收稿日期: 2009-03-24
修回日期: 2009-08-26
网络出版日期: 2010-12-15
基金资助
中国科学院南海海洋所研究所前沿领域青年项目(SQ200815); 中国科学院、国家外国专家局创新团队国际合作伙伴计划
Effects of N to P ratio on the growth of two red tide diatom Skeletonema costatum and Thalassiosira weissflogii
Received date: 2009-03-24
Revised date: 2009-08-26
Online published: 2010-12-15
Supported by
中国科学院南海海洋所研究所前沿领域青年项目(SQ200815); 中国科学院、国家外国专家局创新团队国际合作伙伴计划
实验室条件下用不同氮磷摩尔比(4︰1, 16︰1, 64︰1)的培养液培养中肋骨条藻Skeletonema costatum和威氏海链藻Thalassiosira weissflogii, 对它们的比生长率、细胞状态、细胞对外界氮磷营养元素的吸收和细胞内氮磷比的变化进行了研究。结果表明, 氮磷比显著影响两种硅藻的生长和生理状态, 氮浓度对细胞生长的影响更大。N限制组(N︰P=4︰1)的比生长率、细胞数量和叶绿素a含量明显低于正常条件和P限制组(N︰P=64︰1); 威氏海链藻生长对N的变化比中肋骨条藻更为敏感, 吸收外界无机氮的速率更快。营养盐充足的情况下, 水体中藻细胞的氮磷比变化会较小, 但由于“奢侈消费”现象的存在, 在出现营养盐限制时, 细胞的氮磷比组成会跟随环境的氮磷比改变, 在氮限制的条件下, 细胞的氮磷比会相应减少, 而相反在磷限制的条件下, 细胞的氮磷比会明显增加。
刘皓,高永利,殷克东,袁翔城,徐杰,HARRISON Paul J . 不同氮磷比对中肋骨条藻和威氏海链藻生长特性的影响[J]. 热带海洋学报, 2010 , 29(6) : 92 -97 . DOI: 10.11978/j.issn.1009-5470.2010.06.092
The growth character and ecophysiology of Skeletonema costatum and Thalassiosira weissflogii were studied in culture medium of different ambient ratios of N to P (4︰1, 16︰1, 64︰1). Bacth cultures of these two phytoplankton species were conducted to examine their growth rates, Chl-a, DIN︰P (dissolved inorganic nitrogen and phosphorus) and PON︰P (particular organic nitrogen and phosphorus) during incubation periods. The results showed that the N︰P ratio could signifi-cantly influence the growth of Skeletonema costatum and Thalassiosira weissflogii. The ratio of N to P could evidently influ-ence these two diatom species. The growth rate, cell numbers and Chl-a of these two species were obviously lower with N-limitation (N︰P=4︰1) than those with P-limitation (N︰P=64︰1). The results suggested that T. weissflogii was more sensitive to N-limitation than S. costatum. Although cultured in different N︰P ratios medium, the N︰P composition of these two algal species hardly changed with the medium N︰P ratio. Due to the luxury consumption, the cells could still take up excessive nutrients for living when other nutrients were depleted by phytoplankton, which resulted in different N︰P ratios of phytoplankton. N-limited medium reduced the N︰P ratio of the algal cells, while P-limited medium increased the N︰P ratio of the phytoplankton cells significantly.
[1] REDFIELD A C. The biological control of chemical fators in the environment [J]. Am.Sci., 1958, 46: 205-222.
[2] 袁美玲, 王朝晖, 李友富. N、P营养盐对海洋卡盾藻(Chattonella marina)生长的影响. [J] 生态学报, 2008, 28(1): 0430-0435.
[3] 刘东艳, 孙军, 陈宗涛, 等. 不同氮磷比对中肋骨条藻生长特性的影响. [J] 海洋湖沼通报, 2002, (2): 39-44.
[4] 李铁, 史致丽, 李俊, 等, 营养盐对中肋骨条藻和新月菱形藻部分生化组成和性质的影响. [J] 海洋与湖沼, 2000, 31(3): 239-244.
[5] 李顺兴, 郑凤英, 洪华生, 等, 氮磷营养盐对微氏海链藻细胞生化组成的影响. [J] 海洋科学, 2006, 30(1): 49-53.
[6] FLYNN J K. How critical is the critical N︰P ratio? [J]. Phycol., 2002, 38: 961-970.
[7] MAGUER J F, WAFAR M, and Madec C, et al. Nitrogen and phosphorus requirements of an Alexandrium minutum bloom in the Penzi estuary, France[J]. Limnol. Oceanogr., 2004, 49: 1108-1114.
[8] HODGKISS I J, LU S H. The effects of nutrients and their ratios on phytoplankton abundance in
[9] DORTSCH Q, CLAYTON J J R, Thoresen S S. Species Differences in Accumulation of Nitrogen Pools in Phytoplankton [J]. Mar Biol, 1984, 81: 237-250.
[10] THOMPSON P A, OH H M, RHEE G Y. Storage of Phosphorus in Nitrogen-fixing Anabaena flos-aquae (Cyanophyceae) [J]. J Phycol, 1994, 30: 267-273.
[11] KILHAM P, HECKY R E. Comparative Ecology of Marine and Freshwater Phytoplankton [J]. Limnol Oceanogr, 1988, 33(4, Part 2): 776-795.
[12] 高学庆, 任久长, 宗志祥, 等. 铜绿微囊藻营养动力学研究[J]. 北京大学学报：自然科学版, 1994, 30(4): 461-469.
[13] JIA X P, CAI W G, LIN Q. The effects of major pollutions on mariculture and fishery resource enhancemenr alone the coastal waters of China[J]. Journal of Fishery Science of China, 1997, 4(4): 78-81.
[14] GUILLARD R L, RYTHER J H. Studies on marine planktonic diatoms: I Cyclotella nana (Hustedt) and Detomdaconfervacea (Cleve) [J]. Can J Microbiol, 1962, 8: 229-239.
[15] BERGES J A, FRANKLIN D J. Evolution of an artificial seawater medium: improvements in enriched seawater, artificial water over the last two decades. [J] J Phycol, 2001, 37: 1138-1145.
[16] Sun J, Ning X R. Marine phytoplankton specific growth rate[J]. Advances in Earth Science, 2005, 20(9): 939-945.
[17] PARSONS T R, MAITA Y, AND LALLI C M. A manual of chermical and biological methods for seawater analysis[M].
[18] KOROLEFF F. Determination of total and organic phosphorus by alkaline persulphate oxidation[M]//Methods for seawater analysis. 3rd ed.
[19] KNAP A, MICHAELS A, CLOSE A, et al . Protocols for the Joint Global Ocean Flux Study(JGOFS) core measurements[R]//JGOFS report No 19. Reprint of the IOC Manual and Guides No 29, 1994, UNESCO, Paris.
[20] BRAND L E, GUILLARD R R, MURPHY L S. A method for the rapid and precise determination of acclimated phytoplankton reproduction rates[J]. J Plankton Res.1981, 3: 193-414.
[21] 郝建华, 霍文毅, 俞志明. 胶州湾增养殖海域营养状况与赤潮形成的初步研究[J]. 海洋科学, 2000, 20(4): 37-41.
[22] KETCHUM B H. The Absorption of Phosphate and Nitrate by Illuminated Cultures of Nitzschia closterium[J]. Am J Bot, 1939, 26: 399-407.
[23] SUNDA W G, SUSAN A H. Effect of CO2 Supply and demand on zinc uptake and growth limitation in a coastal diatom[J]. Limnol Oceanogr, 2005, 50(4): 1181-1192.
[24] KUSTKA A, SERGIO S, CARPENTER E J, et al. a revised extimate of the iron use efficiency of nitrogen fixation, with special reference to the marine Cyanobacterium Trichodesmium sp. (Cyanophyta)[J]. J Phycol, 2003, 39: 12-25.
[25] DORTSCH Q, CLAYTON J J R, Thoresen S S. Species differences in accumulation of nitrogen pools in phytoplankton[J]. Mar Biol, 1984, 81: 237-250.
[26] THOMPSON P A, OH H M, RHEE G Y. Storage of phosphorus in nitrogen-fixing anabaena flos-aquae (Cyanophyceae) [J]. J Phycol, 1994, 30: 267-273.
[27] SCOOT G L, SUSAN S K, DANIEL A K, et al. Effect of nutrient availability on the biochemical and elemental stoichiometry in the freshwater diatom Stephanodiscus minutulus (Bacillariophyceae)[J]. J Phycol, 2000, 36: 510-512.
[28] GEIDER R J, ROCHE J L. Redfield revisited: Variability of C:N:P in marine microalgae and Its biochemical basis[J]. Eur J Phycol, 2002, 37: 1-17.
[29] ANDERSEN T, SCHARTAU A K L, PAASCHE E. Quantifying external and internal nitrogen and phosphorus pools as well as nitrogen and phosphorus supplied through remineralization, in coastal marine plankton by means of a dilution technique[J]. Mar Ecol Prog Ser, 1991, 69: 67-80.
[30] 孙军, 刘东艳, 陈宗涛, 等. 不同氮磷比率对青岛大扁藻、新月柱鞘藻和米氏凯伦藻生长影响及其生存策略研究[J]. 应用生态学报, 2004, 15(11): 2122-2126.
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