海草床与红树林、珊瑚礁并称为地球上三大典型的近海海洋生态系统，具有极高的生态服务功能，被誉为“海洋之肺”。海草床具有较高的初级生产力水平，在维持海洋生态系统的平衡与稳定方面发挥着核心作用。海草根际的真核微生物对海草的生长发育至关重要，在维持海草健康和生态系统碳、氮等营养元素循环过程中发挥重要作用。本研究利用高通量测序技术分析了山东沿海地区中国川蔓草(Ruppia sinensis)、鳗草(Zostera marina)和日本鳗草(Zostera japonica)根际真核微生物的多样性特征、群落结构及其与环境因子的关系。/t/n和环节动物门(Annelida)。海草根际真核微生物群落结构与根际沉积物的总碳含量、亚硝酸盐含量、铵盐含量和沉积物粒径大小显著相关。共现性网络分析揭示，日本鳗草根际真核微生物之间的互作关系更加复杂。本研究阐明了海草根际真核微生物的群落结构及其影响因子，为进一步探讨海草床生态系统物质循环及能量流动奠定理论基础。

Seagrass beds, mangroves, and coral reefs are known as the three typical offshore marine ecosystems on earth. Seagrass beds, which possessing extremely high ecological service functions and are hailed as the "lungs of the ocean". Seagrasses have a relatively high level of primary productivity and play a core role in maintaining the balance and stability of the marine ecosystem. The rhizosphere eukaryotic microorganisms are crucial for seagrass growth and development, playing a crucial role in sustaining seagrass health and facilitating the cycling of nutrients such as carbon and nitrogen within the ecosystem. In this study, high - throughput sequencing technology was employed to investigate the community structure and diversity of eukaryotic microorganisms in the rhizosphere of Ruppia sinensis, Zostera marina, and Zostera japonica along the coastal areas of Shandong Province. The results showed that eukaryotic microorganisms associated with seagrasses were mainly composed of algae, metazoans, and protozoa, with Chlorophyta and Annelida being the dominant groups. The community structure of eukaryotic microorganisms in the seagrass’s rhizosphere was significantly correlated with the total carbon content, nitrite content, ammonium content, and sediment particle size. Co-occurrence network analysis further revealed that the interactions among eukaryotic microorganisms in Zostera japonica were more complex. This study elucidates the community structure and influencing factors of eukaryotic microorganisms in the seagrass rhizosphere, which laying a theoretical foundation for further exploration of the nutrients cycle and energy flow in seagrass ecosystem.