不同环境条件下斑节对虾家系生长和免疫酶活性差异

doi:10.11978/j.issn.1009-5470.2013.03.012

热带海洋学报 ›› 2013, Vol. 32 ›› Issue (3): 78-85.doi: 10.11978/j.issn.1009-5470.2013.03.012cstr: 32234.14.j.issn.1009-5470.2013.03.012

不同环境条件下斑节对虾家系生长和免疫酶活性差异

杨其彬¹, 王专伟^{1, 2}, 周发林¹, 温为庚¹, 苏天凤¹, 邱丽华¹, 黄建华¹

1. 中国水产科学研究院南海水产研究所, 农业部南海渔业资源开发利用重点试验室, 广东广州 510300; 2. 上海海洋大学, 上海 201306

收稿日期:2011-12-22 修回日期:2012-02-29 出版日期:2013-08-27 发布日期:2013-08-28
通讯作者: 国家高技术研究发展（“863”）计划项目(2012AA10A409-02)
作者简介:杨其彬(1972—), 男, 广东省高州市人, 助理研究员, 主要从事水产动物繁育与遗传育种研究。E-mail: yangqibin1208@163.com

Analysis on growth and characteristic of immune enzymes activity of Penaeus monodon family under environment-stress

YANG Qi-bin¹, WANG Zhuan-wei^{1, 2}, ZHOU Fa-lin¹, WEN Wei-geng¹, SU Tian-feng¹, QIU Li-hua¹, HUANG Jian-hua¹

1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South China Sea Fishery Resources Utilization, Ministry of Agriculture, Guangzhou 510300, China; 2. Shanghai Ocean University, Shanghai 201306, China

Received:2011-12-22 Revised:2012-02-29 Online:2013-08-27 Published:2013-08-28
Contact: 黄建华。E-mail:hjh210440@sina.com.cn

摘要/Abstract

摘要：

研究了正常生长、低温胁迫和高氨氮胁迫下10个斑节对虾家系生长特性及超氧化物歧化酶(Superoxide Dismutase, SOD)、酸性磷酸酶(Acid Phosphatase, ACP)、酚氧化酶(Phenoloxidase, PO)3种免疫酶活性的变化。生长试验结果表明, 10个家系特定生长率(SGR)呈现明显差异, 家系077与家系017和661的ACP活性存在极显著差异, 家系4612与家系017的SOD活性存在极显著性差异, 而其他家系的免疫酶活性无显著差异。斑节对虾在48h低温胁迫下, ACP和SOD活性先升高后降低, PO活性则不断降低, 而对照组的3种酶活性呈无规律轻微变化。斑节对虾在48h高氨氮胁迫下, ACP活性不断升高, SOD在0—8h活性上升, 8—48h活性快速降低, PO活性不断下降, 而对照组的3种酶活性呈无规律轻微变化；一些家系间的3种免疫酶活性存在显著性差异, 其中家系4612的3种酶活性均高于其他家系。3个试验中, 家系的特定生长率与免疫酶活性均呈负相关。

关键词: 斑节对虾, 家系, 氨氮, 低温, 免疫酶

Abstract:

The response of Superoxide Dismutase (SOD), Acid Phosphatase (ACP), and Phenoloxidase (PO) in Penaeus monodonfamilies to normal growth, low temperature stress and high ammonia stress was studied under laboratory condition. Growth in terms of specific growth rate (SGR) was significant difference among the selected families (p<0.05). The activities of ACP were significant difference among family 077, 017 and 661. The activities of SOD were significant difference between family 4612 and family 017. When the rearing temperature decreased from 27 ℃ to 20 ℃, the activities of ACP and SOD first increased and then decreased, the activities of PO reduced prolonged according to the low temperature stress, and the minor changes of control trial were not regular. Under the high ammonia stress, the activities of ACP increased prolonged, the activities of SOD increased in 0-8 h, then rapidly decreased in 8-48 h, the activities of PO reduced in 0-48 h, and the minor changes of control trial were not regular. The activities of immune enzymes of some families were significant differences, and three immune enzymes activities of family 4612 higher than that of other families under the high ammonia stress. A negative correlation was found between the specific growth rate and the activities of ACP, SOD and PO of ten families in three trials.

Key words: Penaeus monodon, family, low temperature, ammonia, immunity enzymes activity

中图分类号:

S945.4+7

杨其彬, 王专伟, 周发林, 温为庚, 苏天凤, 邱丽华, 黄建华. 不同环境条件下斑节对虾家系生长和免疫酶活性差异[J]. 热带海洋学报, 2013, 32(3): 78-85.

YANG Qi-bin, WANG Zhuan-wei, ZHOU Fa-lin, WEN Wei-geng, SU Tian-feng, QIU Li-hua, HUANG Jian-hua. Analysis on growth and characteristic of immune enzymes activity of Penaeus monodon family under environment-stress[J]. Journal of Tropical Oceanography, 2013, 32(3): 78-85.

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不同环境条件下斑节对虾家系生长和免疫酶活性差异

Analysis on growth and characteristic of immune enzymes activity of Penaeus monodon family under environment-stress

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