基于OSMAC策略的Streptomyces collinus SCSIO YQ03菌株抗菌活性次级代谢产物的发现

doi:10.11978/2026035

热带海洋学报

基于OSMAC策略的Streptomyces collinus SCSIO YQ03菌株抗菌活性次级代谢产物的发现

蔡鲜花^{1, 2}, 林立五^{1, 2}, 马俊泽^{2, 3}, 刘雨晴^{1, 2}, 高永强^{2, 3}, 田新朋^{1, 2}, 鞠建华^{1, 2}, 马俊英^{1, 2}

1. 广东省海洋药物重点实验室(中国科学院南海海洋研究所) 广州 510301

2. 中国科学院大学, 北京 100049

3. 中国科学院微生物研究所，北京 100101

收稿日期:2026-02-11 修回日期:2026-04-20 接受日期:2026-04-27
通讯作者: 马俊英
基金资助:
国家自然科学基金(U23A20107); 广东省基础与应用基础研究基金(2023B1515120053)

Discovery of antimicrobial secondary metabolites from Streptomyces collinus SCSIO YQ03 based on OSMAC strategy

CAI Xianhua^{1, 2}, LIN Liwu^{1, 2}, MA Junze^{2, 3}, LIU Yuqing^{1, 2}, GAO Yongqiang^{2, 3}, TIAN Xinpeng^{1, 2}, JU Jianhua^{1, 2}, MA Junying^{1, 2}

1. Guangdong Key Laboratory of Marine Materia medica (South China Sea Institute of Oceanology), Guangzhou 510301, China

2. University of Chinese Academy of Sciences, Beijing 100049, China

3. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China

Received:2026-02-11 Revised:2026-04-20 Accepted:2026-04-27
Supported by:
National Natural Science Foundation of China (U23A20107); Guangdong Basic and Applied Basic Research Foundation (2023B1515120053)

摘要/Abstract

摘要： 本文旨在通过OSMAC策略挖掘广东湛江红树林来源放线菌Streptomyces collinus SCSIO YQ03次级代谢产物。采用硅胶柱层析、薄层制备色谱和高效液相色谱等多种分离技术对菌株的发酵产物进行分离纯化, 并通过核磁共振、质谱等波谱学方法, 结合化合物理化性质、文献数据对比进行结构鉴定。从其乙醇提取物中分离得到5个化合物, 分别为anthracimycin D (1)、anthracimycin (2)、β-rubromycin (3)、γ-rubromycin (4)、3′-hydroxy-β-rubromycin (5)。其中, 化合物1是由3个anthracimycin与1个Fe3+络合而形成的结构新颖的聚酮类化合物。在生物活性筛选中, 化合物1–5显示出良好的生物活性, 其中化合物1具有较为显著的炭疽杆菌抑制活性, MIC值为3.2μg·mL-1 , 化合物2则具有极强的抗炭疽杆菌的活性, MIC值低于0.032μg·mL-1, 化合物2–5具有显著抗MRSA活性, MIC值均为0.5μg·mL-1。从湛江红树林来源的放线菌S. collinus SCSIO YQ03中分离获得具有抗MRSA、抗炭疽杆菌活性的anthracimycin类可作为良好的抗感染药物先导化合物。

关键词: 海洋来源放线菌, 聚酮化合物, 抗感染, 抗炭疽杆菌

Abstract: In this study, the OSMAC strategy was applied to explore the secondary metabolites of mangrove-derived actinomycete Streptomyces collinus SCSIO YQ03 from Zhanjiang, Guangdong Province, China. The fermentation extract was subjected to successive separation and purification using silica gel column chromatography, preparative thin-layer chromatography, and high-performance liquid chromatography. Structural elucidation of the isolated compounds was accomplished by comprehensive spectroscopic analyses, including NMR and MS, in combination with physicochemical properties and comparison with reported data. Five compounds were isolated from the ethanol extract and were identified as anthracimycin D (1), anthracimycin (2), β-rubromycin (3), γ-rubromycin (4), and 3′-hydroxy-β-rubromycin (5). Notably, compound 1 is a structurally unique polyketide formed by the coordination of three anthracimycin units with one Fe3+ ion. Biological evaluation revealed that compounds 1–5 exhibited antibacterial activity. Compound 1 showed notable inhibitory activity against Bacillus anthracis with a MIC value of 3.2μg·mL-1, while compound 2 displayed potent anti-anthrax activity with a MIC value of <0.032μg·mL-1. In addition, compounds 3–5 demonstrated pronounced activity against MRSA. These findings indicate that anthracimycin derivatives obtained from the mangrove-derived strain S. collinus SCSIO YQ03 represent promising lead compounds for the development of anti-infective agents targeting MRSA and Bacillus anthracis.

Key words: marine actinomycetes, polyketide compounds, anti-infective, anti-Bacillus anthracis

蔡鲜花马俊泽林立五刘雨晴田新朋高永强鞠建华马俊英. 基于OSMAC策略的Streptomyces collinus SCSIO YQ03菌株抗菌活性次级代谢产物的发现[J]. 热带海洋学报, 0, (): 1-.

CAI Xianhua LIN Liwu MA Junze LIU Yuqing GAO Yongqiang TIAN Xinpeng JU Jianhua MA Junying. Discovery of antimicrobial secondary metabolites from Streptomyces collinus SCSIO YQ03 based on OSMAC strategy[J]. Journal of Tropical Oceanography, 0, (): 1-.