热带海洋学报 ›› 2010, Vol. 29 ›› Issue (6): 118-124.doi: 10.11978/j.issn.1009-5470.2010.06.118

• 海洋生物学 • 上一篇    下一篇

斜带石斑鱼烂尾病病原菌的分离与鉴定

梅冰, 周永灿, 徐先栋, 王世峰, 谢珍玉   

  1. 海南大学海洋学院, 海南 海口 570228
  • 收稿日期:2009-02-10 修回日期:2009-11-15 出版日期:2010-12-15 发布日期:2010-12-15
  • 通讯作者: 谢珍玉。
  • 作者简介:梅冰(1982—), 男, 湖北省武汉市人, 硕士研究生, 研究方向为水生生物病害及其控制。E-mail: meibing11meibing@163.com
  • 基金资助:

    农业科技成果转化项目(2009GB2E200302); 国家自然科学基金项目(30660144、30760190、31060360); 海南省自然科学基金
    项目(80617); 海南省教育厅高等学校科研项目(Hj200729)

Isolation and identification of bacteria pathogens from Epinephelus coioides with tail-rotted disease

MEI Bing, ZHOU Yong-can, XU Xian-dong, WANG Shi-feng, XIE Zhen-yu   

  1. College of Marine Science, Hainan University, Haikou 570228, China
  • Received:2009-02-10 Revised:2009-11-15 Online:2010-12-15 Published:2010-12-15
  • Contact: 谢珍玉。
  • About author:梅冰(1982—), 男, 湖北省武汉市人, 硕士研究生, 研究方向为水生生物病害及其控制。E-mail: meibing11meibing@163.com
  • Supported by:

    农业科技成果转化项目(2009GB2E200302); 国家自然科学基金项目(30660144、30760190、31060360); 海南省自然科学基金
    项目(80617); 海南省教育厅高等学校科研项目(Hj200729)

摘要:

2008年5月从海南省三亚红沙港与陵水新村湾网箱养殖的患“烂尾病”的斜带石斑鱼Epinephelus coioides中分离到3株优势菌株HS08001、HS08002和XC08001。人工感染试验表明, 这3株菌株为斜带石斑鱼的致病菌。经菌株形态特征、培养特性和生理生化反应等表型测定以及16S rDNA测序分析, 这3株细菌被鉴定为哈维氏弧菌Vibrio harveyi。其中, HS08001对斜带石斑鱼的半致死剂量为1.05×104CFU•g?1 鱼体重。药敏试验表明, 该菌具有较强的耐药性, 在所检测的25中抗菌药物中, 仅对庆大霉素、氯霉素和利福平高度敏感, 而对阿莫西林等18种抗菌药物不敏感。

关键词: 斜带石斑鱼Epinephelus coioides, 哈维氏弧菌Vibrio harveyi, 烂尾病, 16S rDNA

Abstract:

A very serious tail-rotted disease broken out in the cage-cultured Epinephelus coioides in Sanya city and Lingshui County, Hainan province, China in May 2008. Three strains of HS08001, HS08002, and XC08001 were isolated from the dis-eased grouper and were shown to be the pathogens by artificial infection. All the three isolates were identified as Vibrio har-veyi by biochemical tests and 16S rDNA sequence analysis. The LD50 value of strain HS08001 for E. coioides was 1.05×104 CFU/g body weight. Sensitivity test to 25 chemotherapeutic agents indicated that the pathogen was only highly sensitive to gentamicin, chloramphenicol, and rifampicin.

Key words: Epinephelus coioides, Vibrio harveyi, tail-rotted disease, 16s rDNA