收稿日期: 2009-12-04
修回日期: 2010-02-04
网络出版日期: 2010-07-29
基金资助
广东省科技计划项目(2008A020800012); 现代渔业产业技术体系建设项目(nycytx-49); 国家“863”项目 (2007AA10Z239); 珠
海市科技项目(c20082031)
Effects of acute salinity stress on Na+/K+-ATPase activity and plasma indicators of Epinephelus coioides
Received date: 2009-12-04
Revised date: 2010-02-04
Online published: 2010-07-29
Supported by
广东省科技计划项目(2008A020800012); 现代渔业产业技术体系建设项目(nycytx-49); 国家“863”项目 (2007AA10Z239); 珠
海市科技项目(c20082031)
研究了急性盐度胁迫对斜带石斑鱼幼鱼Epinephelus coioides鳃丝Na+/K+-ATP酶活性和血清应激指标的影响, 将养殖于自然海水(盐度34‰)中, 体重为(19.59±0.25) g的斜带石斑鱼幼鱼直接转移至盐度24‰、14‰、4‰和0‰的水体中, 于转移后1、3、6、12和24h分别检测鳃丝Na+/K+-ATP酶活性和血清中血糖、天门冬氨酸氨基转移酶(AST)、溶菌酶的变化。试验表明: 试验组Na+/K+-ATP酶活性变化基本一致, 均在1h时达到最高值, 随后下降, 至6h达到稳定且均显著高于对照组(P<0.05); 血糖在24‰和14‰盐度组呈下降趋势, 在4‰和0‰盐度组3h时出现最低值, 在6h时达到峰值, 随后逐渐下降; AST水平在24‰和14‰盐度组与对照组无显著差异(P>0.05), 在4‰和0‰盐度组均呈现先上升后下降的趋势, 于6和12h时达到各自峰值; 溶菌酶含量在试验24h时, 在24‰、14‰和4‰盐度组间差异显著(P<0.05), 在0‰盐度组呈现先上升后下降的趋势, 至6h时达到峰值。试验显示, 斜带石斑鱼幼鱼由盐度34‰的水体转移至盐度24‰和14‰的水体后, 其应激强度较弱; 由盐度34‰的水体转移至盐度4‰和0‰的水体后, 其应激反应较大, 适应盐度变化需时也较长。根据本试验结果, 在对斜带石斑鱼进行应激性淡化转运时, 可将其直接从34‰高盐度自然海水中转移至14‰盐度的水体后, 再缓慢降至预定盐度, 从而减少淡化时间。
关键词： 斜带石斑鱼Epinephelus coioides; 盐度胁迫; Na+/K+ -ATP酶; 血糖; 天门冬氨酸氨基转移酶; 溶菌酶
杨宇晴,余德光,谢骏,郁二蒙,李望东,王广军,王海英,龚望宝 . 急性盐度胁迫对斜带石斑鱼Na+/K+-ATP酶及血清应激指标的影响[J]. 热带海洋学报, 2010 , 29(4) : 160 -164 . DOI: 10.11978/j.issn.1009-5470.2010.04.160
Effects of acute salinity stress on plasma indicators and Na+/K+-ATPase activity in gills of Epinphelus coioides juveniles were studied. E. coioides juveniles with body weight (19.59 ±0.25)g in the seawater with salinity of 34‰ were directly transferred to water with salinities at 0‰, 4‰, 14‰, and 24‰, while those in seawater with salinity of 34‰ were taken as the control group. Plasma glucose content, activities of lysozyme, aspartate carbamyl transferase (AST) and Na+/K+-ATPase in gills were determined at 1, 3, 6, 12, and 24h after the transferring of E. coioides. The results showed that Na+/K+-ATPase activity in salinity stress groups displayed a similar trend of increasing first to its peak at 1h, decreasing thereafter, remaining stable at 6h, and being significantly higher than that in the control group later (P<0.05). Plasma glucose content in waters with salinities 24‰ and 14‰ showed a downward trend, and the content in waters with salinities 4‰ and 0‰ exhibited the lowest value at 3h, followed by an upward trend with the peak occurring at 6h and a gradual decrease thereafter. There were no significant differences in AST among waters with salinity of 14‰, 24‰ and 34‰ (P>0.05). AST in waters with salinities 4‰ and 0‰ reached its peak at 6h and 12h, respective, and then decreased. There were significant differences in lysozyme activity among waters with salinity of 24‰, 14‰ and 4‰ at 24h (P<0.05). Lysozyme activity in salinity 0‰ increased first and then declined, with the peak occurring at 6h. In conclusion, E. coioides juveniles had a strong adaptability to ambient salinity stress, and they could adapt themselves at 6h. In waters with salinity at 14‰ and 24‰, E. coioides juveniles showed the weakest response. According to the results of this study, we conclude that E. coioides juveniles can be transferred directly from seawater to water with salinity of 14‰ before the salinity in water is gradually reduced to a prospective salinity value, which can reduce the time of desalination.
Key words： Epinephelus coioides; salinity stress; Na+/K+ -ATP; plasma glucose; AST; lysozyme
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