不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响

doi:10.11978/j.issn.1009-5470.2013.01.009

热带海洋学报 ›› 2013, Vol. 32 ›› Issue (1): 64-69.doi: 10.11978/j.issn.1009-5470.2013.01.009cstr: 32234.14.j.issn.1009-5470.2013.01.009

不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响

张敬键, 展望, 李爱芬, 张成武

暨南大学水生生物研究中心，热带亚热带水生态工程教育部工程研究中心，广东广州 510632

收稿日期:2013-04-20 修回日期:2013-04-20 出版日期:2013-04-19 发布日期:2013-04-20
通讯作者: 张成武, 男，江苏省泰州市人，教授，博士生导师。E-mail: tzhangcw@jnu.edu.cn
作者简介:张敬键(1984—)，女，山东省临沂市人，硕士研究生，从事微藻生物技术及应用研究
基金资助:
国家高技术研究发展计划重点项目(2009AA064401,2013AA065805)；广东省产学研重点项目(2010A090200008)

Effects of sodium nitrate concentration on the growth and lipid accumulation of Pseudochlorococcum polymorphum and Pseudochlorococcum typicum

ZHANG Jing-jian, ZHAN Wang, LI Ai-fen, ZHANG Cheng-wu

Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering, Ministry of Education, Guangzhou 510632, China

Received:2013-04-20 Revised:2013-04-20 Online:2013-04-19 Published:2013-04-20

摘要/Abstract

摘要： 以多形拟绿球藻(Pseudochlorococcum polymorphum UTEX 1791)和模式拟绿球藻(Pseudochlorococcum typicum UTEX 1792)为材料，采用BG-11培养基，研究其在0.3、0.5、1.0和1.5g·L^-1等4个硝酸钠浓度条件下的生长和总脂含量。结果表明：适宜多形拟绿球藻生长的硝酸钠浓度范围为0.3—1.0g·L^-1，其最终生物质干重在3.7g·L^-1左右；模式拟绿球藻在1.0g·L^-1的硝酸钠浓度条件下生长最好，可获得4.35g·L^-1的生物质干重。在培养过程中两株绿藻的叶绿素a含量与总脂的含量关系密切，在培养初期各实验组叶绿素a含量增加，藻体的总脂含量增长不明显，而在培养中期藻细胞叶绿素a的含量增长平缓，总脂含量增长较明显，其中在0.3g·L^-1的硝酸钠浓度实验组总脂增长最为显著，多形拟绿球藻增长了11.65%，模式拟绿球藻增长了24.99%。两株绿藻在0.3g·L^-1的低氮实验组总脂含量最高，多形拟绿球藻和模式拟绿球藻总脂分别为细胞干重的44.35%和26.28%，在此条件下两株藻的最大总脂收获量分别为0.98g·L^-1和1.61g·L^-1。

关键词: 多形拟绿球藻, 模式拟绿球藻, 硝酸钠浓度, 总脂含量, 最大总脂收获量

Abstract: The present work was oriented toward analyzing the growth and total lipid accumulation of two green microalgae, Pseudochlorococcum polymorphumUTEX 1791 and PseudochlorococcumtypicumUTEX 1792, both of which were cultured in the BG-11medium but under different sodium nitrate concentrations of 0.3, 0.5, 1.0, and 1.5 g·L^-1. The results showed that the sodium nitrate concentration, ranging from 0.3 to 1.0g·L^-1 was suitable for the growth of P. polymorphumUTEX 1791: its final dry biomass weight was about 3.7g·L^-1. The optimum concentration of sodium nitrate for P.typicumUTEX 1792 was 1.0 g·L^-1, and its final dry biomass weight reached 4.35g·L^-1. The close relationship between the variation of chlorophyll a content and the accumulation of total lipid was observed from these two algal cultures. At the early growth phase, the content of chlorophyll ain all experimental groups increased sharply, while the total lipid accumulation was not significant. However, in the mid growth phase, the content of chlorophyll aincreased slowly, but the total lipid content increased significantly in all experimental groups, especially in the 0.3g·L^-1 of sodium nitrate group, whose total lipid content increased by 11.65% (UTEX1791) and 24.99% (UTEX 1792), the maximum total lipid content and yield were up to 26.28% (dry weight)(UTEX1791) and 44.35% (dry weight) (UTEX 1792), 0.98 g·L^-1 (UTEX 1791) and 1.61 g·L^-1 (UTEX 1792), respectively.

Key words: Pseudochlorococcum polymorphum, Pseudochlorococcum typicum, sodium nitrate, total lipid content, maximum lipid yield

中图分类号:

Q945

张敬键, 展望, 李爱芬, 张成武. 不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响[J]. 热带海洋学报, 2013, 32(1): 64-69.

ZHANG Jing-jian, ZHAN Wang, LI Ai-fen, ZHANG Cheng-wu. Effects of sodium nitrate concentration on the growth and lipid accumulation of Pseudochlorococcum polymorphum and Pseudochlorococcum typicum [J]. Journal of Tropical Oceanography, 2013, 32(1): 64-69.

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不同硝酸钠浓度对多形拟绿球藻和模式拟绿球藻生长及脂类积累的影响

Effects of sodium nitrate concentration on the growth and lipid accumulation of Pseudochlorococcum polymorphum and Pseudochlorococcum typicum

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