热带海洋学报 ›› 2011, Vol. 30 ›› Issue (3): 62-68.doi: 10.11978/j.issn.1009-5470.2011.03.062cstr: 32234.14.j.issn.1009-5470.2011.03.062

• 海洋生物学 • 上一篇    下一篇

卵形鲳鲹 3 个养殖群体的微卫星多态性分析

吉磊1,2, 区又君1,李加儿1   

  1. 1. 中国水产科学研究院南海水产研究所, 广东 广州 510300; 2. 上海海洋大学水产与生命学院, 上海 201306

  • 收稿日期:2010-05-10 修回日期:2010-06-29 出版日期:2011-07-18 发布日期:2011-07-20
  • 作者简介:吉磊(1985—), 男, 湖南省浏阳市人, 硕士研究生, 主要从事鱼类育种研究。E-mail: jous911@126.com
  • 基金资助:

    国家高技术研究发展计划重点项目(2006AA10A414); 中央级公益性科研院所基本科研业务费专项资金项目(2008YD02)

Genetic polymorphism of three cultured populations of  golden pompano Trachinotus ovatus as revealed by microsatellites

JI Lei1, 2, OU You-jun1, LI Jia-er1   

  1. 1. South China Sea Fisheries Research Institute, CAFS, Guangzhou 510300, China; 2. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • Received:2010-05-10 Revised:2010-06-29 Online:2011-07-18 Published:2011-07-20
  • Contact: 区又君。E-mail: ouyoujun@126.com

摘要:

运用 6 对微卫星引物分别对海南、深圳、福建 3 鲹 个地区的卵形鲳 Trachinotus ovatus 养殖群体的遗传差异
进行分析。结果表明,  3 个养殖群体的平均等位基因数(Na)为 3.67—3.83,  平均有效等位基因数(Ne)为 2.43—3.03,
平均观测杂合度(Ho)为 0.48—0.66,  平均期望杂合度(He)为 0.56—0.64,  平均多态信息含量(PIC)为 0.49—0.55,  可
见 3 个养殖群体的遗传多样性较高。 Hardy-weinberg 平衡遗传偏离指数(d)显示海南和深圳养殖群体杂合子过剩(分
别为 0.10 和 0.12),  而福建养殖群体则出现杂合子缺失(−0.08)。统计检验发现 3 个群体中各位点的遗传偏离并不
显著(p>0.05)。3 个养殖群体的遗传相似度及遗传距离信息聚类分析结果表明,  福建与深圳养殖群体的亲缘关系
较近,  两者与海南养殖群体的亲缘关系较远。3 个养殖群体的确切来源还有待进一步研究。

关键词: 卵形鲳 ,  , 养殖群体,  , 微卫星 DNA,  , 遗传多样性

Abstract:

Amplifications with six pairs of microsatellite primers were used to determine the genetic polymorphism of genomic DNA in three cultured populations of golden pompano Trachinotus ovatus, which were collected in Hainan, Shenzheng, and Fujian provinces. The data were analyzed by software package PopGen ver. 32, and their phylogenetic trees were constructed using Mega ver. 4.0. The results show that the value of average number of alleles (Na) was 3.67-3.83 in all three populations; the value of average effective number of alleles (Ne) was 2.43-3.03; the observed value of mean heterozygosity (Ho) varied from 48% to 66%; the expected value of mean heterozygosity (He) varied from 56% to 64%; and the value of average polymorphism information content (PIC) was 0.49-0.55. A high genetic diversity among the three populations could be concluded from the data collected. In addition, we computed the Hardy-Weinberg index to access the distribution of genotypes, and the values in Hainan, Shenzhen, and Fujian populations were 0.10, 0.12, and -0.08, respectively. The Bonferroni Correction test showed that there is no significant genetic disequilibrium among the three populations (p0.05). Cluster analysis was also carried out based on the Nei's genetic identity and genetic distance. The results reveal that phylogenetic relationship between the populations in Fujian and Shenzhen was closer, and the origins of these populations may request further studies.

Key words: Trachinotus ovatus , cultured population , microsatellite DNA , genetic diversity