海洋来源Mesorhizobium sp. SCSIO 43976促进低结晶度PET降解及其多组学响应

doi:10.11978/2026142

热带海洋学报

海洋来源Mesorhizobium sp. SCSIO 43976促进低结晶度PET降解及其多组学响应

刘付敏^{1, 2}, 孙翠慈¹, 田新朋¹, 殷建平¹, 陈蕾⁴, 梁童茵^{1, 3}, 张煜航¹, 孙红岩², 董俊德¹, 凌娟¹

1. 中国科学院南海海洋研究所, 热带海洋环境与岛礁生态全国重点实验室, 广东省应用海洋生物学重点实验室, 广东广州 510301
2. 华南农业大学海洋学院, 广东广州 510642

3. 中国科学院大学, 北京 100049

4. 交通运输部水运科学研究院, 北京 100088

收稿日期:2026-09-17 修回日期:2026-10-28
通讯作者: 凌娟
基金资助:
国家重点研发计划项目(2023YFC3905003); 国家自然科学基金(42073078); 广东省科技计划项目(2026B1212170023)

Marine-derived Mesorhizobium sp. SCSIO 43976 Facilitates Degradation of Low-Crystallinity PET and Its Multi-omics Response

LIU Fumin^{1, 2}, SUN Cuici¹, TIAN Xinpeng¹, YIN Jianping¹, CHEN Lei⁴, LIANG Tongyin^{1, 3}, ZHANG Yuhang¹, SUN Hongyan², DONG Junde¹, LING Juan¹

1.State Key Laboratory of Tropical Oceanography, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2.College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China

3.University of Chinese Academy of Sciences, Beijing 100049, China

4. China Waterborne Transport Research Institute, Beijing 100088, China

Received:2026-09-17 Revised:2026-10-28
Contact: Ling, Juan
Supported by:
National Key Research and Development Program of China (2023YFC3905003); National Natural Science Foundation of China (42073078); Science and Technology Planning Project of Guangdong Province, China (2026B1212170023)

摘要/Abstract

摘要： 为探究海洋来源微生物对聚对苯二甲酸乙二醇酯(polyethylene terephthalate, PET)的生物转化特征及PET结晶度对其作用效果的影响, 从海南三亚湾海草床沉积物中分离获得菌株SCSIO 43976, 并结合16S rRNA基因序列分析进行菌株鉴定。采用熔融-低温冷淬方法对废PET瓶片进行预处理, 降低其结晶度, 设置原始PET和低结晶度PET培养体系, 经60d培养后, 通过失重率、扫描电子显微镜、凝胶渗透色谱和傅里叶变换红外光谱分析PET材料变化, 并结合转录组和非靶向代谢组解析菌株响应特征。结果表明, SCSIO 43976与中慢生根瘤菌Mesorhizobium qingshengii CCBAU 33460T的16S rRNA基因序列相似度为97.09%, 鉴定为Mesorhizobium sp. SCSIO 43976。熔融-低温冷淬处理使PET结晶度由31.75%降至7.05%。培养60d后, 低结晶度PET接菌组(T1)失重率为1.33%±0.30%, 明显高于原始PET接菌组(T2)的0.112%±0.174%; 同时T1组PET表面出现裂纹、凹陷和片层剥离, 并伴随分子量及特征化学结构变化。转录组分析显示, T1组与对照组相比获得950个差异表达基因, 主要涉及芳香族化合物降解等通路, 并检出pcaG、pcaH、pcaC及glcD、glcE、glcF、glcB等分别参与芳香族化合物转化和乙二醇代谢的功能基因。代谢组分析显示, T1与T2间筛得129个差异代谢物, 主要涉及多环芳烃降解等相关通路, 并检出与对苯二甲酸转化相关的代谢物C06318及乙二醇代谢产物1,4-二氧六环-2,5-二醇。结果表明, Mesorhizobium sp. SCSIO 43976具有废PET瓶片生物转化潜力, 降低PET结晶度可能其介导的材料转化及相关产物代谢, 为低结晶度预处理强化PET微生物转化提供了依据。

关键词: 聚对苯二甲酸乙二醇酯, PET生物降解, Mesorhizobium sp., 熔融-低温冷淬, 转录组学, 非靶向代谢组学

Abstract: To investigate the biotransformation characteristics of polyethylene terephthalate (PET) by marine-derived microorganisms and the effect of PET crystallinity on degradation efficiency, strain SCSIO 43976 was isolated from seagrass bed sediments in Sanya Bay, Hainan, and identified using 16S rRNA gene sequence analysis. Waste PET bottle flakes were pretreated by melt-quenching to reduce crystallinity. Two culture systems were established with original PET and low-crystallinity PET, respectively. After 60 days of incubation, changes in PET materials were analyzed by weight loss measurement, scanning electron microscopy (SEM), gel permeation chromatography (GPC), and Fourier-transform infrared spectroscopy (FTIR). Transcriptomics and untargeted metabolomics were employed to elucidate the bacterial response mechanisms. Results showed that strain SCSIO 43976 shared 97.09% 16S rRNA gene sequence similarity with Mesorhizobium qingshengii CCBAU 33460T and was identified as Mesorhizobium sp. SCSIO 43976. Melt-quenching treatment reduced PET crystallinity from 31.75% to 7.05%. After 60 days of cultivation, the weight loss rate of the low-crystallinity PET inoculated group (T1) reached 1.33% ± 0.30%, significantly higher than that of the original PET inoculated group (T2) at 0.112% ± 0.174%. Meanwhile, the T1 group exhibited surface cracks, pits, and lamellar exfoliation on PET films, accompanied by changes in molecular weight and characteristic chemical structures. Transcriptomic analysis revealed 950 differentially expressed genes (DEGs) between the T1 group and the control group, primarily enriched in aromatic compound degradation pathways. Functional genes including pcaG, pcaH, pcaC (involved in aromatic compound transformation) and glcD, glcE, glcF, glcB (involved in ethylene glycol metabolism) were detected. Metabolomic analysis identified 129 differential metabolites between T1 and T2 groups, mainly associated with polycyclic aromatic hydrocarbon degradation pathways. Metabolites related to terephthalic acid transformation (C06318) and ethylene glycol metabolic product (1,4-dioxane-2,5-diol) were also detected. These findings demonstrate that Mesorhizobium sp. SCSIO 43976 possesses biotransformation potential for waste PET bottle flakes, and reducing PET crystallinity can enhance strain-mediated material transformation and related product metabolism, providing evidence for enhancing microbial PET conversion through low-crystallinity pretreatment strategies.

Key words: polyethylene terephthalate, PET biodegradation, Mesorhizobium sp., melt-quenching, transcriptomics, untargeted metabolomics

刘付敏孙翠慈田新朋殷建平陈蕾梁童茵张煜航孙红岩董俊德凌娟. 海洋来源Mesorhizobium sp. SCSIO 43976促进低结晶度PET降解及其多组学响应[J]. 热带海洋学报, 0, (): 1-.

LIU Fumin SUN Cuici TIAN Xinpeng YIN Jianping CHEN Lei LIANG Tongyin ZHANG Yuhang SUN Hongyan DONG Junde LING Juan. Marine-derived Mesorhizobium sp. SCSIO 43976 Facilitates Degradation of Low-Crystallinity PET and Its Multi-omics Response[J]. Journal of Tropical Oceanography, 0, (): 1-.