香港牡蛎在综合养殖池塘育肥期间不同组织的碳、氮稳定同位素周转

doi:10.11978/2022175

热带海洋学报 ›› 2023, Vol. 42 ›› Issue (4): 125-132.doi: 10.11978/2022175CSTR: 32234.14.2022175

香港牡蛎在综合养殖池塘育肥期间不同组织的碳、氮稳定同位素周转

周晖¹(), 汤保贵¹(), 伍栩民¹, 彭梓峰¹, 钟培贵², 于鸽³, 孔繁森¹

1.广东海洋大学水产学院, 广东湛江 524088
2.湛江海思特水产科技有限公司, 广东湛江 524099
3.湛江恒兴养殖技术服务有限公司, 广东湛江 524094

收稿日期:2022-08-03 修回日期:2022-10-24 出版日期:2023-07-10 发布日期:2022-12-08
作者简介:
周晖(1978—), 男, 广西玉林市人, 博士, 从事水产动物生理生态学研究。email: 346005548@qq.com
基金资助:
国家海洋局“十三五”海洋经济创新发展示范市项目(XM-202012-03B2)

Stable carbon and nitrogen isotope turnover of different tissues of the Hongkong oyster at the fattening stage in a polyculture pond

ZHOU Hui¹(), TANG Baogui¹(), WU Xumin¹, PENG Zifeng¹, ZHONG Peigui², YU Ge³, KONG Fansen¹

1. Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
2. Haiste (Zhanjiang) Aquatic Technology Co., Ltd., Zhanjiang 524099, China
3. Guangdong Evergreen Breeding Technology Service Co., Ltd., Zhanjiang 524094, China

Received:2022-08-03 Revised:2022-10-24 Online:2023-07-10 Published:2022-12-08
Supported by:
State Oceanic Administration “The Thirteenth Five-Year Plan” Marine Economic Innovation and Development Demonstration City Project(XM-202012-03B2)

摘要/Abstract

摘要：

本研究采集了在鱼虾混养池塘育肥的香港牡蛎样品, 监测牡蛎体质量、软体组织质量、出肉率, 以及闭壳肌、外套膜和鳃的δ¹³C和δ¹⁵N值随育肥时间的变化, 计算了牡蛎3种软体组织的代谢率常数m和碳、氮稳定同位素周转时间, 为开展综合养殖池塘生态系统的食物网研究提供基础数据。结果显示: 经过44d的育肥, 虽然牡蛎体质量无显著增加(P>0.05), 但软体组织质量和出肉率显著上升(P<0.05)。育肥期间牡蛎外套膜、闭壳肌和鳃的生长与整个软体组织的生长基本同步。牡蛎各组织的δ¹³C和δ¹⁵N值在育肥16d后就显著减小(P<0.05)。比较同一天采集的组织, 牡蛎闭壳肌的δ¹³C和δ¹⁵N值都显著大于外套膜和鳃(P<0.05), 而外套膜和鳃的δ¹³C和δ¹⁵N值则无显著差异(P>0.05)。闭壳肌和外套膜的代谢周转常数m为0, 其碳、氮稳定同位素周转完全由生长决定, 因此闭壳肌和外套膜碳、氮稳定同位素的周转时间相同, t₅₀和t₉₅分别为40.77d和176.22d。鳃的碳、氮稳定同位素代谢周转常数m分别为1.09%·d^-1和1.76%·d^-1, 说明鳃的碳、氮稳定同位素周转还受到代谢的影响; 鳃氮稳定同位素周转的t₅₀和t₉₅分别为20.02d和86.53d, 而碳稳定同位素周转的t₅₀和t₉₅分别为24.85d和107.42d。在本研究的育肥期间, 香港牡蛎闭壳肌、外套膜和鳃的δ¹³C和δ¹⁵N值具有鲜明特点, 并且随育肥时间延长呈现出迅速且有规律的变化, 表明香港牡蛎能够反映池塘的碳、氮稳定同位素基本特征, 适合作为中、长时间尺度的基线生物用于综合养殖池塘生态系统的食物网研究。

关键词: 香港牡蛎, 育肥, 碳、氮稳定同位素, 周转

Abstract:

In order to provide basic data for the study of food webs in the polyculture pond ecosystem, the Hongkong oyster samples were collected during fattening stage in a fish-shrimp polyculture pond, and the oyster body mass, soft tissue mass, meat yield, and changes in δ¹³C and δ¹⁵N values of adductor muscle, mantle and ctenidia during the fattening stage were monitored; the metabolic rate constant m and stable carbon and nitrogen isotope turnover time of three soft tissues were calculated. The results showed that after 44 days of fattening, although the body mass of oysters did not increase significantly (P>0.05), the soft body tissue mass and meat yield increased significantly(P<0.05). The growth of mantle, adductor muscle and ctenidia were basically synchronized with the growth of whole soft tissue. The δ¹³C and δ¹⁵N values of oyster tissues decreased significantly (P<0.05) after 16 days of fattening (P<0.05). Comparing the δ¹³C and δ¹⁵N values of the three tissues collected on the same day, the values of adductor muscle were significantly larger than those of mantle and ctenidia (P<0.05), while there was no significant difference between values of mantle and ctenidia (P>0.05). The metabolic turnover constant m of adductor muscle and mantle was zero, and their stable carbon and nitrogen isotope turnover were completely determined by growth; therefore the turnover time of stable carbon and nitrogen isotope turnover in adductor muscle and mantle were the same, t₅₀ and t₉₅ were 40.77d and 176.22d, respectively. The stable carbon and nitrogen isotope metabolic turnover constant m of ctenidia were 1.09%·d^-1 and 1.76%·d^-1, respectively, indicating that the stable carbon and nitrogen isotope turnover of ctenidia were also affected by metabolism. The t₅₀ and t₉₅ of ctenidia stable nitrogen isotope turnover were 20.02d and 86.53d, respectively, while the t₅₀ and t₉₅ of stable carbon isotope turnover were 24.85d and 107.42d, respectively. During the fattening stage of this study, the δ¹³C and δ¹⁵N values of the adductor muscle, mantle and ctenidia of Hongkong oyster had distinct characteristics, and showed rapid and regular changes with the extension of the fattening time, which indicated the Hongkong oyster could reflect the basic characteristics of stable carbon and nitrogen isotopes in polyculture pond, and is suitable as a medium and long time scale baseline organism for food web research in polyculture pond ecosystem.

Key words: Crassostrea hongkongensis, fattening, stable carbon and nitrogen isotopes, turnover

周晖, 汤保贵, 伍栩民, 彭梓峰, 钟培贵, 于鸽, 孔繁森. 香港牡蛎在综合养殖池塘育肥期间不同组织的碳、氮稳定同位素周转[J]. 热带海洋学报, 2023, 42(4): 125-132.

ZHOU Hui, TANG Baogui, WU Xumin, PENG Zifeng, ZHONG Peigui, YU Ge, KONG Fansen. Stable carbon and nitrogen isotope turnover of different tissues of the Hongkong oyster at the fattening stage in a polyculture pond[J]. Journal of Tropical Oceanography, 2023, 42(4): 125-132.

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项目		初始样品	育肥8d	育肥16d	育肥44d
外套膜	δ¹⁵N/‰	9.84±0.40^c	9.70±0.82^c	8.59±0.53^b	7.38±0.22^a
外套膜	δ¹³C/‰	-21.81±1.21^c	-22.09±0.94^c	-22.38±0.81^b	-24.45±0.39^a
闭壳肌	δ¹⁵N/‰	10.93±0.23^c	10.70±0.73^c	9.71±0.76^b	8.64±0.50^a
闭壳肌	δ¹³C/‰	-20.07±0.17^c	-20.45±0.53^bc	-20.97±0.79^b	-22.16±0.63^a
鳃	δ¹⁵N/‰	9.97±0.17^c	9.50±0.69^c	8.24±0.34^b	7.34±0.51^a
鳃	δ¹³C/‰	-21.28±0.48^c	-22.08±0.57^bc	-22.64±0.33^b	-24.00±0.65^a

项目		初始样品	育肥8d	育肥16d	育肥44d
δ¹⁵N/‰	外套膜	9.84±0.40^a	9.70±0.82^a	8.59±0.53^a	7.38±0.22^a
	闭壳肌	10.93±0.23^b	10.70±0.73^b	9.71±0.76^b	8.64±0.50^b
	鳃	9.97±0.17^a	9.50±0.69^a	8.24±0.34^a	7.34±0.51^a
δ¹³C/‰	外套膜	-21.81±1.21^a	-22.09±0.94^a	-22.38±0.81^a	-24.45±0.39^a
	闭壳肌	-20.07±0.17^b	-20.45±0.53^b	-20.97±0.79^b	-22.16±0.63^b
	鳃	-21.28±0.48^a	-22.08±0.57^a	-22.64±0.33^a	-24.00±0.65^a

香港牡蛎在综合养殖池塘育肥期间不同组织的碳、氮稳定同位素周转

Stable carbon and nitrogen isotope turnover of different tissues of the Hongkong oyster at the fattening stage in a polyculture pond

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