三种紫菜叶状体对高温胁迫的生理响应

doi:10.11978/j.issn.1009-5470.2015.01.007

热带海洋学报 ›› 2015, Vol. 34 ›› Issue (1): 49-55.doi: 10.11978/j.issn.1009-5470.2015.01.007CSTR: 32234.14.j.issn.1009-5470.2015.01.007

三种紫菜叶状体对高温胁迫的生理响应

陈伟洲¹, 许俊宾¹, 吴文婷¹, 姜红霞², 朱建一², 陆勤勤³

1. 汕头大学海洋生物研究所, 广东省海洋生物技术重点实验室, 广东汕头, 515063;
2. 常熟理工学院, 江苏常熟, 215500;
3. 江苏省海洋水产研究所, 江苏南通, 226007

收稿日期:2014-02-08 修回日期:2014-05-06 出版日期:2015-02-10 发布日期:2015-02-10
作者简介:陈伟洲(1971~), 男, 广东省汕头市人, 教授级高工, 大学本科, 从事大型海藻生物学与生态学研究。E-mail: wzchen@stu.edu.cn
基金资助:
国家自然科学基金项目(31272664); 广东省科技计划项目(2010B060201015、2012B091100394、2013B020308007)

Physiological responses of three species of Pyropia thallus to high temperature stress

CHEN Wei-zhou, XU Jun-bin, WU Wen-ting, JIANG Hong-xia, ZHU Jian-yi, LU Qin-qin

1. Marine Biology Institute, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China;
2. Changshu Institute of Technology, Changshu 215500, China;
3. Institute of Oceanology and Marine Fisheries of Jiangsu, Nantong 226007, China

Received:2014-02-08 Revised:2014-05-06 Online:2015-02-10 Published:2015-02-10

摘要/Abstract

摘要： 文章以中国南方常见的紫菜属物种长紫菜Pyropia dentata、坛紫菜P. haitanensis和皱紫菜P. crispata叶状体为研究对象, 通过测定藻体叶绿素a、类胡萝卜素、藻红蛋白和藻蓝蛋白含量, 以及光化学量子产量、总抗氧化能力和二苯基苦基苯肼(diphenyl picrylhydrazyl, DPPH)自由基清除率, 研究了这三种紫菜对高温胁迫(26、29、32℃)的生理响应。研究结果显示, 高温胁迫造成了三种紫菜色素含量的降低, 藻体外观逐步出现了发红、发白、变透明的现象; 高温导致了三种紫菜最大光化学量子产量和实际光化学量子产量的下降, 29℃和32℃下的降低更是达到了极显著水平; 长时间(>3d)的高温胁迫也导致了紫菜总抗氧化能力和DPPH自由基清除能力的降低: 32℃下, 坛紫菜和长紫菜在第3d就开始死亡, 皱紫菜也无法存活6d。相对而言, 皱紫菜耐高温能力最强, 长紫菜次之, 而坛紫菜受高温胁迫的伤害程度则最严重, 可见皱紫菜和长紫菜具有在中国南方沿海推广栽培的潜力和优势。

关键词: 紫菜, 光合色素, 光化学量子产量, 总抗氧化, DPPH自由基

Abstract: Physiological responses to high temperature stress of the thalli of Pyropia dentata, P. haitanensis and P. crispata, which were commonly distributed in South China, were studied by measuring the content of chlorophyll a, carotenoids, phycoerythrin and phycocyanin, the yield of photochemical efficiency, the capacity of total antioxidant, and the scavenging rate of diphenyl picrylhydrazyl (DPPH)radical. Results showed that the content of pigments declined due to high temperature stress, and the thalli became red, white and transparent gradually. Pyropia thallus’ photosynthetic efficiency (maximal photochemical quantum yield and actual photochemical quantum yield) decreased under high temperature stress, especially under 29℃ and 32℃. Being under high temperature stress for a long time (>3d) also resulted in decreases of total antioxidant capacity and DPPH radical scavenging rate. P. haitanensis and P. dentata began to die when they were stressed under 32℃ for three days, and P. crispata could not survive under 32℃ for six days. P. crispata had the strongest resistance to high temperature stress followed by P. dentata, while P. haitanensis was the most sensitive one. Thus, P. crispata and P. dentata had the potential and advantages in extension of cultivation in South China.

Key words: Pyropia, photosynthetic pigment, photochemical efficiency, total antioxidant capacity, DPPH radical

陈伟洲, 许俊宾, 吴文婷, 姜红霞, 朱建一, 陆勤勤. 三种紫菜叶状体对高温胁迫的生理响应[J]. 热带海洋学报, 2015, 34(1): 49-55.

CHEN Wei-zhou¹, XU Jun-bin¹, WU Wen-ting¹, JIANG Hong-xia², ZHU Jian-yi², LU Qin-qin³. Physiological responses of three species of Pyropia thallus to high temperature stress[J]. Journal of Tropical Oceanography, 2015, 34(1): 49-55.

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三种紫菜叶状体对高温胁迫的生理响应

Physiological responses of three species of Pyropia thallus to high temperature stress

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