热带海洋学报 ›› 2020, Vol. 39 ›› Issue (3): 76-85.doi: 10.11978/2020003

• 海洋生物学 • 上一篇    下一篇

微量元素锰对底栖甲藻热带库里亚藻(Coolia tropicalis)生长及叶绿素荧光特性的影响

刘莎莎1,2, 陈亨1,2, 黄凯旋1,2(), 吕颂辉1,2, 张亮3, 谢学东3   

  1. 1. 暨南大学赤潮与海洋生物学研究中心, 广东 广州 510632
    2. 水体富营养化与藻华防治广东省普通高校重点实验室, 广东 广州 510632
    3. 广东省环境监测中心, 广东 广州 510632
  • 收稿日期:2020-01-08 修回日期:2020-01-29 出版日期:2020-05-10 发布日期:2020-05-19
  • 通讯作者: 黄凯旋
  • 作者简介:刘莎莎(1995—), 女, 安徽省亳州市人, 硕士研究生, 研究方向为藻类光合生理, E-mail:shasha_icon@163.com
  • 基金资助:
    国家自然科学基金(41706126);国家自然科学基金(41876173);科技基础资源调查专项(2018FY100200);科技基础资源调查专项(2018FY100100)

Effect of manganese on the growth and chlorophyll fluorescence characteristics of benthic dinoflagellate Coolia tropicalis

Shasha LIU1,2, Heng CHEN1,2, Kaixuan HUANG1,2(), Songhui LÜ1,2, Liang ZHANG3, Xuedong XIE3   

  1. 1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510362, China
    2. Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, Guangzhou 510632, China
    3. Guangdong Environmental Monitoring Center, Guangzhou 510362, China
  • Received:2020-01-08 Revised:2020-01-29 Online:2020-05-10 Published:2020-05-19
  • Contact: Kaixuan HUANG
  • Supported by:
    Foundation item: National Natural Science Fund ation of China(41706126);National Natural Science Fund ation of China(41876173);Science & Technology Basic Resources Investigation Program of China(2018FY100200);Science & Technology Basic Resources Investigation Program of China(2018FY100100)

摘要:

以热带库里亚藻(Coolia tropicalis)为研究对象, 在不同锰浓度(0、1、5、10、50μmol·L -1)的人工海水培养15d, 利用叶绿素荧光动力学技术研究了其生长和光合作用对不同锰环境的响应。结果表明: 1)比生长速率(μ)和最大相对电子传递速率(rETRmax)与锰浓度均呈指数关系且对锰胁迫具有相同程度的响应; 2)锰浓度至少大于1μmol·L -1才能维持热带库里亚藻正常的光合作用活性, 当锰浓度低于该浓度时, 光合作用活性(Fv/Fm)在6d后开始下降, 而单位反应中心吸收光能(ABS/RC)和热能耗散(DI0/RC)升高; 两个反应中心之间的电子传递(φE0)及生长并未受影响, 表明此阶段锰缺乏只影响活性光反应中心数量并提高热耗散途径; 当锰缺乏延长至15d时, 胁迫作用显现(F0上升)并且电子传递(φE0)和生长受到抑制, 这阶段锰缺乏使光反应中心关闭且电子传递受阻; 3)锰缺乏的修复损伤比(r/k)并未降低, 表明锰缺乏并未影响热带库里亚藻的光保护能力。

关键词: 锰, 热带库里亚藻, 底栖甲藻, 叶绿素荧光, OJIP曲线

Abstract:

In this study, the effects of growth and chlorophyll fluorescence characteristics in Coolia tropicalis under different concentrations of manganese (Mn, 0, 1, 5, 10, and 50μmol·L -1) were investigated by using chlorophyll fluorescence kinetics technique. The results are as follows. The relationships of specific growth rate (μ) and maximal relative electron transport rate (rETRmax) with the concentration of Mn were fitted by exponential functions; growth and electron transport had the same degree of response to manganese stress. The regular activity of photosynthesis can be maintained only when the concentration of manganese is at least 1μmol·L -1. when the Mn concentration was lower than that 1μmol·L -1, the photochemical efficiency (Fv/Fm) begins to decrease after 6 days, while the per reaction center of absorbed light energy (ABS/RC) and heat energy dissipation (DI0/RC) increase, and no influence to electron transport (φE0) and the growth, which indicated Mn deficiency only decreased the number of active reaction center and increased the heat dissipation in this phase. While Mn deficiency extended to day 15, the rising of F0 and the decreases of φE0 and growth indicated that Mn deficiency caused irreversible damage to the active reaction center and inhibited the electron transport in this phase. The repair rate to damage rate (r/k) under intense light was not affected by Mn deficiency, indicated Mn deficiency did not influence the photoprotection of C. tropicalis.

Key words: manganese, Coolia tropicalis, benthic dinoflagellate, chlorophyll fluorescence, OJIP curve