热带海洋学报 ›› 2025, Vol. 44 ›› Issue (1): 146-153.doi: 10.11978/2024051CSTR: 32234.14.2024051

• 海洋资源开发 • 上一篇    下一篇

海洋本草软珊瑚共附生真菌Aspergillus terreus EGF7-0-1中γ-芳环丁烯内酯类化合物研究(I)

何悦铭1(), 赵立凝2(), 陈欣琪1, 何嘉鸿1, 樊浩1, 陈乐怡1, 张翠仙1(), 何细新1()   

  1. 1.广州中医药大学中药学院, 广东 广州 510006
    2.广州中医药大学图书馆, 广东 广州 510006
  • 收稿日期:2024-03-05 修回日期:2024-03-17 出版日期:2025-01-10 发布日期:2025-02-10
  • 通讯作者: 张翠仙, 何细新
  • 作者简介:

    何悦铭(1998—), 男, 广东江门人, 硕士研究生, email: ;

    赵立凝(1976—), 女, 河北秦皇岛人, 硕士研究生, 副教授, email:

    何悦铭、赵立凝为共同第一作者

  • 基金资助:
    国家自然科学基金项目(82273845); 广东省自然资源厅专项(GDNRC[2023]37); 广东省自然资源厅专项(GDNRC[2020]039); 广东省自然资源厅专项(GDNRC[2021]048); 广东省大学生创新创业项目(202310572224); 广东省大学生创新创业项目(202310572291)

γ-Aromatic butenolides lignans from soft coral-associated symbiotic and epiphytic fungi Aspergillus terreus EGF7-0-1(I)

HE Yueming1(), ZHAO Lining2(), CHEN Xinqi1, HE Jiahong1, FAN Hao1, CHEN Leyi1, ZHANG Cuixian1(), HE Xixin1()   

  1. 1. School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
    2. Library, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
  • Received:2024-03-05 Revised:2024-03-17 Online:2025-01-10 Published:2025-02-10
  • Contact: ZHANG Cuixian, HE Xixin
  • Supported by:
    National Natural Science Foundation of China(82273845); Special Fund of Guangdong Provincial Department of Natural Resources(GDNRC[2023]37); Special Fund of Guangdong Provincial Department of Natural Resources(GDNRC[2020]039); Special Fund of Guangdong Provincial Department of Natural Resources(GDNRC[2021]048); Innovation and Entrepreneurship Program for College Students in Guangdong Province(202310572224); Innovation and Entrepreneurship Program for College Students in Guangdong Province(202310572291)

摘要:

为了从海洋微生物中寻找结构新颖的次生代谢产物, 本文采用大米培养基对海洋本草软珊瑚共附生真菌Aspergillus terreus EGF7-0-1进行规模发酵, 运用薄层色谱、柱层析和高效液相色谱等多种色谱技术对发酵产物进行分离纯化, 通过核磁共振波谱、高分辨质谱和旋光光谱等多种光谱学方法, 并结合文献数据比对确定了8个γ-芳环丁烯内酯类化合物: butyrolactones I—V(15)、aspernolide E(6)、asperteretal D(7)和 terrusnolide A(8)。首次对化合物18进行体外抑制酪氨酰-DNA磷酸二酯酶I (tyrosyl-DNA phosphodiesterase 1, TDP1)活性测试, 均无抑制作用。本研究为TDP1抑制剂的发现提供借鉴。

关键词: 海洋本草软珊瑚, Aspergillus terreus, γ-芳环丁烯内酯类, 结构鉴定, TDP1抑制剂

Abstract:

In order to discover the novel and bioactive secondary metabolites, the soft coral-associated symbiotic and epiphytic fungi Aspergillus terreus EGF7-0-1 were fermented in rice media. The secondary metabolites were isolated using various chromatographic technologies, yielding eight γ-aromatic butenolide lignans. Based on nuclear magnetic resonance (NMR), high resolution electrospray ionization mass spectroscopy (HR-ESI-MS) and comparison with the literature, their structures were determined to be butyrolactones I−V (15), aspernolide E (6), asperteretal D (7) and terrusnolide A (8). In this study, the inhibitory activity of these compounds against TDP1 (Tyrosyl-DNA phosphodiesterase 1) in vitro was studied for the first time, but it was unfortunate that compounds 18 did not show any inhibitory abilities against TDP1. This study provides theoretical data for the discovery of TDP1 inhibitors.

Key words: traditional marine Chinese medicine soft coral, Aspergillus terreus, γ-aromatic butenolides lignans, structural identification, TDP1 inhibitors

中图分类号: 

  • O629