微量元素锰对底栖甲藻热带库里亚藻(Coolia tropicalis)生长及叶绿素荧光特性的影响

doi:10.11978/2020003

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (3): 76-85.doi: 10.11978/2020003CSTR: 32234.14.2020003

微量元素锰对底栖甲藻热带库里亚藻(Coolia tropicalis)生长及叶绿素荧光特性的影响

刘莎莎^1,², 陈亨^1,², 黄凯旋^1,²(), 吕颂辉^1,², 张亮³, 谢学东³

^1. 暨南大学赤潮与海洋生物学研究中心, 广东广州 510632
^2. 水体富营养化与藻华防治广东省普通高校重点实验室, 广东广州 510632
^3. 广东省环境监测中心, 广东广州 510632

收稿日期:2020-01-08 修回日期:2020-01-29 出版日期:2020-05-10 发布日期:2020-05-19
通讯作者: 黄凯旋
作者简介:刘莎莎(1995—), 女, 安徽省亳州市人, 硕士研究生, 研究方向为藻类光合生理, E-mail:shasha_icon@163.com
基金资助:
国家自然科学基金(41706126);国家自然科学基金(41876173);科技基础资源调查专项(2018FY100200);科技基础资源调查专项(2018FY100100)

Effect of manganese on the growth and chlorophyll fluorescence characteristics of benthic dinoflagellate Coolia tropicalis

Shasha LIU^1,², Heng CHEN^1,², Kaixuan HUANG^1,²(), Songhui LÜ^1,², Liang ZHANG³, Xuedong XIE³

^1. Research Center for Harmful Algae and Marine Biology, Jinan University, Guangzhou 510362, China
^2. Key Laboratory of Aquatic Eutrophication and Control of Harmful Algae Blooms of Guangdong Higher Education Institutes, Guangzhou 510632, China
^3. Guangdong Environmental Monitoring Center, Guangzhou 510362, China

Received:2020-01-08 Revised:2020-01-29 Online:2020-05-10 Published:2020-05-19
Contact: Kaixuan HUANG
Supported by:
Foundation item: National Natural Science Fund ation of China(41706126);National Natural Science Fund ation of China(41876173);Science & Technology Basic Resources Investigation Program of China(2018FY100200);Science & Technology Basic Resources Investigation Program of China(2018FY100100)

摘要/Abstract

摘要：

以热带库里亚藻(Coolia tropicalis)为研究对象, 在不同锰浓度(0、1、5、10、50μmol·L ^-1)的人工海水培养15d, 利用叶绿素荧光动力学技术研究了其生长和光合作用对不同锰环境的响应。结果表明: 1)比生长速率(μ)和最大相对电子传递速率(rETR_max)与锰浓度均呈指数关系且对锰胁迫具有相同程度的响应; 2)锰浓度至少大于1μmol·L ^-1才能维持热带库里亚藻正常的光合作用活性, 当锰浓度低于该浓度时, 光合作用活性(F_v/F_m)在6d后开始下降, 而单位反应中心吸收光能(ABS/RC)和热能耗散(DI₀/RC)升高; 两个反应中心之间的电子传递(φ_E0)及生长并未受影响, 表明此阶段锰缺乏只影响活性光反应中心数量并提高热耗散途径; 当锰缺乏延长至15d时, 胁迫作用显现(F₀上升)并且电子传递(φ_E0)和生长受到抑制, 这阶段锰缺乏使光反应中心关闭且电子传递受阻; 3)锰缺乏的修复损伤比(r/k)并未降低, 表明锰缺乏并未影响热带库里亚藻的光保护能力。

关键词: 锰, 热带库里亚藻, 底栖甲藻, 叶绿素荧光, OJIP曲线

Abstract:

In this study, the effects of growth and chlorophyll fluorescence characteristics in Coolia tropicalis under different concentrations of manganese (Mn, 0, 1, 5, 10, and 50μmol·L ^-1) were investigated by using chlorophyll fluorescence kinetics technique. The results are as follows. The relationships of specific growth rate (μ) and maximal relative electron transport rate (rETR_max) with the concentration of Mn were fitted by exponential functions; growth and electron transport had the same degree of response to manganese stress. The regular activity of photosynthesis can be maintained only when the concentration of manganese is at least 1μmol·L ^-1. when the Mn concentration was lower than that 1μmol·L ^-1, the photochemical efficiency (F_v/F_m) begins to decrease after 6 days, while the per reaction center of absorbed light energy (ABS/RC) and heat energy dissipation (DI₀/RC) increase, and no influence to electron transport (φ_E0) and the growth, which indicated Mn deficiency only decreased the number of active reaction center and increased the heat dissipation in this phase. While Mn deficiency extended to day 15, the rising of F₀ and the decreases of φ_E0 and growth indicated that Mn deficiency caused irreversible damage to the active reaction center and inhibited the electron transport in this phase. The repair rate to damage rate (r/k) under intense light was not affected by Mn deficiency, indicated Mn deficiency did not influence the photoprotection of C. tropicalis.

Key words: manganese, Coolia tropicalis, benthic dinoflagellate, chlorophyll fluorescence, OJIP curve

刘莎莎, 陈亨, 黄凯旋, 吕颂辉, 张亮, 谢学东. 微量元素锰对底栖甲藻热带库里亚藻(Coolia tropicalis)生长及叶绿素荧光特性的影响[J]. 热带海洋学报, 2020, 39(3): 76-85.

Shasha LIU, Heng CHEN, Kaixuan HUANG, Songhui LÜ, Liang ZHANG, Xuedong XIE. Effect of manganese on the growth and chlorophyll fluorescence characteristics of benthic dinoflagellate Coolia tropicalis[J]. Journal of Tropical Oceanography, 2020, 39(3): 76-85.

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参数及其计算	定义
F₀	暗适应后的最小荧光强度
F_m=F_P	暗适应后的最大荧光值
V_J = (F_J - F₀) / (F_m - F₀)	在J点的相对可变荧光
V_I= (F_I- F₀) / (F_m - F₀)	在I点的相对可变荧光
F_v / F₀ = (F_m- F₀) -1	PSⅡ的潜在活性
F_v / F_m	PSⅡ最大光化学效率
M₀ = 4 (F_300μs - F₀) / (F_m - F₀)	OJIP荧光诱导曲线的初始斜率
Area	荧光诱导曲线与F=Fm之间的面积
S_m = Area / (F_m - F₀)	标准化后的在OJIP荧光诱导曲线和F=F_m之间的面积
ABS / RC = M₀ (1/V_J) · (1/φ_P0)	单位反应中心吸收的光能
TR₀ / RC = M₀ (1/V_J)	单位反应中心捕获的用于还原Q_A的能量(在t=0时)
ET₀ / RC = M₀ (1/V_J)ψ₀	单位反应中心捕获的用于电子传递的能量(在t=0时)
DI₀ / RC = (ABS / RC) - (TR₀ / RC)	单位反应中心的热耗散(在t=0时)
φ_P0= TR₀/ ABS =(1- F₀/F_m)	最大光化学效率(在 t=0 时)
φ₀ = ET₀/ TR₀=(1- V_J)	捕获的激子将电子传递到电子传递链中超过 Q_A的其它电子受体的概率(在t=0时)
φ_E0= ET₀/ ABS =(1- F₀/ F_m) φ₀	用于电子传递的量子产额(在t=0时)
φ_D0= 1- φ_P0= (F₀/ F_m)	用于热耗散的量子比率(在t=0时)
PI_ABS= RC/ABS×[φ_P0/(1- φ_P0)] [φ₀/ (1- φ₀)]	以吸收光能为基础的性能指数

参数	初始斜率	饱和锰浓度/(μmol·L^-1)
μ/(d^-1)	0.24 ± 0.03	48.66 ± 5.49
rETR_max/(μmol e^-·m^-2·s^-1)	0.25 ± 0.02	17.29 ± 1.12
E_k/(μmol photons·m^-2·s^-1)	0.09 ± 0.04	49.74 ± 19.67
α	0.31 ± 0.03	15.72 ± 1.47

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微量元素锰对底栖甲藻热带库里亚藻(Coolia tropicalis)生长及叶绿素荧光特性的影响

Effect of manganese on the growth and chlorophyll fluorescence characteristics of benthic dinoflagellate Coolia tropicalis

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