雷州半岛近岸海域海洋红酵母的分离鉴定

doi:10.11978/2016117

热带海洋学报 ›› 2017, Vol. 36 ›› Issue (4): 87-92.doi: 10.11978/2016117CSTR: 32234.14.2016117

雷州半岛近岸海域海洋红酵母的分离鉴定

孙建男^1,²(), 刘影¹, 谢为天¹, 徐春厚¹()

1. 广东海洋大学, 广东湛江 524088
2. 奥健生物科技有限公司, 广东广州 510000

收稿日期:2016-11-16 修回日期:2016-12-27 出版日期:2017-07-20 发布日期:2017-07-26
作者简介:
作者简介：孙建男(1989—), 女, 辽宁省朝阳市人, 硕士, 研究方向为微生物学与分子生物学。E-mail:1042341150@qq.com
基金资助:
广东省科技计划项目(2013B020307013);广东省农业科技项目(201201147);湛江市科技计划项目(2015B01013)

Isolation and identification of marine Rhodotorula in a coastal area near Leizhou Peninsula

Jiannan SUN^1,²(), Ying LIU¹, Weitian XIE¹, Chunhou XU¹()

1. Guangdong Ocean University, Zhanjiang 524088, China
2. LIGENSIA Bio-Technology Limited Incorporation, Guangzhou 510000, China

Received:2016-11-16 Revised:2016-12-27 Online:2017-07-20 Published:2017-07-26
Supported by:
Science and Technology Program of Guangdong Province (2013B020307013);Agricultural Science and Technology Program of Guangdong Province (201201147);Science and Technology Program of Zhanjiang (2015B01013)

摘要/Abstract

摘要：

从雷州半岛近岸海域采集海洋生物、海水、海泥等样品, 共计317份。将样品处理后先富集培养, 再用红酵母琼脂培养基及麦芽汁琼脂培养基进行分离培养, 之后对分离菌株通过菌体形态、培养特性及生理生化试验结果进行初步鉴定。根据红酵母的初步鉴定结果, 选取有代表性的菌株作26s rDNA D1/D2区域序列测定, 构建系统发育树, 进行多样性分析。结果表明, 从317份样品中分离鉴定出71株海洋红酵母; 其中, 有代表性的36株红酵母归属于5个种和2个疑似新种。36株红酵母包括胶红酵母(Rhodotorula mucilaginosa)22株、粘红酵母(Rhodotorula glutinis)6株、红冬孢酵母(Rhodosporidium toruloides)3株、斯鲁菲亚红酵母(Rhodotorula slooffiae)2株、浅红红酵母1株(Rhodotorula pallida)和疑似红酵母新种2株(Rhodotorula sp, cZDJ2和Rhodotorula sp. ZTC2)。雷州半岛近岸海域的海洋红酵母优势种为胶红酵母, 种群的多样性不够丰富。

关键词: 海洋红酵母, 分离鉴定, 近岸海域, 雷州半岛

Abstract:

In this study, 317 samples from marine organisms, seawater and sea mud were gathered in a coastal area near the Leizhou Peninsula. After basic process and enrichment culture, marine Rhodotorula was isolated by using Rhodotorula agar medium and wort agar medium. The Rhodotorula strains were preliminary identified by using cell morphology characteristics, cultural characteristics, physiological and biochemical test results. Based on the preliminary identification results, representative strains were tested by 26s rDNA D1/D2 domain sequence analysis, phylogenetic tree was constructed and diversity was analyzed. The 71 strains isolated from 317 samples were preliminary identified as marine Rhodotorula among which 36 representative strains were identified as belong to five species and two suspected to be new species. Among them, 22 strains were R. mucilaginosa, six strains were R. glutinis, three strains were R. toruloides, two strains were R. slooffiae, one strain was R. pallida, and two strains were suspected to be new species named Rhodotorula sp. cZDJ2 and Rhodotorula sp. ZTC2, respectively. The dominant species of marine Rhodotorula in the coastal area near the Leizhou Peninsula was Rhodotorula mucilaginosa, indicating that the diversity of population in the coastal area near the Leizhou Peninsula was not rich enough.

Key words: Marine Rhodotorula, Isolation and Identification, Coastal area, Leizhou Peninsula

中图分类号:

Q93-331

孙建男, 刘影, 谢为天, 徐春厚. 雷州半岛近岸海域海洋红酵母的分离鉴定[J]. 热带海洋学报, 2017, 36(4): 87-92.

Jiannan SUN, Ying LIU, Weitian XIE, Chunhou XU. Isolation and identification of marine Rhodotorula in a coastal area near Leizhou Peninsula[J]. Journal of Tropical Oceanography, 2017, 36(4): 87-92.

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参考文献 16

[1]	BARNETT J A, PAYNE R W, YARROW D, 1991. 酵母菌的特征与鉴定手册[M]. 胡瑞卿, 译. 青岛: 青岛海洋大学出版社.
	BARNETT J A, PAYNE R W, YARROW D, 1991. Yeasts: characteristics and identification[M]. HU RUIQING, Trans. Qingdao: Qingdao Ocean University Press (in Chinese).
[2]	李治滢, 周新丽, 周斌, 等, 2017. 云南程海湖冬季酵母菌多样性及胞外酶活性研究[J]. 菌物学报, 36(2): 177-185.
	LI ZHIYING, ZHOU XINLI, ZHOU BIN, et al, 2017. Diversity and extracellular enzymes of yeasts from Chenghai Lake in winter[J]. Mycosystema, 36(2): 177-185 (in Chinese with English abstract).
[3]	孙建男, 谢为天, 刘颖, 等, 2015. 海洋红酵母的研究进展[J]. 安徽农业科学, 43(4): 84-88.
	SUN JIANNAN, XIE WEITIAN, LIU YING, et al, 2015. Research advances of the marine red yeast[J]. Journal of Anhui Agricultural Sciences, 43(4): 84-88 (in Chinese with English abstract).
[4]	汪洋, 孔维宝, 韩锐, 等, 2015. 红酵母简介[J]. 生物学通报, 50(4): 15-17.
[5]	魏艳敏, 钟辉, 刘钢, 等, 1997. 中国淡水红酵母(Rhodotorula Harrison)种类调查[J]. 南开大学学报(自然科学), 30(3): 103-105.
	WEI YANMIN, ZHONG HUI, LIU GANG, et al, 1997. Species of Rhodotorula Harrison from fresh water of China[J]. Acta Scientiarum Naturalium Universitatis Nankaiensis, 30(3): 103-105 (in Chinese with English abstract).
[6]	叶伟庆, 吴园园, 高上吉, 等, 2013. 4株海洋红酵母分离鉴定及其代谢产物分析[J]. 热带作物学报, 34(10): 2046-2050.
	YE WEIQING, WU YUANYUAN, GAO SHANGJI, et al, 2013. Isolation, identification and metabolites analysis of four marine Rhodotorula strains[J]. Chinese Journal of Tropical Crops, 34(10): 2046-2050 (in Chinese with English abstract).
[7]	周与良, 黄铁石, 但汉斌, 等, 1999. 中国海红酵母属(Rhodotorula Harrison)的种类[J]. 南开大学学报(自然科学), 32(4): 115-116.
	ZHOU YULIANG, HUANG TIESHI, DAN HANBIN, et al, 1999. Rhodotorula Haririson in China Sea[J]. Acta Scientiarum Naturalium Universitatis Nankaiensis, 32(4): 115-116 (in Chinese with English abstract).
[8]	CONNELL L, BARRETT A, TEMPLETON A, et al, 2009. Fungal Diversity associated with an active deep sea volcano: Vailulu'u Seamount, Samoa[J]. Geomicrobiology Journal, 26(8): 597-605.
[9]	FELL J W, BOEKHOUT T, FONSECA A, et al, 2000. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis[J]. International Journal of Systematic and Evolutionary Microbiology, 50(3): 1351-1371.
[10]	HAGLER A N, MENDONÇA-HAGLER L C, 1981. Yeasts from marine and estuarine waters with different levels of pollution in the state of Rio de Janeiro, Brazil[J]. Applied and Environmental Microbiology, 41(1): 173-178.
[11]	PRABHAKARAN N, GUPTA R, 1991. Yeasts from the sediment samples of the EEZ along the southwest coast of India[J]. Journal of the Marine Biological Association of India, 33(1-2): 455-459.
[12]	SINGH P, SINGH S M, TSUJI M, et al, 2014. Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic[J]. Cryobiology, 68(1): 122-128.
[13]	WHITE T J, BRUNS T, LEE S, et al, 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[M]//INNIS M A, GELFAND D H, SNINSKY J J, et al. PCR Protocols: A Guide to Methods and Applications. New York: Academic Press.
[14]	YAMASATO K, GOTO S, OHWADA K, et al, 1974. Yeasts from the Pacific Ocean[J]. The Journal of General and Applied Microbiology, 20(5): 289-307.
[15]	ZHANG HONGYIN, YANG QIYA, GE LINGLING, et al, 2016. Chitin enhances biocontrol of Rhodotorula mucilaginosa to postharvest decay of peaches[J]. International Journal of Biological Macromolecules, 88: 465-475.
[16]	ZHANG XUE, HUA MINGXIA, SONG CHUNLI, et al, 2012. Occurrence and diversity of marine yeasts in Antarctica environments[J]. Journal of Ocean University of China, 11(1): 70-74.

分离菌株	蔗糖	麦芽糖	海藻糖	松三糖	木糖	鼠李糖	乳糖	蜜二糖	棉子糖	甘露糖	菊糖	肌醇	淀粉	硝酸盐	亚硝酸盐	乙胺	肌酸	肌酸酐
ZTC4等46株	+	+	+	+	+	v	-	-	+	v	-	-	-	-	-	+	-	-
aZDD4等12株	+	+	+	+	v	+	-	-	+	+	+	-	-	+	+	+	-	-
ZTHD2等6株	+	+	+	+	+	-	-	-	v	v	v	-	-	+	+	v	-	-
aZDB等4株	+	-	+	+	+	v	+	-	v	v	v	-	-	+	+	-	-	-
ZZC2	+	-	+	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-
ZTC2	-	+	+	-	+	+	-	-	+	+	-	-	-	+	+	+	-	-
cZDJ2	+	-	+	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-

类群	分离菌株(Genbank登录号)	相似性最高模式菌株	相似性/%
R. mucilaginous (22株)	ZZC1(KP979796)、aZDD6(KP954615)	R. mucilaginosa ATCC 32763	99.6
	ZTHD4(KP954623、ZTHG1(KP954624)
	aZDY(KP966487)、ZTHY5(KP966488)
	ZTHQ(KP954629)
	cZDL1(KP954617)、aZDY1(KP954618)	R. mucilaginosa ATCC 32763	99.5
	aZDZ1(KP954619)、cZDY5(KP954620)
	bZDB2(KP966490)、 ZDS(KP954630)
	ZTHY1(KP954625)	R. mucilaginosa ATCC 32763	99.3
	aZDD3(KP979795)	R. mucilaginosa ATCC 32763	99.1
	cZDY2(KP954616)、ZTHD3(KP954622)	R. mucilaginosa CBS 8383	99.6
	ZTC4(KP966489)	R. mucilaginosa CBS 8383	99.5
	ZTHY4(KP954626)、ZTC3(KP954627) ZTC5(KP954628) 、ZTHY2(KP966486)	R. mucilaginosa KCTC 2829	99.6
R. glutinis(6株)	cZDD5(KP979800)、 bZDD5(KP979801) bZDD4(KP979802) 、bZDD1(KP979803) aZDD5(KP979804)、aZDD4(KP979805)	R. glutinis ATCC 32765	99.3
R. toruloides(3株)	ZTHD2(KP979806)、ZTC1(KP979807)	R. toruloides KCTC 17083	99.5
R. toruloides(3株)	ZTHD1(KP979808)	R. toruloides KCTC 17083	99.1
R. slooffiae(2株)	aZDH8(KP979798)、aZDB(KP979799)	R. slooffiae CBS 5706	99.5
R . pallid(1株)	ZZC2(KP979797)	R. pallid CBS 320	99.3
Rhodotorula sp.(1株)	cZDJ2(KP979809)	R. minuta JCM 5951	93.0
Rhodotorula sp.(1株)	ZTC2(KP954620)	R. mucilaginosa ATCC 32763	98.7

雷州半岛近岸海域海洋红酵母的分离鉴定

Isolation and identification of marine Rhodotorula in a coastal area near Leizhou Peninsula

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