抑藻细菌HSY-03的分离、鉴定及对赤潮异弯藻生长的影响

doi:10.11978/2015099

热带海洋学报 ›› 2016, Vol. 35 ›› Issue (3): 87-93.doi: 10.11978/2015099CSTR: 32234.14.2015099

抑藻细菌HSY-03的分离、鉴定及对赤潮异弯藻生长的影响

傅丽君¹, 林天城¹, 余晓琪², 郑天凌²

1. 莆田学院环境与生物工程学院, 福建省新型污染物生态毒理效应与控制重点实验室, 福建莆田 351100;
2. 厦门大学生命科学学院, 滨海湿地生态系统教育部重点实验室, 福建厦门 361005

收稿日期:2015-07-28 出版日期:2016-05-29 发布日期:2016-05-27
作者简介:作者简介：傅丽君(1975—), 女, 福建省莆田市人, 博士, 教授, 主要从事环境生态学研究。E-mail: lijun_fu@sina.com
基金资助:
国家自然科学基金(31400318); 福建省高校新世纪优秀人才支持计划; 福建省科技厅重点项目(2013N0030); 莆田市科技项目(2012S04)

Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo

FU Lijun¹, LIN Tiancheng¹, YU Xiaoqi², ZHENG Tianling²

. College of Environment and Life Science, Fujian Provincial Key Laboratory of Ecology-toxicological Effects & Control for Emerging Contaminants, Putian University, Putian 351100, China;
2. Key Laboratory of Ministry of Education for Coast and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen 361005, China

Received:2015-07-28 Online:2016-05-29 Published:2016-05-27
Contact: FU Lijun. E-mail: lijun_fu@sina.com
Supported by:
National Nature Science Foundation (31400318); New Century Excellent Talents in Fujian Province University; Key Projects of Science and Technology Department of Fujian Province (2013N0030); Science and Technology Project of Putian (2012S04)

摘要/Abstract

摘要： 从福建云霄红树林区分离筛选出一株对赤潮异弯藻(Heterosigma akashiwo)具有较强抑藻作用的细菌, 命名为HSY-03。结合16S rDNA序列分析, 该菌与芽孢杆菌属(Bacillus sp.)同源性高达99.0%。通过抑藻方式研究发现菌株是通过分泌胞外活性物质抑藻, 属于间接杀藻; 胞外活性物质为非多糖、非脂肪、非蛋白类化合物, 具有较好的光、热和酸碱稳定性; 对供试13株赤潮藻未表现出溶藻效果, 表明该菌株抑藻具有一定的种属特异性。菌株HSY-03抑藻效果具有浓度效应, 初始终浓度高于5.0%抑藻效果较好。不同生长时期菌株HSY-03对赤潮异弯藻均表现出较好的抑藻效果, 但菌株处于稳定期和衰亡期时抑藻效果最好。

关键词: 抑藻细菌, 赤潮, 异弯藻, 胞外活性物质

Abstract: The bacterial strain HSY-03 is closely related to the genus Bacillus sp. and capable of inhibiting the toxic dinoflagellate Heterosigma akashiwo. It was isolated from the mangrove area in Yunxiao, Fujian Province, China. Based on 16S rRNA gene sequences and morphological characteristics, the strain HSY-03 was determined to be Bacillus sp. on the basis of 99.0% similarity with reference strain sequences from the NCBI. HSY-03 indirectly attacked Heterosigma akashiwo by secreting extracellular substances. The algicidal compounds could not be proteinaceous, nucleate and polysaccharide, which showed heat tolerant and being stable in UV as well as acidic and alkaline conditions. The bacterial strain was species-specific among the 13 algae tested. There was a close interaction between initial bacterial and algae cell densities. Bacterial HSY-03 showed strong algicidal activity when the initial concentration was above 5.0%. HSY-03 showed certain algicidal effects on Heterosigma akashiwo during different growth periods, especially during its stable and decline phases. These findings provided a novel viewpoint on biological control of harmful algal blooms and the relationship between the bacteria and algae.

Key words: algicidal bacteria, harmful algal blooms, Heterosigma akashiwo, extracellular substances

中图分类号:

P735.53

傅丽君, 林天城, 余晓琪, 郑天凌. 抑藻细菌HSY-03的分离、鉴定及对赤潮异弯藻生长的影响[J]. 热带海洋学报, 2016, 35(3): 87-93.

FU Lijun, LIN Tiancheng, YU Xiaoqi, ZHENG Tianling. Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo[J]. Journal of Tropical Oceanography, 2016, 35(3): 87-93.

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抑藻细菌HSY-03的分离、鉴定及对赤潮异弯藻生长的影响

Isolation and identification of algicidal bacteria HSY-03 and its impact on Heterosigma akashiwo

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