中国西沙群岛底栖甲藻热带库里亚藻(Coolia tropicalis)的形态学、系统发育及毒性研究

doi:10.11978/2019129

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (3): 86-97.doi: 10.11978/2019129CSTR: 32234.14.2019129

中国西沙群岛底栖甲藻热带库里亚藻(Coolia tropicalis)的形态学、系统发育及毒性研究

黄丽芬¹, 李群¹, 吕颂辉¹(), 张亮², 谢学东²

^1. 暨南大学赤潮与海洋生物学研究中心, 广东广州 510632
^2. 广东省环境监测中心, 广东广州 510632

收稿日期:2019-12-11 修回日期:2020-02-03 出版日期:2020-05-10 发布日期:2020-05-19
通讯作者: 吕颂辉
作者简介:黄丽芬(1995—), 女, 广东省清远市人, 硕士研究生, 研究方向为藻类分类。E-mail:hhhliii22@163.com
基金资助:
国家自然科学基金项目(41876173);国家自然科学基金项目(41706126);科技基础资源调查专项(2018FY100200);科技基础资源调查专项(2018FY100200、2018FY100100)(2018FY100100)

Morphology, phylogeny and toxicity of Coolia tropicalis (Dinophyceae) from the Xisha Islands, China

Lifen HUANG¹, Qun LI¹, Songhui LÜ¹(), Liang ZHANG², Xuedong XIE²

^1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510632, China
^2. Guangdong environmental monitoring center, Guangzhou 510362, China

Received:2019-12-11 Revised:2020-02-03 Online:2020-05-10 Published:2020-05-19
Contact: Songhui Lü
Supported by:
Foundation item: National Natural Science Foundation of China(41876173);Foundation item: National Natural Science Foundation of China(41706126);Science & Technology Basic Resources Investigation Program of China(2018FY100200);Science & Technology Basic Resources Investigation Program of China(2018FY100100)

摘要/Abstract

摘要：

库里亚藻(Coolia)是一类广泛分布且具有生物毒性的海洋底栖甲藻。本研究对近年来在中国南海西沙群岛海域采集分离的4株库里亚藻, 通过光镜及电镜下的形态学观察, 并结合基于核糖体大亚基(large subunit, LSU)rDNA(D1-D3)和内部转录间隔区(internal transcribed spacer, ITS)的序列进行系统发育分析, 鉴定该4个株系为热带库里亚藻(Coolia tropicalis)。在卤虫(Artemia salina)生物毒性试验中, 热带库里亚藻不同株系毒素提取液对卤虫幼虫表现出了毒性差异, XS554株系的48h半致死浓度(LC50)为1.42mg·mL ^-1(约相当于1.1×10 ⁵cells·mL ^-1), 5XS15株系的LC50为1.92mg·mL ^-1(约相当于1×10 ⁵cells·mL ^-1)。

关键词: 热带库里亚藻, 形态学, 系统发育, 毒性, 卤虫

Abstract:

Coolia is a widespread and toxic genus of benthic marine dinoflagellate. In this study, four strains of Coolia species were examined as Coolia tropicalis, which were collected in the Xisha Islands of the South China Sea in recent years. Morphology was determined using light microscopy and scanning electron microscopy. Phylogenetic analyses were constructed based on the sequences of the large subunit rDNA and the internal transcribed spacer region. The bioassay toxicity test of brine shrimp (Artemia salina) demonstrated that the algal lysates extracted from different strains of C. tropicalis exhibited different toxic effects. At 48 h, the LC50 of XS554 strain was 1.42 mg·mL ^-1 (approximately 1.1×10 ⁵ cells·mL ^-1) and 5XS15 strain was 1.92 mg·mL ^-1 (approximately 1×10 ⁵ cells·mL ^-1).

Key words: Coolia tropicalis, morphology, phylogeny, toxicity, Artemia salina

黄丽芬, 李群, 吕颂辉, 张亮, 谢学东. 中国西沙群岛底栖甲藻热带库里亚藻(Coolia tropicalis)的形态学、系统发育及毒性研究[J]. 热带海洋学报, 2020, 39(3): 86-97.

Lifen HUANG, Qun LI, Songhui LÜ, Liang ZHANG, Xuedong XIE. Morphology, phylogeny and toxicity of Coolia tropicalis (Dinophyceae) from the Xisha Islands, China[J]. Journal of Tropical Oceanography, 2020, 39(3): 86-97.

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株系号	采样时间	采样点	经纬度	附着基质
2XS73	2016-06-11	银屿岛(S1)	16°34'15.39"N, 111°42'13.71"E	死珊瑚, 少量活珊瑚
3XS45	2016-06-12	鸭公岛(S2)	16°33'30.29"N, 111°40'40.56"E	死珊瑚
XS554	2015-06-11	永兴岛(S3)	16°33'30.29"N, 111°40'40.56"E	礁岩底质
5XS15	2015-06-11	赵述岛(S4)	16°57'18.73"N, 112°15'58.50"E	礁石底, 死珊瑚

种名	Coolia tropicalis	Coolia palmyrensis	Coolia malayensis	Coolia monotis	Coolia santacroce	Coolia guanchica
C. tropicalis
C. palmyrensis	0.421
C. malayensis	0.434	0.146
C. monotis	0.450	0.170	0.117
C. santacroce	0.428	0.098	0.090	0.086
C. guanchica	0.365	0.394	0.431	0.425	0.361
C. canariensis	0.392	0.354	0.396	0.416	0.359	0.187

种名	Coolia tropicalis	Coolia canariensis	Coolia palmyrensis	Coolia santacroce	Coolia monotis
C. tropicalis
C. canariensis	0.552
C. palmyrensis	0.553	0.798
C. santacroce	0.477	0.737	0.397
C. monotis	0.428	0.577	0.379	0.201
C. malayensis	0.539	0.651	0.379	0.209	0.128

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中国西沙群岛底栖甲藻热带库里亚藻(Coolia tropicalis)的形态学、系统发育及毒性研究

Morphology, phylogeny and toxicity of Coolia tropicalis (Dinophyceae) from the Xisha Islands, China

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