柳珊瑚Leptogorgia rigida中甾体类成分研究

doi:10.11978/2018125

热带海洋学报 ›› 2019, Vol. 38 ›› Issue (4): 64-69.doi: 10.11978/2018125CSTR: 32234.14.2018125

柳珊瑚Leptogorgia rigida中甾体类成分研究

肖细姬,邓芸,谢路凤,张琪,韦霞,于鑫,张翠仙()

广州中医药大学中药学院, 广东广州510006

收稿日期:2018-11-21 修回日期:2019-01-04 出版日期:2019-07-20 发布日期:2019-07-21
作者简介:肖细姬(1983—), 女, 助理实验师, 研究方向: 药效物质基础研究。E-Mail: xiaoxiji@gzucm.edu.cn
基金资助:
国家自然科学基金项目((81741160););广东省科技计划((2015A020216017, 2017A020217008););广州中医药大学“薪火计划”重点项目((XH20170110););海南大学南海海洋资源利用国家重点实验室开放课题((2016003););广东省自然科学基金项目((2014A030313411).)

Research on Sterols from Leptogorgia rigida

Xiji XIAO,Yun DENG,Lufeng XIE,Qi ZHANG,Xia WEI,Xin YU,Cuixian ZHANG()

School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, 510006, China

Received:2018-11-21 Revised:2019-01-04 Online:2019-07-20 Published:2019-07-21
Supported by:
National Natural Science Foundation((81741160););Science and Technology Planning Project of Guangdong Province((2015A020216017, 2017A020217008););The Fire Plan Project of the Guangzhou University of Chinese Medicine((XH20170110););Open Subject of State Key Laboratory of Marine Resources Utilization in the South China Sea of Hainan University((2016003););Natural Science Foundation of Guangdong Province((2014A030313411).)

摘要/Abstract

摘要：

首次对南海柳珊瑚Leptogorgia rigida中甾体类次生代谢产物进行研究。采用硅胶柱柱层析、十八烷基硅烷键合硅胶填料、Sephadex LH-20、高效液相色谱(HPLC)及薄层色谱(TLC)等多种方法进行分离纯化, 通过核磁共振波谱(NMR)、质谱(MS)等现代波谱分析及物理常数对照等方法进行结构鉴定。从南海柳珊瑚Leptogorgia rigida次生代谢产物中分离得到8个甾类化合物, 结构依次为: 3β, 6β, 11-trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (1); 3β, 6α, 11- trihydroxy-9, 11-seco-5α-chloest-7-en-9-one (2); 3β, 6α, 11-trihydroxy-5α-chloest-7, 22(E)-dien-9-one (3); pregnan-4-en-3, 20-dione (4); 5α-pregnan-3, 20-dione (5); 3β-hydroxy-pregnan-5-en-20-dion (6); 3β-hydroxy-5α-pregnan-20-one (7); 和3β, 6α, 11-trihydroxy-9, 11-seco-5α-gorgosterol (8)。所有化合物均首次从Leptogorgia属中得到。

关键词: 南海柳珊瑚Leptogorgia rigida, 甾类化合物, 断甾醇, 结构鉴定

Abstract:

Research was carried out on novel secondary metabolites from Leptogorgia rigida collected from the South China Sea. The secondary metabolites were isolated by silica gel column chromatography, ODS, Sephadex LH-20, thin layer chromatography, and high performance liquid chromatography (HPLC). The structures of compounds were identified by nuclear magnetic resonance spectrum (NMR), mass spectrum (MS), and literature analysis. Eight sterols were obtained and elucidated as follows: 3β, 6β, 11-trihydroxy-24-methyl-5α-chloest-9, 11-seco-7-en-9-one (1); 3β, 6α, 11-trihydroxy-24- methyl-5α-chloest-9, 11-seco-7-en-9-one (2); 3β, 6α, 11-tri- hydroxy-5α-chloest-9, 11-seco-7, 22(E)- dien-9-one (3); pregnan- 4-en-3, 20-dione (4); 5α-pregnan-3, 20-dione (5); 3β-hydroxy- pregnan-5-en-20-dione (6); 3β-hydroxy-5α-pregnan-20-one (7); and Gorgosterol (8). All the sterols were first obtained from Leptogorgia rigida.

Key words: Leptogorgia rigida, secondary metabolites, secosterols, identification

肖细姬,邓芸,谢路凤,张琪,韦霞,于鑫,张翠仙. 柳珊瑚Leptogorgia rigida中甾体类成分研究[J]. 热带海洋学报, 2019, 38(4): 64-69.

Xiji XIAO,Yun DENG,Lufeng XIE,Qi ZHANG,Xia WEI,Xin YU,Cuixian ZHANG. Research on Sterols from Leptogorgia rigida[J]. Journal of Tropical Oceanography, 2019, 38(4): 64-69.

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柳珊瑚Leptogorgia rigida中甾体类成分研究

Research on Sterols from Leptogorgia rigida

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