、fumigaclavine C (10)、monomethylsulochrin (11)、thymidine (12)、fumigaclavine A (13)、chaetominine (14)、prenylcyclotryprostatin A (15)、fumiquinazoline J (16)。化合物15为首次从Aspergillus中发现。化合物2对L. monocytogenes具有较强的抗菌活性，MIC为4.03μg·mL−1。化合物3、4、11对单增李斯特菌具有中等抗菌活性，MIC分别为64.54μg·mL−1、66.25μg·mL−1、78.63μg·mL−1。体外活性抗氧化活性结果表明，化合物1具有较强的DPPH自由基清除活性，化合物1、10、11、13具有较强的ABTS自由基清除活性。

关键词: 石花菜共附生真菌, 次级代谢产物, 抗菌活性, 抗氧化活性

Abstract: The secondary metabolites produced by Aspergillus fumigatus 9-1, isolated from the red alga Gelidium, were systematically separated and purified using a combination of TLC, silica gel column chromatography, HPLC, and gel column chromatography, resulting in the isolation of 16 compounds. Spectral analysis, including NMR spectroscopy and comparison with literature data, led to the structural elucidation of these compounds as: 12R,13S-dihydroxyfumitremorgin C (1), helvolic acid (2), questin (3), pyripyropene A (4), verruculogen TR-2 (5), cyclotryprostatin E (6), cyclotryprostatin B (7), asperfumigatin (8), spirocyclic diketopiperazine alkaloid (9), fumigaclavine C (10), monomethylsulochrin (11), thymidine (12), fumigaclavine A (13), chaetominine (14), prenylcyclotryprostatin A (15), fumiquinazoline J (16). Compound 15 was first discovered from the genus Aspergillus. Compound 2 exhibited potent antibacterial activity against Listeria monocytogenes, with a minimum inhibitory concentration (MIC) of 4.03 μg·mL -1. Compound 3, 4 and11 demonstrated moderate antibacterial activity against L. monocytogenes, with MIC values of 64.54 μg·mL -1, 66.25 μg·mL -1, and 78.63 μg·mL -1, respectively. Compound 1 displayed strong DPPH radical scavenging activity. Furthermore, compounds 1, 10, 11, and 13 exhibited potent ABTS radical scavenging activity.

Key words: Aspergillus fumigatus, secondary metabolites, antibacterial activity, antioxidant activity