热带海洋学报 ›› 2013, Vol. 32 ›› Issue (6): 88-95.doi: 10.11978/j.issn.1009-5470.2013.06.013cstr: 32234.14.j.issn.1009-5470.2013.06.013

• 海洋生物学 • 上一篇    下一篇

诸氏鲻虾虎鱼染色体组型分析

陈小曲, 黄韧, 李建军   

  1. 广东省实验动物监测所, 广东省实验动物重点实验室, 广东 广州 510663
  • 收稿日期:2011-10-14 修回日期:2013-03-30 出版日期:2013-12-20 发布日期:2014-01-21
  • 通讯作者: 李建军。Email: lijianjun1125@126.com
  • 作者简介:陈小曲(1986—), 女, 广东省遂溪市人, 从事水产养殖工作。Email: 278425411@qq.com
  • 基金资助:

    广东省科技计划项目(2007B080501004、2009B060300011、2010A040209005、2010B090301027)

Study on the karyotype of Mugilogobius chulae

CHEN Xiao-qu, HUANG Ren, LI Jian-jun   

  1. Guangdong Laboratory Animals Mornitoring Institute, Key Laboratory of Guangdong Laboratory Animals, Guangzhou 510663, China
  • Received:2011-10-14 Revised:2013-03-30 Online:2013-12-20 Published:2014-01-21

摘要:

通过研究诸氏鲻虾虎鱼(Mugilogobius chulae)的染色体, 分析了该鱼的细胞遗传学特征。取该鱼胚胎细胞, 经秋水仙素浸泡、低渗处理、卡诺氏固定液固定后, 用常规空气干燥法制作染色体标本, 吉姆萨(Giemsa)染色后对获得的中期分裂相进行核型分析。研究结果表明, 诸氏鲻虾虎鱼的染色体数目(2n)为44, 核型为10m+10sm+4st+20t, 染色体总臂数(NF)为64, 未发现多倍体现象, 也未发现异型性染色体和随体。

关键词: 诸氏鲻虾虎鱼, 染色体, 组型

Abstract:

In this study, the karyotype of Mugilogobius chulae was firstly reported. The examined materials are metaphase of the early embryo cells prepared by the colchicine-hypotonic-air drying technique. The results showed that M. chulae has 2n=44 diploid chromosomes and the karyotype of 2n=10m+10sm+4st+20t with its fundamental arm number (NF) =64. Neither the polyploidy chromosomes nor the sex chromosomes were found, and there was no visible evidence of the satellites.

Key words: Mugilogobius chulae, chromosome, karyotype

中图分类号: 

  • P735.541