热带海洋学报 ›› 2012, Vol. 31 ›› Issue (1): 67-71.doi: 10.11978/j.issn.1009-5470.2012.01.067cstr: 32234.14.j.issn.1009-5470.2012.01.067

• 海洋生物学 • 上一篇    下一篇

海萝种藻冷冻保存对其释放孢子量及孢子附着的影响

陈素文 , 吴进锋 , 陈利雄   

  1. 中国水产科学研究院南海水产研究所 , 广东 广州 510300
  • 收稿日期:2010-11-02 修回日期:2011-02-14 出版日期:2012-03-10 发布日期:2012-03-13
  • 通讯作者: 陈素文
  • 作者简介: 陈素文 (1968 — ), 女 , 广东省揭阳市人 , 研究员 , 从事海水贝藻育苗及养殖的研究。 E-mail chensuwen407@163.com , Tel 020-84451432
  • 基金资助:
    广东省科技计划项目 (2006B20201061); 广东省海洋渔业科技推广专项 (A200899E01); 中国水产科学研究院南海水产研究所中央级公益性科研院所专项资金项目 (2007TS01, 2010TS02)

Effects of freezing storage of fertile Gloiopeltis furcata on its released spores’ number and spores’ attachment

CHEN Su-wen, WU Jin-fen g , CHEN Li-xiong   

  1. South China Sea Fisheries Research Institute , Chinese Academy of Fishery Sciences , Guangzhou 510300, China
  • Received:2010-11-02 Revised:2011-02-14 Online:2012-03-10 Published:2012-03-13

摘要: 为了研究海萝的非繁殖季节孢子育苗方法, 进行了2个实验来研究海萝种藻冷冻保存对其释放孢子量及孢子附着的影响。1)6组不同风干程度海萝种藻在-18℃冷冻保存6个月后取出在16℃水中进行孢子的释放, 种藻风干到干重为原鲜重约25%的组, 孢子的保存效果最好, 该组种藻释放孢子量约为对照组(未经风干和保存的种藻)的77. 6%, 且孢子的附着率与对照组的差异性不显著; 2)风干到重量为原鲜重约1/4的种藻分别保存于-18℃、-36℃、-80℃, 在保存8个月后, 每一保存温度下的种藻分别在8℃、12℃、16℃、20℃水中释放孢子, 种藻在水温12℃下释放的孢子量最多; 保存于-36℃、-80℃的种藻较保存于-18℃的释放孢子量多。本实验结果为海萝的非繁殖季节孢子育苗找到一种简便的方法, 也为该属藻类的种质保存提供了参考。

关键词: 海萝种藻, 冷冻保存, 孢子

Abstract: In order to study spore culture of Gloiopeltis furcata in non-reproduction season, two experiments were conducted on freezing storage of fertile Gloiopeltis furcata in terms of its released spores’ number and spores’ attachment. In the first experiment, six group of fertile Gloiopeltis furcata of different dry-degree was preserved at - 18 ℃ for six months and then released at water temperature of 16 ℃ . The results showed that the best preservation effect of fertile Gloiopeltis furcata was the group that dry weight was about 25% of its fresh weight. The released spores’ number of this group was about 77.6% of the control group (the fertile Gloiopeltis furcata which was not dealed with air drying and preservation) and there was no significant difference in spores’ attachment between this group and the control group. In the second experiment, fertile Gloiopeltis furcata that dry weight was about 1/4 of its fresh weight was preserved at - 18 ℃ , - 36 ℃ , and - 80 ℃ for eight months and then released spores at water temperature of 8 ℃ , 12 ℃ , 16 ℃ , and 20 ℃ . The results showed maximum spores appeared at water temperature of 12 ℃ and fertile Gloiopeltis furcata that preserved at - 36 ℃ and - 80 ℃ released more spores than those preserved at - 18 ℃ . The two experiments showed a simple and convenient method for spore culture of Gloiopeltis furcata in non-reproduction season and would assist the preservation of germ plasm of Gloiopeltis.

Key words: fertile Gloiopeltis furcata , freezing storage, spore

中图分类号: 

  • P735.2