一株微微型浮游植物的分离培养与鉴定

doi:10.11978/j.issn.1009-5470.2011.01.086

热带海洋学报 ›› 2011, Vol. 30 ›› Issue (1): 86-90.doi: 10.11978/j.issn.1009-5470.2011.01.086cstr: 32234.14.j.issn.1009-5470.2011.01.086

一株微微型浮游植物的分离培养与鉴定

左云龙1,2, 向文洲1, 刘纪化1,2, 苏娇娇1,2, 何慧1, 张立1, 肖伟1

1.中国科学院南海海洋研究所, 广东广州 510301; 2.中国科学院研究生院, 北京 100039

收稿日期:2009-01-16 修回日期:2010-05-14 出版日期:2011-03-17 发布日期:2011-03-16
通讯作者: 向文洲。E-mail: xwz@scsio.ac.cn
作者简介:左云龙(1982—), 男, 山东省郯城县人, 硕士研究生, 从事微藻生物技术研究。
基金资助:
广东省科技计划项目(2009B080701093); 广东省海洋与渔业局科技专项项目(A2008009-08)

Isolation, culture, and identification of a picophytoplankton

ZUO Yun-long1,2, XIANG Wen-zhou1, LIU Ji-hua1,2, SU Jiao-jiao1,2, HE Hui1, ZHANG Li1, XIAO Wei1

1. South China Sea institute of Oceanology, CAS, Guangzhou 510301, China; 2. Graduate University of CAS, Beijing 100039, China

Received:2009-01-16 Revised:2010-05-14 Online:2011-03-17 Published:2011-03-16
Contact: 向文洲。E-mail: xwz@scsio.ac.cn
About author:左云龙(1982—), 男, 山东省郯城县人, 硕士研究生, 从事微藻生物技术研究。
Supported by:
广东省科技计划项目(2009B080701093); 广东省海洋与渔业局科技专项项目(A2008009-08)

摘要/Abstract

摘要：

从我国南海北部大洋陆架外海域中取样分离培养的1株微微型浮游植物(SCSIO-8), 以它为材料, 对其生长特征、形态结构、生理生化特性和分类鉴定等进行了研究。研究结果表明, SCSIO-8为游离单细胞, 细胞大小为 (2.08±0.28µm)×(1.78±0.09)µm, 无鞭毛, 无蛋白核, 以似亲孢子方式繁殖, 形成2、4个子细胞; 扫描电镜观察, SCSIO-8具有1个细胞核、1个线粒体和1个叶绿体, 形成大量淀粉粒; SCSIO-8适应生长的盐度范围至少为0‰―38‰, 并可快速适应海淡水之间的转换; HPLC分析显示SCSIO-8细胞含有叶绿素a、b以及顺/反式紫黄质、?-胡萝卜素和叶黄素等类胡萝卜素组分; 经18S rDNA测序和对比分析, 结合其细胞形态结构、生理生化特性, 初步鉴定为Picochlorum sp. SCSIO-8。

关键词: 微微型浮游植物, Picochlorum属, 分类鉴定, 18S rDNA

Abstract:

In this study, strain SCSIO-8, an isolated picophytoplankton was obtained from the continental shelf of the northern South China Sea. Studies were carried out on its growth, morphological structure, physiological and biochemical characteris-tics and taxonomic identification. The results indicate that SCSIO-8 was a free unicellular alga without flagellate, (2.08±0.28)×(1.78±0.09)µm in size, and no pyrenoid, 1 chloroplast, 1 mitochondria, 1 nucleus, and many starch granules were observed in its cell. It was revealed that SCSIO-8 reproduced through autosporation. Results also indicate that SCSIO-8 was able to grow in a wide range of salinity from 0 to 38‰ at least, and adapted rapidly to the mutual switch between seawater and freshwater. HPLC chromatogram analysis showed that the main photosynthetic pigments of SCSIO-8 were chlorophyll a, chlorophyll b, and several carotenoids including cis- and trans-Violaxanthin, Lutein, β-carotene, etc.18S rDNA region of SCSIO-8 genome DNA was sequenced and blasted in GenBank. The picophytoplankton strain could be identified as Pi-cochlorum sp. SCSIO-8 through integrated analysis of its morphological, physiological, biochemical, and molecular charac-teristics.

Key words: picophytoplankton, Picochlorum, taxonomic identification, 18S rDNA

左云龙,向文洲,刘纪化,苏娇娇,何慧,张立,肖伟. 一株微微型浮游植物的分离培养与鉴定[J]. 热带海洋学报, 2011, 30(1): 86-90.

ZUO Yun-long,XIANG Wen-zhou,LIU Ji-hua,SU Jiao-jiao,HE Hui,ZHANG. Isolation, culture, and identification of a picophytoplankton[J]. Journal of Tropical Oceanography, 2011, 30(1): 86-90.

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一株微微型浮游植物的分离培养与鉴定

Isolation, culture, and identification of a picophytoplankton

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