热带海洋学报 ›› 2011, Vol. 30 ›› Issue (3): 99-104.doi: 10.11978/j.issn.1009-5470.2011.03.099

• 海洋生物学 • 上一篇    下一篇

哈维氏弧菌生物被膜体外模型的建立及成膜特性研究

李海平 1,  鄢庆枇 1,  徐晓津 1,  苏永全 2,  覃映雪 1
    

  1. 1.集美大学水产学院,  福建省高校水产科技与食品安全重点实验室,  福建  厦门 361021; 2.  厦门大学海洋与环境学院,  福建
    厦门 361005
  • 收稿日期:2010-06-29 修回日期:2011-03-28 出版日期:2011-07-18 发布日期:2011-07-20
  • 通讯作者: 覃映雪。E-mail: yxqin@jmu.edu.cn
  • 作者简介:李海平(1985—), 男, 福建省厦门市人, 硕士研究生, 主要从事水产微生物研究。
  • 基金资助:

    国家高技术研究发展计划项目(2007AA09Z115)

Establishment of in vitro biofilm model and characteristics of  biofilm formation of Vibrio harveyi

LI Hai-ping 1 , YAN Qing-pi 1 , XU Xiao-jin 1 , SU Yong-quan 2 , QIN Ying-xue 1   

  1. 1. Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and Food Safety, Xiamen 361021,
    China; 2. College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, China
  • Received:2010-06-29 Revised:2011-03-28 Online:2011-07-18 Published:2011-07-20

摘要:

采用改良微孔板法建立哈维氏弧菌 Vibrio harveyi TS-628 菌株生物被膜体外模型,  并进一步研究环境因子
对哈维氏弧菌体外形成生物被膜的影响。结果发现,  哈维氏弧菌 TS-628 菌株可以在聚苯乙烯板中形成生物被膜,
并且该菌生物被膜的形成受起始菌液浓度、温度、pH 等理化因子的显著影响。温度为 30℃、NaCl 质量分数为   
3%—6%、pH 为 7、孵育时间为 36h 是哈维氏弧菌形成生物被膜的最佳条件。此外哈维氏弧菌在分别经表皮黏液、
鳃黏液、肠道黏液、肝脏提取液、脾脏提取液、肌肉提取液包被的基质上成膜效果显著,  其中在经肝脏提取液包
被的基质上形成的生物被膜量显著高于在其他生物基质上形成的生物被膜量(p<0.01)。

关键词: 哈维氏弧菌, 生物被膜, 环境因子, 微孔板法, 生物基质

Abstract:

 Modi?ed microtiter-plate test was used to establish an in vitro biofilm model of Vibrio harveyi TS-628 strain. Effect
of environmental factors on bacterial biofilm formation in vitro was also studied. The results show that V. harveyi TS-628
strains could form biofilm in polystyrene plates, and biofilm formation of this bacterium was significantly affected by different
environmental factors, such as temperature, initial bacterial concentration, pH, NaCl concentration, and so on. The optimum
conditions for biofilm formation of  V.  h ar v e y i   TS-628 strain are at temperature of 30℃, pH=7, NaCl concentration of     
3%−6%, and using 36h incubation time. Furthermore, V. harveyi TS-628 strain could form biofilm on the walls coated with
skin mucus, gill mucus, intestinal mucus, liver extract, spleen extract, and muscle extract. The biofilm formation of V.  ha r v e y i
on the walls coated with liver extract exhibited a remarkably higher value of D(590)(p<0.01)than that on the walls coated with
other tissue extracts.

Key words: Vibrio harveyi , biofilm, environmental factor, microtiter-plate test, biological matrix