线纹海马(Hippocampus erectus)性腺特异性结构与生殖细胞发育特征研究 *

doi:10.11978/2020014

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (6): 93-102.doi: 10.11978/2020014CSTR: 32234.14.2020014

线纹海马(Hippocampus erectus)性腺特异性结构与生殖细胞发育特征研究 ^*

陈灵珍^1,²(), 秦耿^1,³, 王信¹, 刘雅莉¹, 肖旺红^1,², 林强^1,^2,³()

1.中国科学院热带海洋生物资源与生态重点实验室, 中国科学院南海海洋研究所, 广东广州 51030
2.中国科学院大学, 北京 100049
3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458

收稿日期:2020-02-02 修回日期:2020-03-11 出版日期:2020-10-10 发布日期:2020-03-11
通讯作者: 林强
作者简介:陈灵珍(1993—), 广东省广州市人, 硕士, 从事海洋鱼类生殖生理与发育生物学研究。email: chenlingzhen17@mails.ucas.ac.cn
基金资助:
国家重点研发计划(2016YFC1403003);南方海洋科学与工程广东省实验室(广州)重大专项团队项目(GML2019ZD0407);广东省自然科学基金(2017A030313249)

Distinct structures of gonads and germ cell development of lined seahorse, Hippocampus erectus

CHEN Lingzhen^1,²(), QIN Geng^1,³, WANG Xin¹, LIU Yali¹, XIAO Wanghong^1,², LIN Qiang^1,^2,³()

1. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Gunagzhou 511458

Received:2020-02-02 Revised:2020-03-11 Online:2020-10-10 Published:2020-03-11
Contact: LIN Qiang
Supported by:
the National Key Research and Development Program(2016YFC1403003);Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)(GML2019ZD0407);Natural Science Foundation of Guangdong Province(2017A030313249)

摘要/Abstract

摘要：

海马属(Hippocampus)鱼类具有独特的雄性育儿繁殖方式, 其卵巢和精巢结构也极其独特。本研究系统阐明了线纹海马(Hippocampus erectus)的性腺及生殖细胞的发育特征, 根据组织学结构和细胞形态学变化特征, 将线纹海马的精巢和卵巢发育过程均分为6个时期。其中, 卵巢发育方式为不同步成熟型, Ⅰ期卵巢只包含第Ⅰ时相卵母细胞, 包括卵原细胞和早期初级卵母细胞(d<20μm); Ⅱ期卵巢开始出现第Ⅱ时相卵母细胞(d=20~140μm); Ⅲ期卵巢卵母细胞数量显著增多, 体积增大, 并开始出现第Ⅲ时相卵母细胞(d=140~260μm), 此时细胞内开始出现皮质小泡、卵黄颗粒和卵黄膜; Ⅳ期卵巢开始出现第 Ⅳ时相卵母细胞(d=260~1100μm), 卵黄颗粒充满整个细胞质, 形成过渡期的卵黄球; Ⅴ期卵巢出现大量第Ⅴ时相成熟卵母细胞(d=1100~2000μm), 细胞内形成流动性卵黄囊; Ⅵ期卵巢为排卵不久后的卵巢, 卵母细胞数量显著减少。线纹海马精巢属于非限制型小叶精巢, Ⅰ期精巢主要含精原细胞, 核大, 含一到多个核仁; Ⅱ期精巢开始形成精小囊, 囊内出现初级精母细胞; Ⅲ期精巢呈乳白色, 开始出现次级精母细胞; Ⅳ期精巢体积较 Ⅲ期时显著增大, 开始出现精子细胞, 并逐渐移向中央生精腔; Ⅴ期精巢内含大量的成熟精子, 随时准备释放。精子释放后精巢进入Ⅵ期, 残留少量精细胞在生精腔中。组织化学反应结果显示, 氨基酸、蛋白质和脂肪的信号在卵母细胞的细胞质、卵黄囊中呈阳性, 且随着卵母细胞的发育而增强。本研究结果为进一步探索海马属鱼类雄性繁殖策略提供了重要的基础数据。

关键词: 线纹海马, 性腺发育, 组织结构, 卵巢, 精巢

Abstract:

Seahorses (Genus Hippocampus) have not only peculiar reproductive strategy of male pregnancy but also unique ovarian and testis structures. Developmental characteristics of the ovary and testis as well as the germ cells of the lined seahorse (Hippocampus erectus) were clarified in the present study. Based on the histological structure and morphological features, we described six stages of gonadal development of lined seahorse. Seahorses have an asynchronous ovarian developmental characteristic. The stageⅠovary contained only oogonia and early meiotic oocytes (d<20 μm). In the stage Ⅱ ovary, phase Ⅱ oocytes (d=20-140 μm) began to appear. In the stage Ⅲ ovary, the number and volume of the phase Ⅲ oocytes (d=140-260 μm) were increased remarkably. The vitellogenic oocytes (d=260-1100 μm), in which transitional yolk sphere was accumulated, could be observed in the stage Ⅳ ovary. Phase Ⅴ maturational oocytes (d=1100-2000 μm), which were characterized by highly compact vitelline envelope and fluid yolk mass, were observed in the stage Ⅴ ovary. The ovary shortly after ovulation with decrement of the number of oocytes was stage Ⅵ ovary. The testis of the lined seahorse is of the unrestricted lobular type. Stage Ⅰ testis mainly contained spermatogonia with large nuclear and one or more nucleolus. In the stage Ⅱ testis, the spermatocyst began to appear, inside of which were the primary spermatocytes with smaller nuclear. The secondary spermatocytes were first present in the stage Ⅲ testis. In the stage Ⅳ testis, the volume of testis was increased significantly and the matured spermatids appeared. Large scale of mature spermatozoa was restricted into the testicular lumen and ready to be released in the stage Ⅴ testis. The stage Ⅵ testis was vascularized with few spermatozoa or spermatocytes in the lumen. Further histochemical analysis showed that the amino acid and fat were positive in the cytoplasm and yolk mass of oocytes, and accumulated with the oocytes development. Generally, our results provide basic evidence for further understanding of male pregnancy strategies of seahorses.

Key words: Hippocampus erectus, gonad development, histological structure, ovary, testis

中图分类号:

Q953

陈灵珍, 秦耿, 王信, 刘雅莉, 肖旺红, 林强. 线纹海马(Hippocampus erectus)性腺特异性结构与生殖细胞发育特征研究 ^*[J]. 热带海洋学报, 2020, 39(6): 93-102.

CHEN Lingzhen, QIN Geng, WANG Xin, LIU Yali, XIAO Wanghong, LIN Qiang. Distinct structures of gonads and germ cell development of lined seahorse, Hippocampus erectus[J]. Journal of Tropical Oceanography, 2020, 39(6): 93-102.

图/表 4

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生长时期/d	体重/g	体长/cm	卵巢	精巢
14	0.03±0.01	2.20±0.13	性腺不可见	性腺不可见
21	0.04±0.01	2.59±0.10	肉眼可见	肉眼不可见
30	0.15±0.02	3.37±0.74	肉眼可见	肉眼可见
40	0.33±0.14	4.52±0.70	性腺细丝状, 透明, 组织切片可辨	性腺细丝状, 透明, 组织切片可辨
55	0.41±0.07	5.11±0.41	肉粉色, 性腺增粗, 细丝状, 肉眼可辨	透明, 性腺增长, 细丝状, 肉眼可辨
70	0.55±0.08	5.55±0.20	肉粉色至浅黄色, 性腺带状, 较精巢略短	透明, 性腺增长, 带状
90	2.14±0.73	8.36±0.93	淡橘色, 性腺增粗, 圆柱状, 较短, 含少量卵黄颗粒	乳白色, 性腺增粗增长, 管状, 结缔组织增多
120	4.42±0.80	9.75±1.93	橘色, 性腺增大, 蛹状, 卵黄颗粒增多, 但不能分离	乳白色, 性腺进一步增长、增粗, 管状, 结缔组织增厚
150	5.08±1.74	10.01±0.77	深橘色, 性腺体积进一步增大, 蛹状, 卵黄颗粒增多, 饱满	乳白色, 性腺依然在增粗、增长, 长管状, 结缔组织增厚, 肉眼可见血管
180	9.67±1.28	13.58±0.65	橘红色, 性腺体积进一步增大, 蛹状, 卵粒饱满, 卵子可从卵巢中挤出	乳白色, 性腺圆管状, 较粗, 精液可从精巢中挤出, 肉眼可见血管
250	10.30±0.19	13.80±0.22	深橘红色, 性腺体积变小, 松弛, 含皱缩空泡, 含卵黄颗粒, 但不能分离	乳白色, 性腺圆管状, 较粗, 肉眼可见血管

线纹海马(Hippocampus erectus)性腺特异性结构与生殖细胞发育特征研究 ^*

Distinct structures of gonads and germ cell development of lined seahorse, Hippocampus erectus

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