铝对海洋固氮蓝藻Crocosphaera watsonii生长及固氮速率的影响

doi:10.11978/2016069

热带海洋学报 ›› 2017, Vol. 36 ›› Issue (2): 12-18.doi: 10.11978/2016069CSTR: 32234.14.2016069

铝对海洋固氮蓝藻Crocosphaera watsonii生长及固氮速率的影响

刘甲星^1,^2,³(), 周林滨^1,², 柯志新^1,², 李刚^1,², 史荣君^1,^2,³, 谭烨辉^1,²()

1. 中国科学院南海海洋研究所中国科学院热带海洋生物资源与生态重点实验室, 广东广州 510301
2. 广东省应用海洋生物学重点实验室, 广东广州 510301
3. 中国科学院大学, 北京 100049

收稿日期:2016-07-25 修回日期:2016-08-31 出版日期:2017-03-20 发布日期:2017-04-06
作者简介:
作者简介：刘甲星(1986—), 男, 博士研究生, 主要从事海洋生态学研究。E-mail: ljx2ljx@163.com
基金资助:
国家重点基础研究发展规划(“973”计划)(2015CB4529000、2015CB452903);国家自然科学基金项目(41276162、41506150);中国科学院战略先导专项 (XDA11020200);公益性行业(农业)科研专项(201403008);广东省科技计划项目(2014B030301064)

Effect of aluminum on the growth and nitrogen fixation of a marine nitrogen-fixing cyanobacterium: Crocosphaera watsonii

Jiaxing LIU^1,^2,³(), Linbin ZHOU^1,², Zhixin KE^1,², Gang LI^1,², Rongjun SHI^1,^2,³, Yehui TAN^1,²()

1. Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
2. Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China
3. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2016-07-25 Revised:2016-08-31 Online:2017-03-20 Published:2017-04-06
Supported by:
National Program on Key Basic Research Project (“973” Program)(2015CB4529000, 2015CB452903);National Science Foundation of China (41276162, 41506150);Strategic Priority Research Program of the Chinese Academy of Sciences (XDA11020200);Special Fund for Agro-scientific Research in the Public Interest (201403008);Science and Technology Planning Project of Guangdong Province (2014B030301064)

摘要/Abstract

摘要：

大气沉降向海洋表层输入大量的铝, 然而关于铝对海洋浮游植物的影响却少有研究报道。目前, 铝对在海洋碳和氮循环中起着重要作用的固氮生物影响的研究还较少。文章以Aquil培养基(无氮硅营养盐)半连续培养单细胞海洋固氮蓝藻Crocosphaera watsonii, 利用流式细胞技术和¹⁵N同位素示踪法, 研究铝(0、0.2、2和20μmol•L^-1)对Crocosphaera watsonii生长和固氮作用的影响。数据表明, 3个铝处理组Crocosphaera watsonii细胞大小、光合色素含量和光系统Ⅱ(PSⅡ)原初光能转化效率(F_v/F_m)都显著降低, 生长速率和固氮速率明显受到抑制。结果表明, 在营养充足条件下, 铝对Crocosphaera watsonii生长和固氮具有抑制性作用。

关键词: 铝, 固氮蓝藻, Crocosphaera watsonii, 生长速率, 固氮

Abstract:

Although a large amount of aluminum enters the ocean through atmospheric dust deposition every year, the effect of aluminum on marine phytoplankton is seldom examined. Few studies focused on the effects of aluminum on marine nitrogen-fixing cyanobacteria, which play important roles in nitrogen and carbon cycling in the ocean. In this study, a unicellular marine nitrogen-fixing cyanobacterium Crocosphaera watsonii grew in Aquil medium without silicate and nitrogen by semi-continuous cultures. We studied the effect of aluminum (0, 0.2, 2 and 20 μmol•L^-1) on the growth rate and nitrogen fixation rate of Crocosphaera watsonii by using flow cytometry and ¹⁵N isotope tracer method. Results showed that aluminum inhibited the growth and nitrogen fixation of Crocosphaera watsonii. Results also showed that cell size, cellular pigments and photochemical efficiency of photosystem II (F_v/F_m) of Crocosphaera watsonii treated with aluminum were significantly decreased. These results demonstrate that aluminum has an inhibitory effect on the growth and nitrogen fixation of Crocosphaera watsonii under the condition of sufficient phosphate and trace nutrients.

Key words: aluminum, nitrogen-fixing cyanobacteria, Crocosphaera watsonii, growth rate, nitrogen fixation

中图分类号:

P735

刘甲星, 周林滨, 柯志新, 李刚, 史荣君, 谭烨辉. 铝对海洋固氮蓝藻Crocosphaera watsonii生长及固氮速率的影响[J]. 热带海洋学报, 2017, 36(2): 12-18.

Jiaxing LIU, Linbin ZHOU, Zhixin KE, Gang LI, Rongjun SHI, Yehui TAN. Effect of aluminum on the growth and nitrogen fixation of a marine nitrogen-fixing cyanobacterium: Crocosphaera watsonii[J]. Journal of Tropical Oceanography, 2017, 36(2): 12-18.

图/表 4

参考文献 44

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铝对海洋固氮蓝藻Crocosphaera watsonii生长及固氮速率的影响

Effect of aluminum on the growth and nitrogen fixation of a marine nitrogen-fixing cyanobacterium: Crocosphaera watsonii

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