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热带海洋学报

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热胁迫重塑虫黄藻Symbiodinium sp. CCMP 2433和细菌SCSIO 12696的相互作用

廉丹阳1,2,3, 蔡永超1,3,4, 琚慧敏5, 李洁1,3,4   

  1. 1. 热带海洋生物资源与生态实验室, 热带海洋环境与岛礁生态全国重点实验室, 中国科学院南海海洋研究所, 广东 广州 510301;

    2. 中国科学院大学, 北京 100049;

    3. 海南三亚海洋生态系统国家野外科学观测研究站, 中国科学院海南热带海洋生物实验站, 海南 三亚 572000;

    4. 海南省热带海洋生物技术重点实验室, 三亚海洋生态环境与工程研究院, 海南 三亚 572000;

    5. 江苏省海洋地质调查院, 江苏省地质局, 江苏 南京 210007



  • 收稿日期:2026-03-17 修回日期:2026-04-21 接受日期:2026-04-28
  • 通讯作者: 李洁
  • 基金资助:

    海南省自然科学基金(426ZD1018); 国家自然科学基金（U23A2036）

Heat Stress Remodels the Interaction Between Symbiodinium sp. CCMP 2433 and Bacterium SCSIO 12696

LIAN Danyang1, 2, 3, CAI Yongchao1, 3, 4, JU Huimin5, LI Jie1, 3, 4   

  1. 1. Laboratory of Tropical Marine Bio-Resources and Ecology, State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China;

    2. University of Chinese Academy of Sciences, Beijing 100049, China;

    3. Sanya National Marine Ecosystem Research Station, Tropical Marine Biological Research Station in Hainan,

    Chinese Academy of Sciences, Sanya 572000, China;

    4. Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya Institute of Ocean Eco-Environmental Engineering, Sanya 572000, China;

    5. Marine Geological Survey of Jiangsu Province, Jiangsu Geological Bureau, Nanjing 210007, China



  • Received:2026-03-17 Revised:2026-04-21 Accepted:2026-04-28
  • Supported by:

    Hainan Provincial Natural Science Foundation of China((426ZD1018); National Natural Science Foundation of China (U23A2036)

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摘要： 珊瑚共生体的健康与其内部微生物互作密切相关, 其中虫黄藻(Symbiodiniaceae)与细菌之间的互作对响应环境胁迫具有重要作用, 但其在热胁迫下的动态变化尚不明确。本研究以分离于鹿角杯形珊瑚组织(Pocillopora damicornis)的虫黄藻Symbiodinium sp. CCMP 2433和一株具有多种维生素合成能力的细菌SCSIO 12696(“Corallivirga polyvitaminum”)为材料，构建体外藻-菌共培养体系, 系统比较了25℃(对照)与32℃(热胁迫)培养条件下虫黄藻的生理指标、细菌的附着行为以及藻菌互作关系的动态变化。32℃(热胁迫)显著抑制了Symbiodinium sp. CCMP 2433的生长(细胞密度下降)、最大光化学效率(Fv/Fm降低)及光合色素含量(叶绿素a含量减少)。同时, 热胁迫显著促进了细菌SCSIO 12696在虫黄藻表面的附着, 改变了藻-菌共培养体系中的藻菌丰度比例, 使得Symbiodinium/SCSIO 12696细胞丰度比值下降。显微观察进一步发现, 热胁迫增强了细菌对虫黄藻细胞的附着, 共培养第5天观察到菌株SCSIO 12696可侵入藻细胞内部。结果表明, 温度对Symbiodinium-SCSIO 12696互作具有调控作用, 32℃(热胁迫)抑制虫黄藻的生长和光合生理并同时促进细菌SCSIO 12696的附着与增殖。这一发现为理解虫黄藻-细菌相互作用提供了实验证据, 有助于理解珊瑚共生体在热胁迫下的响应与适应性。

关键词: 热胁迫, 藻-菌互作, 共培养, 珊瑚共生体, 光合生理

Abstract: The health of coral holobiont is closely linked to their internal microbial interactions. In particular the interactions between Symbiodiniaceae and bacteria are crucial for responding to environmental stress. However, how these interactions are regulated under heat stress remains unclear. In this study, we used that were both isolated from the tissue of the coral Pocillopora damicornis: Symbiodinium sp. CCMP 2433 and a vitamin-producing bacterium SCSIO 12696 (“Corallivirga polyvitaminum”). This study systematically compared the dynamics of Symbiodinium physiology, bacterial (SCSIO 12696) attachment behavior, and their interactions under control (25°C) and heat stress (32°C) conditions. Heat stress significantly inhibited the growth (cell density), maximum photochemical efficiency (Fv/Fm), photosynthetic pigment content (chlorophyll a) of Symbiodinium sp. CCMP 2433. At the same time, heat stress significantly enhanced the attachment of the bacterium SCSIO 12696 to the Symbiodinium cell surface and altered the ratio in the co-culture system and resulting in a decreased Symbiodinium/SCSIO 12696 cell abundance ratio. Microscopic observations further confirmed that heat stress enhanced the attachment of the bacterial to Symbiodinium cells, and SCSIO 12696 was observed entering the Symbiodinium cells by the fifth day of co-culture. These results indicated that temperature plays a regulatory role in the Symbiodinium-SCSIO 12696 interaction. Under heat stress (32°C), the growth and photosynthetic physiology of Symbiodinium were inhibited, while the attachment and proliferation of bacterium SCSIO 12696 were promoted. This study provides experimental evidence for understanding the interaction between Symbiodiniaceae and bacteria, and contributes to the understanding of the response and adaptability of the coral holobiont under heat stress.

Key words: heat stress, Symbiodiniaceae-bacteria interaction, co-culture, coral holobiont, photosynthetic physiology