海菊蛤血细胞类型和吞噬功能的研究

doi:10.11978/2024108

热带海洋学报 ›› 2025, Vol. 44 ›› Issue (2): 84-91.doi: 10.11978/2024108CSTR: 32234.14.2024108

海菊蛤血细胞类型和吞噬功能的研究

周可欣(), 甄雯婧, 张文文, 王江勇()

惠州学院, 广东惠州 516000

收稿日期:2024-05-25 修回日期:2024-07-22 出版日期:2025-03-10 发布日期:2025-04-11
通讯作者: 王江勇
作者简介:
周可欣(2002—), 女, 广东省汕头市人, 本科生。email: 3072997544@qq.com
基金资助:
广东省教育厅海洋贝类生态养殖与病害防控创新团队项目(2021KCXTD057); 惠州学院教授、博士启动项目(15604220001)

Morphological classification and phagocytosis of haemocytes in Spondylus sp.

ZHOU Kexin(), ZHEN Wenjing, ZHANG Wenwen, WANG Jiangyong()

Huizhou University, Huizhou 516000, China

Received:2024-05-25 Revised:2024-07-22 Online:2025-03-10 Published:2025-04-11
Contact: WANG Jiangyong
Supported by:
Guangdong Provincial Department of Education Marine Shellfish Ecological Aquaculture and Disease Prevention and Control Innovation Team Project(2021KCXTD057); Professors and doctors of Huizhou University started the project(15604220001)

摘要/Abstract

摘要：

在贝类免疫防御体系中, 血细胞作为其主要的免疫效应细胞, 起着非常重要的作用。文章通过血细胞计数、Giemsa染色、透射电镜、扫描电镜和荧光吞噬的方法研究了海菊蛤血细胞的形态与类型及吞噬功能。海菊蛤的血细胞密度为3.296 × 10⁶cells·mL^-1, 血细胞在大小和胞质内是否含有颗粒方面存在显著差异, 基于这些差异, 将其细分为透明细胞(占9.74%)、颗粒细胞(占58.14%)、无颗粒细胞(占27.50%)和淋巴样细胞(占4.62%) 4种类型。透射电镜下可以观察到透明细胞、颗粒细胞、无颗粒细胞和淋巴样细胞。扫描电镜观察, 可以明确区分出4种不同形态的细胞, 它们分别是椭圆细胞、圆形细胞、梭形细胞、核形(锥形)细胞。利用标记了绿色荧光蛋白(green fluorescent protein, GFP)的大肠杆菌BL21进行血细胞吞噬试验, 结果显示, 海菊蛤血细胞的吞噬作用主要依赖于颗粒细胞来完成。

关键词: 海菊蛤, 血细胞, 形态分类, 颗粒细胞, 无颗粒细胞, 吞噬作用

Abstract:

Blood cells, as the main immune effector cells, play a very important role in the immune defense system of shellfish. The morphology, type and phagocytosis function of hemocytes of Spondylus sp. were studied by means of blood cell count, Giemsa staining, transmission electron microscopy, scanning electron microscopy and fluorescence phagocytosis. The results showed that the blood cell density of the Spondylus sp. was 3.296 × 10⁶ cells·mL^-1, and the blood cells differ significantly in size and with regard to whether they contain particles within the cytoplasm. Based on these differences, they are subdivided them into four types: hyalinocyte (9.74%), granulocyte (58.14%), agranular haemocyte (27.50%) and lymphoid (4.62%). Hyalinocytes, agranular haemocytes, granulocytes and lymphoids could be observed under transmission electron microscope. Under the observation of scanning electron microscope, four different types of cells can be clearly distinguished, which are oval cells, round cells, spindle cells, nucleated (conical) cells. A phagocytosis experiment was carried out with Escherichia coli BL21 labeled with the green fluorescent protein (GFP), and the results showed that the phagocytosis of the blood cells from Spondylus sp. was mainly dependent on granulocytes.

Key words: Spondylus sp., haemocyte, morphological classification, granulocyte, agranulocyte, phagocytosis

中图分类号:

P735.2

周可欣, 甄雯婧, 张文文, 王江勇. 海菊蛤血细胞类型和吞噬功能的研究[J]. 热带海洋学报, 2025, 44(2): 84-91.

ZHOU Kexin, ZHEN Wenjing, ZHANG Wenwen, WANG Jiangyong. Morphological classification and phagocytosis of haemocytes in Spondylus sp.[J]. Journal of Tropical Oceanography, 2025, 44(2): 84-91.

图/表 5

图1

图2

图3

图4

图5

参考文献 15

[1]	郭磊, 2007. 池蝶蚌血细胞类型及吞噬活性的研究[D]. 南昌: 南昌大学.
	GUO LEI, 2007. Classification and phagocytosis property of haemocytes in the Hyriopsis schlegeli[D]. Nanchang: Nanchang University (in Chinese with English abstract).
[2]	裴鹏祖, 胡宝庆, 谢彦海, 等, 2011. 中华圆田螺血细胞的分类和吞噬活性[J]. 南昌大学学报(理科版), 35(1): 83-89, 94.
	PEI PENGZU, HU BAOQING, XIE YANHAI, et al, 2011. Classification and phagocytosis property of hemocytes from Viviparus chinensis[J]. Journal of Nanchang University (Natural Science Edition), 35(1): 83-89, 94 (in Chinese with English abstract).
[3]	宋小瑞, 2016. 长牡蛎免疫系统的发生和发育机制的初步研究[D]. 青岛: 中国科学院研究生院(海洋研究所).
	SONG XIAORUI, 2016. Study on the ontogenesis and development mechanism of immune system in the Pacific oyster Crassostrea gigas[D]. Qingdao: Institute of Oceanography, Chinese Academy of Sciences (in Chinese with English abstract).
[4]	孙虎山, 李光友, 2003. 栉孔扇贝血细胞的分类[J]. 海洋学报, 25(S2): 51-57.
	SUN HUSHAN, LI GUANGYOU, 2003. Classification of haemocytes of Chlamys farreri[J]. Haiyang Xuebao, 25(S2): 51-57 (in Chinese with English abstract).
[5]	王江勇, 郭志勋, 冯娟, 等, 2010. 杂色鲍血细胞免疫特点及免疫功能的研究[J]. 热带海洋学报, 29(3): 71-76.
	WANG JIANGYONG, GUO ZHIXUN, FENG JUAN, et al, 2010. Studies on immune feature and immune function of hemocytes in Haliotis diversicolor reeve[J]. Journal of Tropical Oceanography, 29(3): 71-76 (in Chinese with English abstract).
[6]	谢彦海, 2007. 褶纹冠蚌血细胞及其免疫特性研究[D]. 南昌: 南昌大学.
	XIE YANHAI, 2007. Studies on hemocytes and their immunological charaterization of Cristaria plicata leach[D]. Nanchang: Nanchang University (in Chinese with English abstract).
[7]	邢婧, 2002. 栉孔扇贝(Chlamys farreri)血细胞及其免疫特性的研究[D]. 青岛: 中国海洋大学.
	XING JING, 2002. Characterization haemocyte of seallop (Chlamys farreri) in immunology[D]. Qingdao: Ocean University of China (in Chinese with English abstract).
[8]	杨军, 石安静, 2002. 3种淡水育珠河蚌血细胞类型的研究[J]. 四川大学学报(自然科学版), 39(S1): 68-72.
	YANG JUN, SHI ANJING, 2002. Study on the classification in hemocytes of three kinds of freshwater pearl clams[J]. Journal of Sichuan University (Natural Science Edition), 39(S1): 68-72 (in Chinese with English abstract).
[9]	杨丽佳, 武梅, 王信超, 等, 2011. 厚壳贻贝血细胞及血清抗菌肽的分析[J]. 中国农学通报, 27(23): 58-64.
	YANG LIJIA, WU MEI, WANG XINCHAO, et al, 2011. Analysis of antimicrobial peptides from blood and serum of Mytilus coruscus[J]. Chinese Agricultural Science Bulletin, 27(23): 58-64 (in Chinese with English abstract).
[10]	杨新春, 2006. 扇贝血细胞的形态学研究[D]. 青岛: 中国海洋大学.
	YANG XINCHUN, 2006. Morphological studies of heamocytes of scallops, Chlamys farreri and Argopecten irradians[D]. Qingdao: Ocean University of China (in Chinese with English abstract).
[11]	朱泽闻, 徐立蒲, 吴信忠, 等, 2011. 泥蚶血细胞的形态结构特征及部分免疫功能[J]. 水产学报, 35(10): 1494-1504.
	ZHU ZEWEN, XU LIPU, WU XINZHONG, et al, 2011. Morphological, structural characteristics and phagocytic and enzymatic activities of haemocytes in blood clam Tegillarca granosa[J]. Journal of Fisheries of China, 35(10): 1494-1504 (in Chinese with English abstract).
[12]	FABRELLO J, PAGANO M, ARRIGO F, et al, 2022. Identification of haemocytes and histological examination of gills of the spiny oyster Spondylus gaederopus (Linnaeus, 1758)[J]. Fish & Shellfish Immunology, 130: 164-174.
[13]	MAO FAN, MU HUAWEI, WONG N K, et al, 2020. Hemocyte phagosomal proteome is dynamically shaped by cytoskeleton remodeling and interorganellar communication with endoplasmic reticulum during phagocytosis in a marine invertebrate, Crassostrea gigas[J]. Scientific Reports, 10(1): 6577.
[14]	WOOTTON E C, DYRYNDA E A, RATCLIFFE N A, 2003. Bivalve immunity: comparisons between the marine mussel (Mytilus edulis), the edible cockle (Cerastoderma edule) and the razor-shell (Ensis siliqua)[J]. Fish & Shellfish Immunology, 15(3): 195-210.
[15]	ZHANG XIANGYU, MAO FAN, WONG N K, et al, 2020. CLIC2α chloride channel orchestrates immunomodulation of hemocyte phagocytosis and bactericidal activity in Crassostrea gigas[J]. iScience, 23(7): 101328.

海菊蛤血细胞类型和吞噬功能的研究

Morphological classification and phagocytosis of haemocytes in Spondylus sp.

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 15

相关文章 5

Metrics

本文评价

推荐阅读 0

[1]	张翔宇, 宋菁晨, 刘坤娜, 毛帆, 肖述, 向志明, 张扬, 喻子牛. 长牡蛎新型模式识别受体CgLRRC69的克隆和功能鉴定[J]. 热带海洋学报, 2021, 40(1): 65-74.
[2]	周晖, 陈刚, 邓崇新. 杂交石斑鱼Epinephelus fuscoguttatus♀×E. lanceolatus♂血细胞的类型及发生[J]. 热带海洋学报, 2017, 36(5): 49-58.
[3]	王旭雷, 王广策, 夏邦美. 我国西沙群岛石花菜属一新纪录变种: 匍匐石花菜扁平变种 Gelidium pusillum var. pacificum Taylor[J]. 热带海洋学报, 2016, 35(2): 76-82.
[4]	李长玲,曹伏君,黄翔鹄,刘楚吾,吴健君,刘少军. 绿海龟外周血细胞的超微结构[J]. 热带海洋学报, 2010, 29(5): 168-173.
[5]	王江勇,郭志勋,冯娟,王瑞旋. 杂色鲍血细胞免疫特点及免疫功能的研究[J]. 热带海洋学报, 2010, 29(3): 71-76.