海洋环境保护

不同环境条件下斑节对虾家系生长和免疫酶活性差异

  • 杨其彬 ,
  • 王专伟 ,
  • 周发林 ,
  • 温为庚 ,
  • 苏天凤 ,
  • 邱丽华 ,
  • 黄建华
展开
  • 1. 中国水产科学研究院南海水产研究所, 农业部南海渔业资源开发利用重点试验室, 广东 广州 510300; 2. 上海海洋大学, 上海 201306
杨其彬(1972—), 男, 广东省高州市人, 助理研究员, 主要从事水产动物繁育与遗传育种研究。E-mail: yangqibin1208@163.com

收稿日期: 2011-12-22

  修回日期: 2012-02-29

  网络出版日期: 2013-08-28

Analysis on growth and characteristic of immune enzymes activity of Penaeus monodon family under environment-stress

  • YANG Qi-bin ,
  • WANG Zhuan-wei ,
  • ZHOU Fa-lin ,
  • WEN Wei-geng ,
  • SU Tian-feng ,
  • QIU Li-hua ,
  • HUANG Jian-hua
Expand
  • 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Utilization, Ministry of Agriculture, Guangzhou 510300, China; 2. Shanghai Ocean University, Shanghai 201306, China

Received date: 2011-12-22

  Revised date: 2012-02-29

  Online published: 2013-08-28

摘要

研究了正常生长、低温胁迫和高氨氮胁迫下10个斑节对虾家系生长特性及超氧化物歧化酶(Superoxide Dismutase, SOD)、酸性磷酸酶(Acid Phosphatase, ACP)、酚氧化酶(Phenoloxidase, PO)3种免疫酶活性的变化。生长试验结果表明, 10个家系特定生长率(SGR)呈现明显差异, 家系077与家系017和661的ACP活性存在极显著差异, 家系4612与家系017的SOD活性存在极显著性差异, 而其他家系的免疫酶活性无显著差异。斑节对虾在48h低温胁迫下, ACP和SOD活性先升高后降低, PO活性则不断降低, 而对照组的3种酶活性呈无规律轻微变化。斑节对虾在48h高氨氮胁迫下, ACP活性不断升高, SOD在0—8h活性上升, 8—48h活性快速降低, PO活性不断下降, 而对照组的3种酶活性呈无规律轻微变化;一些家系间的3种免疫酶活性存在显著性差异, 其中家系4612的3种酶活性均高于其他家系。3个试验中, 家系的特定生长率与免疫酶活性均呈负相关。

本文引用格式

杨其彬 , 王专伟 , 周发林 , 温为庚 , 苏天凤 , 邱丽华 , 黄建华 . 不同环境条件下斑节对虾家系生长和免疫酶活性差异[J]. 热带海洋学报, 2013 , 32(3) : 78 -85 . DOI: 10.11978/j.issn.1009-5470.2013.03.012

Abstract

The response of Superoxide Dismutase (SOD), Acid Phosphatase (ACP), and Phenoloxidase (PO) in Penaeus monodonfamilies to normal growth, low temperature stress and high ammonia stress was studied under laboratory condition. Growth in terms of specific growth rate (SGR) was significant difference among the selected families (p<0.05). The activities of ACP were significant difference among family 077, 017 and 661. The activities of SOD were significant difference between family 4612 and family 017. When the rearing temperature decreased from 27 ℃ to 20 ℃, the activities of ACP and SOD first increased and then decreased, the activities of PO reduced prolonged according to the low temperature stress, and the minor changes of control trial were not regular. Under the high ammonia stress, the activities of ACP increased prolonged, the activities of SOD increased in 0-8 h, then rapidly decreased in 8-48 h, the activities of PO reduced in 0-48 h, and the minor changes of control trial were not regular. The activities of immune enzymes of some families were significant differences, and three immune enzymes activities of family 4612 higher than that of other families under the high ammonia stress. A negative correlation was found between the specific growth rate and the activities of ACP, SOD and PO of ten families in three trials.

参考文献

[1] LE MOULLAC G, HAFFNER P. Environmental factors affecting immune responses in Crustacea[J]. Aquaculture, 2000, 191(1-3): 121-131.
[2] CHENG W, CHEN J C. Effects of intrinsic and extrinsic factors on the haemocytes profiles of the prawn, Macrobrachium rosenbergii[J]. Fish Shellfish Immunol, 2001, 11(1): 53-63.
[3] GOMEZ-JIMENEZ S, UGLOW R F, GOLLAS-GALVAN T. The effects of cooling and emersion on total haemocyte count and phenoloxidase activity of the spiny lobster Paulitrus interruptus[J]. Fish Shellfish Immunol, 2000, 10(7): 631-635.
[4] STEENBERGEN J F, STEENBERGEN S M, SCHAPIRO H C. Effects of temperature on phagocytosis in Homarus americanus[J]. Aquaculture, 1978, 14(1): 23-30.
[5] VARGAS-ALBORES F, HINOJOSA-BALTAZAR P, POTTILLO-CLARK G, et al. Influence of temperature and salinity on the yellowlegs shrimp Penaeus californiensis Holmes, prophenoloxidase system[J]. Aquac Res, 1998, 29(8): 549-553.
[6] 孙舰军, 丁美丽. 氨氮对中国对虾抗病力的影响[J]. 海洋与湖沼, 1999, 30(3): 267-272.
[7] 王克行. 虾蟹类增养殖学[M]. 北京: 中国农业出版社, 1997: 91, 218.
[8] ARMSTRONG D A, CHIPPENDALE D, KNIGHT A W, et al. Interaction of ionized and un-ionized ammonia on short- term survival and growth of prawn larvae, Macrobrachium rosenbergii[J]. Biol Bull, 1978, 154(1): 15-31.
[9] CHENG W, CHEN J C. The virulence of Enterococcus to freshwater prawn Macrobrachium rosenbergii and its immune resistance under ammonia stress[J]. Fish Shellfish Immunol, 2002, 12(2): 97-109.
[10] 王兴强, 马甡, 董双林. 盐度和蛋白质水平对凡纳滨对虾存活、生长和能量转换的影响[J]. 中国海洋大学学报: 自然科学版, 2005, 35 (1): 33-37.
[11] 潘鲁青, 姜令绪. 盐度和pH突变对2种养殖对虾免疫力的影响[J]. 中国海洋大学学报: 自然科学版, 2002, 32 (6): 903-910.
[12] 房文红, 王慧, 来琪芳. 碳酸盐碱度、pH对中国对虾幼虾的致毒效应[J]. 中国水产科学, 2001, 7 (4) : 78-81.
[13] 杨富亿, 李秀军, 杨欣乔. 日本沼虾幼虾对碱度和pH的适应性[J]. 动物学杂志, 2005, 40 (6): 74-79.
[14] 沈文英, 胡洪国, 潘雅娟. 温度和pH值对南美白对虾(Penaeus vannmei)消化酶活性的影响[J]. 海洋与湖沼, 2004, 35 (6): 543-548.
[15] 王专伟. 斑节对虾家系生长、抗病、抗逆性状与免疫酶活性的相关分析[D]. 上海: 上海海洋大学, 2011:38-49.
[16] 杨其彬, 温为庚, 黄建华, 等. 斑节对虾4个不同地理群体建立家系的生长及成活[J]. 南方水产, 2010, 6(3): 36-40.
[17] 黄忠, 林黑着, 黄建华, 等. 斑节对虾6个家系生长、饲料利用和全虾营养成分的比较[J]. 南方水产, 2009, 5 (1): 42-47.
[18] HETZEL D J S, CROCOS P J, GERARD P D, et al. Response to selection and heritability for growth in the Kuruma prawn Penaeus japonicus[J]. Aquaculture, 2000, 181(1-2): 215-223.
[19] GOYARD E, PATROIS J, PEIGNON J M, et al. Selection for better growth of Penaeus stylirostris in Tahiti and New Caledonia[J]. Aquaculture, 2002, 204(3-4): 461-468.
[20] FINLEY L M, HALEY L. The genetic of aggression in the juvenile American lobers, Homarus amerucanus[J]. Aquaculture, 1983, 33(1-4): 135-139.
[21] LESTER L J. Differences in larval growth among families of Penaeus stylirostris Stimpson and P.vannamei Boone[J]. Aquac Res, 1988, 19(3): 243-251.
[22] 孙苗苗, 黄建华, 杨其彬, 等. 13个斑节对虾家系的生长及抗氨氮特性比较[J]. 上海海洋大学学报, 2011, 20(4): 510-516.
[23] 周鲜娇, 邱德全, 刘文珍. 温度变化对凡纳滨对虾心率和肝胰腺抗氧化酶活性的影响[J]. 渔业现代化, 2007, 34(5): 5-7.
[24] 孙金辉, 徐赞霞, 季延滨, 等. 温度骤降对南美白对虾仔虾抗氧化机能的影响[J]. 天津农学院学报, 2008, 3(15): 8-10.
[25] 赵丙辰, 倪天崎, 李伟. 南美白对虾血细胞酚氧化酶活性的研究[J]. 青岛理工大学学报, 2006, 3(27): 65-67.
[26] 刘昌彬, 王金星, 刘存仁, 等. 非生物环境因子对暴发性流行病病原感染的中国对虾发病的影响[J]. 水产学报, 2001, 25(1): 58-63.
[27] 哈承旭, 刘萍, 何玉英, 等. 氯化铵对“黄海1号”中国对虾免疫相关酶类的影响[J]. 渔业科学进展, 2009, 1(30): 35-40.
[28] 王明, 胡义波, 姜乃澄. 氨态氮、亚硝态氮对罗氏沼虾免疫相关酶类的影响[J]. 浙江大学学报: 理学版, 2005, 6(32): 699-705.
[29] 林林, 丁美丽, 孙舰军, 等.有机污染提高对虾对病原菌易感性研究[J]. 海洋学报, 1998, 20(1): 90-93.
[30] 姜令绪, 潘鲁青, 肖国强. 氨氮对凡纳对虾免疫指标的影响[J]. 中国水产科学, 2004, 6(11): 537-539.
[31] ARGUE B J. ARCE S M, LOTZ J M, et al. Selective breeding of pacific white shrimp (Litopenaeus vannamei) for growth and resistance to Taura Syndopme Vius[J]. Aquaculture, 2002, 204(3-4): 447-460.
文章导航

/