糙刺参饵料Amphora sp. 的营养价值分析及其 生物学特点的初步观察

doi:10.11978/j.issn.1009-5470.2013.03.009

热带海洋学报 ›› 2013, Vol. 32 ›› Issue (3): 59-64.doi: 10.11978/j.issn.1009-5470.2013.03.009cstr: 32234.14.j.issn.1009-5470.2013.03.009

糙刺参饵料Amphora sp. 的营养价值分析及其生物学特点的初步观察

周一春^{1, 2}, 彭鹏飞^{1, 2}, 胡超群¹, 钟鸣^{1, 2}, 陈言峰¹, 赵哲¹, 夏建军¹

1. 中国科学院南海海洋研究所, 中国科学院海洋生物资源可持续利用重点实验室, 广东省应用海洋生物学重点实验室, 广东广州 510301; 2. 中国科学院大学, 北京 100049

收稿日期:2012-01-17 修回日期:2012-05-21 出版日期:2013-08-27 发布日期:2013-08-28
通讯作者: 国家支撑计划项目(2009BAB44B02)
作者简介:周一春(1987—), 海南省海口市, 女, 硕士研究生, 从事海参饵料微藻培养的研究。E-mail: jiaxinhainan@163.com

Analysis of nutritional value and observation of biological characteristics in microalga Amphora sp. as food for sea cucumber Stichopus horrens

ZHOU Yi-chun^{1, 2}, PENG Peng-fei^{1, 2}, HU Chao-qun¹, ZHONG Ming^{1, 2}, CHEN Yan-feng¹, ZHAO Zhe¹, XIA Jian-jun¹

1. South China Sea Institute of Oceanology, Graduate University of Chinese Academy of sciences, Guangzhou 510301, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-01-17 Revised:2012-05-21 Online:2013-08-27 Published:2013-08-28
Contact: 胡超群, 男, 研究员。E-mail: cqhu@scsio.ac.cn

摘要/Abstract

摘要：

通过光学显微镜和电子显微镜技术观察分析糙刺参饵料双眉藻的生物学特点(包括形态、大小和附着能力), 并检测其营养成分, 结合该藻的生长速率和生长特性, 综合探讨其做为热带海参饵料的价值, 进一步为热带海参人工配合饲料提供理论参考。试验结果表明, Amphora sp. 细胞长为16—20μm, 宽为9—14μm, 附着能力较强, 比生长速率(SGR)为0.42d^-1, 生长较快, 经养殖实践得知该藻为糙刺参幼体阶段喜食的饵料微藻。营养学分析表明, Amphora sp. 细胞中含碳水化合物30.80%, 灰分27.70%, 蛋白质26.33%, 脂肪6.40%。Amphora sp. 的必需氨基酸和不饱和脂肪酸的含量很高, 分别为44.96%和54.471%, 其中, 多不饱和脂肪酸(PUFA)为26.661%, 二十碳五烯酸(EPA)和花生四烯酸(AA)分别占5.622%和3.267%, 亚油酸和亚麻酸的含量达2.112%。综合分析可得出: Amphora sp. 细胞大小适中、附着能力强、活动能力弱、繁殖速度较快、营养成分含量较高且比例较合理等特点, 是糙刺参幼参较佳的底栖硅藻饵料。

关键词: Amphora sp., 形态, 营养成分, 饵料价值

Abstract:

Amphorasp., a type of microalgae, has been used as feed of Stichopus horrensduring its larval stage in feeding experiment. In the study, to better understand the value of Amphorasp. as feed of tropical sea cucumber, we characterized the biological features using light and scanning electron microscopy, such as size and adhesion ability, and also measured the nutritional components and growth characteristics. Our results show that size of Amphorasp. is 16-20 μm × 9-14 μm. The specific growth rate (SGR) is 0.42 per day and it shows strong adhesion ability. Analysis of nutritional components indicates that contents of carbohydrates, ash, protein, and fat are respectively 30.80%, 27.70%, 26.33%, and 6.40% in Amphorasp. cell. Furthermore, contents of essential amino-acid (EAA) and unsaturated fatty acid (UFA) are very high in Amphorasp. cell, at 44.96% and 54.471%, respectively. Among them, the percentage of polyunsaturated fatty acid (PUFA) is 22.661%, eicosapentaenoic acid (EPA) and arachidonic acid (AA) are 5.622% and 3.26%, respectively, while linoleic acid and linolenic acid is only 2.112%. Overall, it can be concluded that Amphorasp. has the excellent biological characteristics, including moderate cell size, strong adhesion ability, weak movement, as well as reasonable nutrition content, and should be a good choice for feeding tropical sea cucumber at its larval stage. The data in the study also provide theoretical basis for developing artificial feeds for tropical sea cucumber.

Key words: Amphora sp., morphology, nutrients composition, dietary value

中图分类号:

S816.15

周一春, 彭鹏飞, 胡超群, 钟鸣, 陈言峰, 赵哲, 夏建军. 糙刺参饵料Amphora sp. 的营养价值分析及其生物学特点的初步观察[J]. 热带海洋学报, 2013, 32(3): 59-64.

ZHOU Yi-chun, PENG Peng-fei, HU Chao-qun, ZHONG Ming, CHEN Yan-feng, ZHAO Zhe, XIA Jian-jun. Analysis of nutritional value and observation of biological characteristics in microalga Amphora sp. as food for sea cucumber Stichopus horrens[J]. Journal of Tropical Oceanography, 2013, 32(3): 59-64.

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糙刺参饵料Amphora sp. 的营养价值分析及其生物学特点的初步观察

Analysis of nutritional value and observation of biological characteristics in microalga Amphora sp. as food for sea cucumber Stichopus horrens

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糙刺参饵料Amphora sp. 的营养价值分析及其 生物学特点的初步观察

Analysis of nutritional value and observation of biological characteristics in microalga Amphora sp. as food for sea cucumber Stichopus horrens

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糙刺参饵料Amphora sp. 的营养价值分析及其生物学特点的初步观察