1. 中国水产科学研究院南海水产研究所, 广东 广州 510300; 2. 上海海洋大学水产与生命学院, 上海 201306
收稿日期: 2010-05-10
修回日期: 2010-06-29
网络出版日期: 2011-07-20
基金资助
国家高技术研究发展计划重点项目(2006AA10A414); 中央级公益性科研院所基本科研业务费专项资金项目(2008YD02)
Genetic polymorphism of three cultured populations of golden pompano Trachinotus ovatus as revealed by microsatellites
1. South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Received date: 2010-05-10
Revised date: 2010-06-29
Online published: 2011-07-20
运用 6 对微卫星引物分别对海南、深圳、福建 3 鲹 个地区的卵形鲳 Trachinotus ovatus 养殖群体的遗传差异
进行分析。结果表明, 3 个养殖群体的平均等位基因数(Na)为 3.67—3.83, 平均有效等位基因数(Ne)为 2.43—3.03,
平均观测杂合度(Ho)为 0.48—0.66, 平均期望杂合度(He)为 0.56—0.64, 平均多态信息含量(PIC)为 0.49—0.55, 可
见 3 个养殖群体的遗传多样性较高。 Hardy-weinberg 平衡遗传偏离指数(d)显示海南和深圳养殖群体杂合子过剩(分
别为 0.10 和 0.12), 而福建养殖群体则出现杂合子缺失(−0.08)。统计检验发现 3 个群体中各位点的遗传偏离并不
显著(p＞0.05)。3 个养殖群体的遗传相似度及遗传距离信息聚类分析结果表明, 福建与深圳养殖群体的亲缘关系
较近, 两者与海南养殖群体的亲缘关系较远。3 个养殖群体的确切来源还有待进一步研究。
吉磊 ,区又君 ,李加儿 . 卵形鲳鲹 3 个养殖群体的微卫星多态性分析[J]. 热带海洋学报, 2011 , 30(3) : 62 -68 . DOI: 10.11978/j.issn.1009-5470.2011.03.062
Amplifications with six pairs of microsatellite primers were used to determine the genetic polymorphism of genomic DNA in three cultured populations of golden pompano Trachinotus ovatus, which were collected in Hainan, Shenzheng, and Fujian provinces. The data were analyzed by software package PopGen ver. 32, and their phylogenetic trees were constructed using Mega ver. 4.0. The results show that the value of average number of alleles (Na) was 3.67-3.83 in all three populations; the value of average effective number of alleles (Ne) was 2.43-3.03; the observed value of mean heterozygosity (Ho) varied from 48% to 66%; the expected value of mean heterozygosity (He) varied from 56% to 64%; and the value of average polymorphism information content (PIC) was 0.49-0.55. A high genetic diversity among the three populations could be concluded from the data collected. In addition, we computed the Hardy-Weinberg index to access the distribution of genotypes, and the values in Hainan, Shenzhen, and Fujian populations were 0.10, 0.12, and -0.08, respectively. The Bonferroni Correction test showed that there is no significant genetic disequilibrium among the three populations (p＞0.05). Cluster analysis was also carried out based on the Nei's genetic identity and genetic distance. The results reveal that phylogenetic relationship between the populations in Fujian and Shenzhen was closer, and the origins of these populations may request further studies.
[1] TUTMAN P, GLAVI? N, KO?UL V, et al. Preliminary information on feeding and growth of pompano, Trachinotus ovatus (Linnaeus 1758) (Pisces; Carangidae) in captivity[J]. Aquaculture International, 2004, 12(4/5): 387-393.
[2] 陶启友, 郭根喜. 卵形鲳鲹深水网箱养殖试验[J]. 科学养鱼, 2006(2): 39.
[3] 陶启友, 郭根喜. 深水网箱养殖卵形鲳鲹应注意的几个问题[J]. 齐鲁渔业, 2006, 23(9): 26-27.
[4] 唐志坚, 张璐, 马学坤. 卵形鲳鲹和南美白对虾池塘混养技术[J]. 内陆水产, 2008(9): 24-25.
[5] 罗杰, 杜涛. 卵形鲳鲹不同养殖方式的研究[J]. 水利渔业, 2008, 28(1): 70-71.
[6] 区又君, 李加儿. 卵形鲳鲹的早期胚胎发育[J]. 中国水产科学, 2005, 12(6): 786-789.
[7] 李加儿, 区又君, 刘匆. 红笛绸和卵形鲳鲹鳃的扫描电镜观察与功能探讨[J]. 海洋水产研究, 2007, 28(6): 45-50.
[8] 齐旭东, 区又君. 卵形鲳鲹不同组织同工酶表达的差异[J].南方水产, 2008, 4(3): 38-42.
[9] 王瑞旋, 刘广锋, 王江勇, 等. 养殖卵形鲳鲹诺卡氏菌病的研究[J]. 海洋湖沼通报, 2010, 7(1): 52-58.
[10] 王江勇, 郭志勋, 黄剑南, 等. 一起卵形鲳鲹幼鱼死亡原因的调查[J]. 南方水产, 2006, 2(3): 54-56.
[11] XU HAIDONG, FENG JUAN, GUO ZHIXUN, et al. Detection of red-spotted grouper nervous necrosis virus by loop-mediated isothermal amplification[J]. Journal of Virological Methods, 2010, 163(1): 123-128.
[12] LI GUIFENG, ZHAO DIANHUI, HUANG LU, et al. Identification and phylogenetic analysis of Vibrio vulnificus isolated from diseased Trachinotus ovatus in cage mariculture[J]. Aquaculture, 2006, 261(1): 17-25.
[13] SPARKS J S, DUNLAP P V, SMITH W L. Evolution and diversification of a sexually dimorphic luminescent system in ponyfishes (Teleostei: Leiognathidae), including diagnoses for two new genera[J]. Cladistics, 2005, 21(4): 305-327.
[14] CHEN W L, BONILLO C, LECOINTRE G. Repeatability of clades as a criterion of reliability: a case study for molecular phylogeny of Acanthomorpha (Teleostei) with larger number of taxa[J]. Molecular Phylogenetics and Evolution, 2003, 26: 262-288.
[15] 孙效文, 张晓峰, 赵莹莹, 等. 水产生物微卫星标记技术研究进展及其应用[J]. 中国水产科学, 2008, 15(4): 689-703.
[16] LI DAYU, KANG DAHAI, YIN QIANQIAN, et al. Microsatellite DNA marker analysis of genetic diversity in wild common carp(Cyprinus carpio L.) populations[J]. Genetics and Genomics, 2007, 34(11): 984-993.
[17] 尹邵武, 廖经球, 黄海, 等. 海南近海点带石斑鱼野生和养殖群体微卫星多态分析[J]. 应用与环境生物学报, 2008, 14(2): 215-219.
[18] HUANG B X, PEAKALL R, HANNA P J. Analysis of genetic structure of blacklip abalone (Haliotis rubra) populations using RAPD, minisatellite and microsatellite markers[J]. Mar Biol, 2000, 136(2): 207-216.
[19] 张研, 梁利群, 常玉梅, 等. 鲤鱼体长性状的QTL定位及其遗传效应分析[J]. 遗传, 2007, 29(10): 1243-1248.
[20] JACKSON T R, FERGUSON M M, DANZMANN R G, et al. Identification of two QTL influencing upper temperature tolerance in three rainbow trout half-sib families[J]. Heredity, 1998, 80(2): 143-151.
[21] NIELSEN E E, HANSEN M M. Looking for a needle in a haystack: discovery of indigenous Atlantic salmon (Salmo salar L.) in stocked population[J]. Conservation Genetics, 2001, 2(3): 219-232.
[22] 宋红梅, 白俊杰, 全迎春, 等. 三种罗非鱼的微卫星分子鉴定和遗传结构分析[J].农业生物技术学报, 2008, 16(6): 952-958.
[23] NICHOL K M, YOUNG W P, DANZMANN R G. A consolidated linkage map for rainbow trout[J]. Animal Genetics, 2003, 34(2): 102-115.
[24] KOCHER T D, LEE W J, SOBOLEWSKA H, et al. A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus)[J]. Genetics, 1998, 148(3): 1225-1232.
[25] CÁRDENAS L, SILVA A X, MAGOULAS A, et al. Genetic population structure in the Chilean jack mackerel, Trachurus murphyi (Nichols) across the South-eastern Pacific Ocean[J]. Fisheries Research, 2009, 2(100): 109-115.
[26] GONG PING, LI JIALE, YUE GENHUA. Twelve novel microsatellite loci from an endangered marine fish species golden pompano Trachinotus blochii[J]. Conservation Genetics, 2009, 10(5): 1365-1367.
[27] SAMBROOK J, RUSSELL D W. Molecular cloning: A Laboratory Manual[M]. 3 rd ed. New York: Cold spring Harbor Laboratory Press, 2001: 17.
[28] NEI M. Analysis of gene diversity in subdivided populations [J]. Proc Natl Acad Sci USA, 1973, 70(12): 3321-3323.
[29] 全迎春, 梁利群, 孙效文, 等. 斑马鱼微卫星分子标记检测鲤鱼种间多态性[J]. 中国水产科学, 2006, 13(2): 300-304.
[30] 郭昱嵩, 王中铎, 刘楚吾, 等. 9 种常见笛鲷微卫星位点筛选与遗传多样性分析[J]. 热带海洋学报, 2010, 29(3): 82-86.
[31] ULRIKE S. Characterization and cross-species amplification of microsatellite loci in a Cyprinodon species flock[J]. Molecular Ecology Notes, 2006, 6(3): 843-846.
[32] MCQUOWN E C, SLOSS B L, SHEEHAN R J, et al. Microsatellite analysis of genetic variation in sturgeon (Acipenseridae): new primer sequences for Scaphirhynchus and Acipenser[J]. Trans Am Fish Soc, 2000, 129(6): 1380-1388.
[33] BOTSTEIN D, WHITE R L, SKOLNICK M, et al. Construction of a genetic linkage map in man using restriction fragment length polymophisms[J]. Am J Hum Genet, 1980, 32(3): 314-331.
[34] YUE G H, ZHU Z Y, LIN G, et al. Novel polymorphic microsatellites for studying genetic diversity of red Asian arowanas[J]. Conservation Genetics, 2006, 7(4): 627-629.
[35] 李先仁, 李思发, 唐首杰, 等. 尼罗罗非鱼8个养殖群体遗传差异的微卫星分析[J]. 上海海洋大学学报, 2009, 18(1): 1-7.
[36] FALCONE D S, MACKAY T F C. Introduction to Quantitative Genetics[M]. 4 th ed. UK: Addison Wesley Longman Limited, 1996:19-27.
[37] 杜博, 龚世园, 童馨, 等. 皱纹盘鲍和盘鲍南方养殖群体遗传变异的微卫星分析[J]. 南方水产, 2007, 6(3): 22-29.
[38] 刘丽, 刘楚吾, 郭昱嵩, 等. 青石斑鱼微卫星DNA标记的筛选及群体遗传多样性分析[J]. 中国水产科学, 2008, 15(1): 22-29.
[39] 蒋家金, 李瑞伟, 叶富良. 罗非鱼4 个选育群体遗传结构SSR分析[J]. 广东海洋大学学报, 2008, 28(4): 10-14.
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