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热带海洋学报

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一株珊瑚来源二苯甲酮-3降解菌Sphingopyxis terrae SCSIO 90395的分离鉴定及基因组特征

刘名定1, 2, 3, 4,蔡永超2, 3,李洁2, 3,刘青2, 3,张健2, 3,吕丽娜2, 3,苏宏飞1, 4   

  1. 1. 广西大学资源环境与材料学院, 广西 南宁 530004;

    2. 热带海洋环境与岛礁生态全国重点实验室, 中国科学院南海海洋研究所, 广东 广州 510301;

    3. 海南三亚海洋生态系统国家野外科学观测研究站, 中国科学院海南热带海洋生物实验站, 海南 三亚 572000;

    4. 广西大学海洋学院, 广西南海珊瑚礁研究重点实验室, 广西 南宁 530004



  • 收稿日期:2025-10-29 修回日期:2025-12-01 接受日期:2025-12-09
  • 通讯作者: 苏宏飞
  • 基金资助:

    国家自然科学基金项目(U23A2036); 广西科技基地和人才专项(桂科AD25069075); 热带海洋环境与岛礁生态全国重点实验室自主项目(SKLTO2025PT002)

Isolation, identification and genomic characteristics of a coral-derived Benzophenone-3-degrading Sphingopyxis terrae SCSIO 90395

LIU Mingding1, 2, 3, 4, CAI Yongchao2, 3, LI Jie2, 3, LIU Qing2, 3, ZHANG Jian2, 3, LYU Lina2, 3, SU Hongfei1, 4   

  1. 1. School of Resources, Environment and Materials, Guangxi University, Nanning 530004, China;

    2. State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China;

    3. Sanya National Marine Ecosystem Research Station, Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, Sanya 572000, China;

    4. School of Marine Sciences, Guangxi University, Guangxi Laboratory on the Study of Coral Reefs in the South China Sea, Nanning 530004, China



  • Received:2025-10-29 Revised:2025-12-01 Accepted:2025-12-09
  • Supported by:

    National Natural Science Foundation of China (U23A2036); Guangxi Science and Technology Program (GuiKe AD25069075); The program of State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences (SKLTO2025PT002)

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摘要： 二苯甲酮-3(2-Hydroxy-4-methoxybenzophenone, BP-3)是一种广泛添加于防晒霜和个人护理产品中的有机紫外线吸收剂, 作为海洋新兴污染物, 已被证实在珊瑚礁生态系统中存在富集风险并对生态安全构成潜在威胁。为探究BP-3在珊瑚礁生态系统中的生物去除途径, 本研究从南海丛生盔型珊瑚(Galaxea fascicularis)组织样品中富集分离获得一株具备BP-3降解功能的海洋细菌Sphingopyxis terrae SCSIO 90395, 并分析其降解活性和潜在代谢机制。结果表明：在以BP-3为唯一碳源的条件下, 该菌株在48h内对BP-3的降解率达30%。S. terrae SCSIO 90395基因组大小为3,892,925bp, GC含量为64.81%, 共预测到3732个编码基因, 富含多个与芳香族化合物代谢和细胞色素P450相关的功能基因。分子对接分析结果进一步表明, 由gene0737编码的细胞色素P450酶与BP-3之间具有较高的结合亲和力, 具备启动BP-3生物降解的潜力。研究结果为发展基于珊瑚原位功能微生物的BP-3污染生物修复提供了新的菌种资源与理论支撑。

关键词: 珊瑚共生菌, 二苯甲酮-3, 微生物降解, 基因组分析

Abstract: Benzophenone-3 (2-Hydroxy-4-methoxybenzophenone, BP-3) is an organic ultraviolet (UV) filter widely used in sunscreens and personal care products. As an emerging marine contaminant, BP-3 bioaccumulates in coral reef ecosystems, posing possible ecological risks. To explore its biodegradation pathways, a BP-3 degrading bacteria, Sphingopyxis terrae SCSIO 90395, was isolated from the coral Galaxea fascicularis collected in the South China Sea. The strain degraded approximately 30% of BP-3 within 48 hours when provided as the sole carbon source. Genome analysis (3.89 Mb, 64.81%) revealed 3732 putative protein-coding sequences, including those associated with aromatic compound metabolism and cytochrome P450 enzymes. Molecular docking suggested that a cytochrome P450 enzyme encoded by gene0737 has high binding affinity to BP-3, implying a potential role in its biodegradation. These findings provided a novel microbial resource and establish a theoretical foundation for developing coral-associated microbial strategies for BP-3 bioremediation.

Key words: coral-associated bacteria, Benzophenone-3, microbial degradation, genome analysis