珊瑚来源潜在降解对甲氧基肉桂酸辛酯的细菌物种多样性

doi:10.11978/2024059

热带海洋学报 ›› 2025, Vol. 44 ›› Issue (1): 133-145.doi: 10.11978/2024059CSTR: 32234.14.2024059

珊瑚来源潜在降解对甲氧基肉桂酸辛酯的细菌物种多样性

黄沁愉¹^,²(), 吕丽娜², 李洁², 琚慧敏², 苏宏飞¹()

1.广西大学海洋学院, 广西南宁 530004
2.中国科学院热带海洋生物资源与生态重点实验室, 中国科学院南海海洋研究所, 广东广州 510301

收稿日期:2024-03-18 修回日期:2024-04-12 出版日期:2025-01-10 发布日期:2025-02-10
通讯作者: 苏宏飞
作者简介:
黄沁愉(1999—), 女, 广东省广州市人, 硕士研究生, 从事海洋珊瑚微生物相关研究。email: hyhqy172@sina.com
基金资助:
国家自然科学基金项目(U23A2036)

Microbial diversity of potential EHMC-degrading bacteria from coral

HUANG Qinyu¹^,²(), LYU Lina², LI Jie², JU Huimin², SU Hongfei¹()

1. School of Marine Sciences, Guangxi University, Nanning 530004, China
2. Key Laboratory of Tropical Marine Bio-resources and Ecology, Chinese Academy of Sciences, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received:2024-03-18 Revised:2024-04-12 Online:2025-01-10 Published:2025-02-10
Contact: SU Hongfei
Supported by:
National Natural Science Foundation of China(U23A2036)

摘要/Abstract

摘要：

对甲氧基肉桂酸辛酯(2-ethylhexyl-4-methoxycinnamic acid, EHMC)是国内防晒产品中最常见的有机紫外吸收剂成分, 随人类活动汇入海洋并在珊瑚组织内积累, 加速珊瑚白化。微生物降解法是去除珊瑚EHMC污染的有效手段之一, 然而目前珊瑚的EHMC降解细菌资源及EHMC对珊瑚微生物群落的影响仍知之甚少。本文选择三亚鹿回头近岸海域珊瑚为研究对象, 开展EHMC降解菌多样性研究。首先, 以EHMC为唯一碳源对珊瑚组织样品进行多次富集, 获得EHMC的耐受菌群, 利用高通量测序技术, 分析富集菌群组成和多样性; 同时通过细菌的分离鉴定和功能验证, 筛选出潜在的EHMC降解菌株。16S rRNA基因高通量测序结果显示, 在EHMC胁迫下, 滨珊瑚(Porites lutea)、角蜂巢珊瑚(Favosites sp.)和鹿角珊瑚(Acropora sp.)三个样品的细菌群落多样性下降。从属水平上看, 壤霉菌属(Agromyces)、副球菌属(Paracoccus)、Pelagibaca属和赤杆菌属(Erythrobacter)等细菌在原始珊瑚中占比极低, 经EHMC多次富集后成为优势菌属。通过纯化培养, EHMC富集样品共分离出60株可培养细菌。16S rRNA基因鉴定结果显示, 60株细菌分别归属于3个门、3个纲、6个目、8个科、13个属和17个种。可培细菌的优势菌属主要为芽孢杆菌属(Bacillus), 其次是红球菌属(Rhodococcus), 副球菌属, 微小杆菌属(Exiguobacterium)和尖球菌属(Acuticoccus)。经对单个纯培养菌株的活性筛选, 最终筛选出8株潜在EHMC降解菌株, 分别归属于芽孢杆菌属、短波单胞菌属(Brevundimonas)、副球菌属、尖球菌属、Phycicoccus属、红球菌属和分枝杆菌属(Mycolicibacterium)。前人研究表明, 红球菌属、芽孢杆菌属和副球菌属等细菌与芳香族化合物的生物降解相关。本研究分析了EHMC污染对珊瑚细菌群落的影响, 并分离出潜在的EHMC降解细菌, 为珊瑚抵御有机紫外吸收剂污染提供有用信息, 也为后续构建珊瑚益生微生物组减轻珊瑚礁有机污染积累了宝贵的菌种资源。

关键词: 珊瑚, 对甲氧基肉桂酸辛酯, 菌群多样性, 微生物降解

Abstract:

2-ethylhexyl-4-methoxycinnamic acid (EHMC) is an organic UV filter that is widely used in domestic sunscreen products. It is released into the ocean via human activities and accumulated in coral tissues, hastening coral bleaching. Biodegradation is an effective method for removing EHMC from coral, but little is known about its biodegradation in coral and its impact on coral microorganisms. To study the diversity of EHMC biodegradation in coral, we collected coral samples from Luhuitou, Sanya. These samples were enriched multiple times with EHMC as the sole carbon source to obtain EHMC-tolerance bacteria. High-throughput sequencing technology was used to analyze the composition and diversity of bacteria communities from enrichment samples. Besides, potential EHMC-degrading strains were isolated and identified from the enrichment through activity tests. High-throughput 16S rRNA gene sequencing revealed that the microbial community diversity in coral samples of Porites lutea, Favosites sp. and Acropora sp. was reduced under high concentration of EHMC. In contrast to their low abundance in the original coral samples, Agromyces, Paracoccus, Pelagibaca and Erythrobacter were the dominant genera after multiple enrichment with EHMC. Furthermore, a total of 60 bacterial strains were isolated from the EHMC-enriched samples. Based on 16S rRNA gene identification, these strains belonged to three phyla, three classes, six orders, eight families, thirteen genera and seventeen species. The main dominant genera among the cultivable bacteria were Bacillus, followed by Rhodococcus, Paracoccus, Exiguobacterium and Acuticoccus. Moreover, eight potential EHMC-degrading strains were selected through activity tests, belonging to Bacillus, Brevundimonas, Paracoccus, Acuticoccus, Phycicoccus, Rhodococcus and Mycolicibacterium, respectively. Previous studies have shown that Rhodococcus, Bacillus and Paracoccus were correlated with the biodegradation of aromatic compounds. This study illustrates the impact of EHMC on coral microbial communities and isolates potential EHMC-degrading bacteria, providing valuable information for coral resilience against organic UV filters. Additionally, it accumulates valuable bacterial resources for eliminating organic contaminants in coral by the subsequent construction of coral probiotic consortium.

Key words: coral, 2-ethylhexyl-4-methoxycinnamic acid, microbial diversity, microbial degradation

中图分类号:

P745

黄沁愉, 吕丽娜, 李洁, 琚慧敏, 苏宏飞. 珊瑚来源潜在降解对甲氧基肉桂酸辛酯的细菌物种多样性[J]. 热带海洋学报, 2025, 44(1): 133-145.

HUANG Qinyu, LYU Lina, LI Jie, JU Huimin, SU Hongfei. Microbial diversity of potential EHMC-degrading bacteria from coral[J]. Journal of Tropical Oceanography, 2025, 44(1): 133-145.

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珊瑚属名	珊瑚种名	拉丁名
蔷薇珊瑚属	截顶蔷薇珊瑚	Montipora truncata
蔷薇珊瑚属	蔷薇珊瑚	Montipora sp.
盘星珊瑚属	盘星珊瑚	Dipsastraea sp.
牡丹珊瑚属	厚板牡丹珊瑚	Pavona duerdeni
牡丹珊瑚属	十字牡丹珊瑚	Pavona decussata
鹿角珊瑚科属	巨锥鹿角珊瑚	Acropora monticulosa
	多孔鹿角珊瑚	Acropora millepora
	风信子鹿角珊瑚	Acropora hyacinthus
	鹿角珊瑚	Acropora sp.
盔形珊瑚属	从生盔珊瑚	Galaxea fascicularis
角孔珊瑚属	角孔珊瑚	Goniopora sp.
角蜂巢珊瑚属	角蜂巢珊瑚	Favosites sp.
滨珊瑚属	澄黄滨珊瑚	Porites lutea
杯形珊瑚属	鹿角杯形珊瑚	Pocillopora damicornis

珊瑚物种	样品编号	ACE指数	Chao1指数	Simpson 指数	Shannon 指数
滨珊瑚	Coral-PL	1888	1423	0.84	3.88
滨珊瑚	EHMC-PL	75	95	0.53	1.34
角蜂巢珊瑚	Coral-FA	1040	1052	0.90	5.45
角蜂巢珊瑚	EHMC-FA	84	69	0.43	1.53
鹿角珊瑚	Coral-AC	808	837	0.93	5.92
鹿角珊瑚	EHMC-AC	82	81	0.67	1.98

菌株来源	菌株编号	种名	相似度/%
杯形珊瑚	SCSIO 19749	Bacillus altitudinis 41KF2b	100.00
角蜂巢珊瑚	SCSIO 19792	Brevundimonas diminuta ATCC 11568	99.77
角蜂巢珊瑚	SCSIO 19789	Paracoccus rhizosphaerae CC-CCM15-8	99.17
鹿角珊瑚	SCSIO 19765	Acuticoccus sediminis PTG4-2	100.00
鹿角珊瑚	SCSIO 19799	Phycicoccus jejuensis NRRL B-24460	99.13
鹿角珊瑚	SCSIO 19755	Rhodococcus hoagii DSM 20295	99.42
牡丹珊瑚	SCSIO 19779	Mycolicibacterium poriferae ATCC 35087	99.86
滨珊瑚	SCSIO 19762	Paracoccus aerius 011410	97.59

珊瑚来源潜在降解对甲氧基肉桂酸辛酯的细菌物种多样性

Microbial diversity of potential EHMC-degrading bacteria from coral

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