线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究

doi:10.11978/2021074

热带海洋学报 ›› 2022, Vol. 41 ›› Issue (2): 177-188.doi: 10.11978/2021074CSTR: 32234.14.2021074

线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究

张乐乐¹(), 邹强², 田雅楠¹, 吕春晖¹, 郑诗怡¹, 姜广峻¹, 高龙坤¹, 侯玉平³, 王凯¹()

1. 鲁东大学农学院, 山东烟台 264025
2. 烟台市科技创新促进中心, 山东烟台 264003
3. 鲁东大学生命科学学院, 山东烟台 264025

收稿日期:2021-06-11 修回日期:2021-07-22 出版日期:2022-03-10 发布日期:2021-07-26
通讯作者: 王凯
作者简介:张乐乐(1998—), 女, 山东省聊城市人, 硕士研究生, 主要从事海洋动物病害研究。email: 2508408930@qq.com
基金资助:
山东省高等学校优秀青年创新团队科技计划项目(2020KJF007);烟台市科技创新发展计划项目(2020LJRC120);烟台市科技创新发展计划项目(2019CXJJ040);山东省高校科技计划项目(J18KA146);LAMB联合资助开放基金课题(LMB20200103);威海市科技发展计划项目(2017GNS10)

Dysbiosis of both structure and function of intestinal microbiota in lined seahorses (Hippocampus erectus) as response to Edwardsiella tarda infection

ZHANG Lele¹(), ZOU Qiang², TIAN Yanan¹, LÜ Chunhui¹, ZHENG Shiyi¹, JIANG Guangjun¹, GAO Longkun¹, HOU Yuping³, WANG Kai¹()

1. School of Agriculture, Ludong University, Yantai 264025, China
2. Yantai Science and Technology Innovation Promotion Center, Yantai 264003, China
3. School of Life Science, Ludong University, Yantai 264025, China

Received:2021-06-11 Revised:2021-07-22 Online:2022-03-10 Published:2021-07-26
Contact: WANG Kai
Supported by:
Program for Outstanding Youth of Colleges and Universities(2020KJF007);Yantai Foundation for Development of Science and Technology(2020LJRC120);Yantai Foundation for Development of Science and Technology(2019CXJJ040);Shandong Province Science and Technology Research Program for Colleges and Universities(J18KA146);LMM;LMB, LMM and LAMB Co-funded Open Funds of the South China Sea Institute of the Chinese Academy of Sciences(LMB20200103);Weihai Foundation for Development of Science and Technology(2017GNS10)

摘要/Abstract

摘要：

细菌性肠炎对海马养殖业影响巨大, 但病原对海马肠道菌群的具体影响尚不清楚。文章利用已分离的病原细菌 Edwardsiella tarda YT1和海马细菌性肠炎模型, 结合16S rDNA高通量测序技术, 探究病原细菌侵染对海马肠道菌群的影响。结果发现, E. tarda侵染改变了海马肠道菌群的结构组成、多样性和丰度, 并显著降低了其多样性(p<0.05); 显著增加了海马肠道变形菌门(Proteobacteria)的相对丰度(p<0.05), 减少了放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)的相对丰度(p<0.05); 导致致病菌爱德华氏菌属(Edwardsiella)在属水平的相对丰度极显著增加(p<0.01), 而肠道固有菌群嗜冷杆菌属(Psychrobacter)和罗氏菌属(Rothia)极显著减少(p<0.01), 以及球菌属(Macrococcus)与动球菌属(Planococcus)显著减少(p<0.05)。研究结果表明, E. tarda能通过改变海马肠道固有优势菌群的相对丰度导致菌群失调。菌群功能变化及其相关性分析表明, E. tarda可能通过显著提高细菌趋化性、鞭毛组装、ABC转运蛋白、磷酸转运酶系统以及脂多糖生物合成途径的活性(p<0.05), 抑制肠道核心菌群如嗜冷杆菌属、动球菌属和谷氨酸杆菌属的丰度及其核糖体、RNA降解、核苷酸剪切修复与脂肪酸生物合成途径的活性(p<0.05), 导致肠道菌群功能失调, 并诱发肠炎。

关键词: 海马, 肠道菌群, 肠炎, 失调, 迟钝爱德华氏菌

Abstract:

Bacterial enteritis can cause severe damage to seahorse aquaculture, while little is known about the effects of bacterial pathogen infection on intestinal microbiota of seahorses. In the present study, both bacterial pathogen (Edwardsiella tarda YT1) and seahorse research model of bacterial enteritis previously reported by us were employed to explore the role of bacterial pathogen in intestinal microbiota by high-throughput full-length 16S rRNA gene sequencing. The results showed that E. tarda infection significantly altered the composition and abundance, and significantly decreased diversity of intestinal microbiota of lined seahorses (p<0.05); significantly increased the relative abundance of Proteobacteria (p<0.05), and decreased the abundance of Actinobacteria, Firmicutes, and Bacteroidetes (p<0.05); significantly increased the relative abundance of pathogenic Edwardsiella (p<0.01), while decreasing that of Psychrobacter, Rothia, Macrococcus, and Planococcus (p<0.05) at genera level. It indicates that E. tarda infection can reduce the diversity and relative abundance of intestinal autochthonous microbiota and result in dysbiosis in lined seahorses. Based on the results of bacterial function and correlation, increasing relatively abundance of E. tarda may significantly upregulate its activities of bacterial chemotaxis, flagella assembly, ABC transporter, phosphotransferase system, and lipopolysaccharide biosynthesis pathways (p<0.05), cause decrease of the relative abundance of the core intestinal microbiota of Psychrobacter, Planococcus and Glutamicibacter and suppression of their functional activities of ribosome, RNA degradation, nucleotide excision repair, and fatty acid biosynthesis pathways (p<0.05), induce dysbiosis of intestinal microbiota, and finally result in enteritis. These results may be helpful for further revealing pathogenic mechanism of E. tarda-induced enteritis in seahorses by more detailed metagenomic and metabolomics analysis.

Key words: seahorse, intestinal microbiota, enteritis, dysbiosis, Edwardsiella tarda

中图分类号:

P735.51

张乐乐, 邹强, 田雅楠, 吕春晖, 郑诗怡, 姜广峻, 高龙坤, 侯玉平, 王凯. 线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究[J]. 热带海洋学报, 2022, 41(2): 177-188.

ZHANG Lele, ZOU Qiang, TIAN Yanan, LÜ Chunhui, ZHENG Shiyi, JIANG Guangjun, GAO Longkun, HOU Yuping, WANG Kai. Dysbiosis of both structure and function of intestinal microbiota in lined seahorses (Hippocampus erectus) as response to Edwardsiella tarda infection[J]. Journal of Tropical Oceanography, 2022, 41(2): 177-188.

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样本名称	序列数/bp	碱基数/bp	平均长度/bp	最短序列长度/bp	最长序列长度/bp
Con_1	40963	17215829	420.28	211	478
Con_2	89292	37497350	419.94	208	527
Con_3	53207	22541120	423.65	211	524
ET_1	31899	13629173	427.26	211	432
ET_2	48727	20886268	428.64	211	431
ET_3	36722	15739755	428.62	319	430

名称	Con	ET	p值
Shannon指数	2.59±0.65^a	0.89±0.53^b	0.02
Simpson指数	0.19±0.06^b	0.68±0.28^a	0.04
ACE指数	449.82±31.96	362.46±232.13	0.55
Chao指数	439.46±41.06	326.82±245.5	0.47
物种覆盖度	0.99	0.99	0.39

线纹海马肠道菌群结构与功能对迟钝爱德华氏菌(Edwardsiella tarda)感染的响应特征研究

Dysbiosis of both structure and function of intestinal microbiota in lined seahorses (Hippocampus erectus) as response to Edwardsiella tarda infection

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