信号芋螺来源真菌Parengyodontium album HD005中JBIR-99的分离、抗肿瘤活性评价及培养条件筛选

doi:10.11978/2026134

热带海洋学报

信号芋螺来源真菌Parengyodontium album HD005中JBIR-99的分离、抗肿瘤活性评价及培养条件筛选

王婧, 黄金龙, 冉启臣, 秋子怡, 董帅

热带生物资源教育部重点实验室, 海南大学药学院, 海南海口 570228

收稿日期:2026-08-26 修回日期:2026-10-27 接受日期:2026-10-30
通讯作者: 董帅
基金资助:
海南省自然科学基金((823MS031))

Isolation, Antitumor Activity, and Culture-Condition Screening of JBIR-99 from the Conus litteratus-Associated Fungus Parengyodontium album HD005

WANG Jing, HUANG Jinlong, RAN Qichen, QIU Ziyi, DONG Shuai

Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Pharmaceutical Sciences, Hainan University, Haikou 570228, China

Received:2026-08-26 Revised:2026-10-27 Accepted:2026-10-30
Supported by:
Natural Science Foundation of Hainan Province(823MS031)

摘要/Abstract

摘要： 为拓展JBIR-99的海洋相关真菌来源, 评价其体外抗肿瘤活性并考察不同培养条件对其相对积累水平的影响, 对信号芋螺来源真菌 Parengyodontium album HD005进行固体发酵并对JBIR-99进行分离纯化和结构鉴定。共分离获得578.1mg JBIR-99, 经波谱分析及单晶X射线衍射确认其结构和绝对构型。体外活性评价结果表明, JBIR-99对鼻咽癌细胞CNE1、CNE2及口腔鳞状细胞癌SCC9均表现出微摩尔水平的增殖抑制活性, 而对人永生化角质形成细胞HaCaT的作用相对较弱, 其对CNE1、CNE2和SCC9的选择性指数(selectivity index, SI)分别为8.73、18.06和12.60, 表现出一定的肿瘤细胞选择性。进一步比较不同培养条件下JBIR-99的相对积累水平, 结果显示, 添加2%酵母膏和2%玉米浆后, 其相对积累水平分别达到基础培养组的2.97倍和2.72倍, 表现出较好的促进作用。研究结果扩展了JBIR-99的海洋相关真菌来源及其体外抗肿瘤活性信息, 并筛选出有利于其相对积累的培养条件, 可为JBIR-99的物质获取及后续生物活性研究提供实验依据。

关键词: 信号芋螺来源真菌, Parengyodontium album, JBIR-99, 抗肿瘤活性, 培养条件, 相对积累水平

Abstract: To expand the marine-associated fungal sources of JBIR-99, evaluate its in vitro antitumor activity, and investigate the effects of different culture conditions on its relative accumulation, the Conus litteratus-associated fungus Parengyodontium album HD005 was subjected to solid-state fermentation, followed by the isolation, purification, and structural characterization of JBIR-99. A total of 578.1mg of JBIR-99 was isolated, and its structure and absolute configuration were confirmed by spectroscopic analyses and single-crystal X-ray diffraction. In vitro activity assays showed that JBIR-99 exhibited micromolar antiproliferative activity against the nasopharyngeal carcinoma cell lines CNE1 and CNE2 and the oral squamous cell carcinoma cell line SCC9, while showing weaker cytotoxicity toward the immortalized human keratinocyte cell line HaCaT. The selectivity indices (SI) for CNE1, CNE2, and SCC9 were 8.73, 18.06, and 12.60, respectively, indicating a certain degree of tumor-cell selectivity. Further comparison of the relative accumulation of JBIR-99 under different culture conditions showed that supplementation with 2% yeast extract and 2% corn steep liquor increased its relative accumulation to 2.97- and 2.72-fold that of the basal medium, respectively, indicating favorable effects on JBIR-99 accumulation. These findings expand the marine-associated fungal sources of JBIR-99 and provide additional information on its in vitro antitumor activity, while identifying culture conditions favorable for its relative accumulation, thereby providing an experimental basis for improving JBIR-99 availability and supporting further biological activity studies.

Key words: Fungus isolated from Conus litteratus, Parengyodontium album, JBIR-99, antitumor activity, culture conditions, relative accumulation

王婧黄金龙冉启臣秋子怡董帅. 信号芋螺来源真菌Parengyodontium album HD005中JBIR-99的分离、抗肿瘤活性评价及培养条件筛选[J]. 热带海洋学报, 0, (): 1-.

WANG Jing HUANG Jinlong RAN Qichen QIU Ziyi DONG Shuai. Isolation, Antitumor Activity, and Culture-Condition Screening of JBIR-99 from the Conus litteratus-Associated Fungus Parengyodontium album HD005[J]. Journal of Tropical Oceanography, 0, (): 1-.