从福建云霄红树林区分离筛选出一株对赤潮异弯藻(Heterosigma akashiwo)具有较强抑藻作用的细菌, 命名为HSY-03。结合16S rDNA序列分析, 该菌与芽孢杆菌属(Bacillus sp.)同源性高达99.0%。通过抑藻方式研究发现菌株是通过分泌胞外活性物质抑藻, 属于间接杀藻; 胞外活性物质为非多糖、非脂肪、非蛋白类化合物, 具有较好的光、热和酸碱稳定性; 对供试13株赤潮藻未表现出溶藻效果, 表明该菌株抑藻具有一定的种属特异性。菌株HSY-03抑藻效果具有浓度效应, 初始终浓度高于5.0%抑藻效果较好。不同生长时期菌株HSY-03对赤潮异弯藻均表现出较好的抑藻效果, 但菌株处于稳定期和衰亡期时抑藻效果最好。
傅丽君
,
林天城
,
余晓琪
,
郑天凌
. 抑藻细菌HSY-03的分离、鉴定及对赤潮异弯藻生长的影响[J]. 热带海洋学报, 2016
, 35(3)
: 87
-93
.
DOI: 10.11978/2015099
The bacterial strain HSY-03 is closely related to the genus Bacillus sp. and capable of inhibiting the toxic dinoflagellate Heterosigma akashiwo. It was isolated from the mangrove area in Yunxiao, Fujian Province, China. Based on 16S rRNA gene sequences and morphological characteristics, the strain HSY-03 was determined to be Bacillus sp. on the basis of 99.0% similarity with reference strain sequences from the NCBI. HSY-03 indirectly attacked Heterosigma akashiwo by secreting extracellular substances. The algicidal compounds could not be proteinaceous, nucleate and polysaccharide, which showed heat tolerant and being stable in UV as well as acidic and alkaline conditions. The bacterial strain was species-specific among the 13 algae tested. There was a close interaction between initial bacterial and algae cell densities. Bacterial HSY-03 showed strong algicidal activity when the initial concentration was above 5.0%. HSY-03 showed certain algicidal effects on Heterosigma akashiwo during different growth periods, especially during its stable and decline phases. These findings provided a novel viewpoint on biological control of harmful algal blooms and the relationship between the bacteria and algae.
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