激素诱导花鳗鲡精巢发育成熟期间性激素水平及相关细胞的超显微结构

doi:10.11978/j.issn.1009-5470.2010.06.130

热带海洋学报 ›› 2010, Vol. 29 ›› Issue (6): 130-136.doi: 10.11978/j.issn.1009-5470.2010.06.130cstr: 32234.14.j.issn.1009-5470.2010.06.130

激素诱导花鳗鲡精巢发育成熟期间性激素水平及相关细胞的超显微结构

齐鑫1, 黄海1, 2, 周雯伊1, 尹绍武1, 张勇2, 刘晓春2, 陈国华1, 林浩然1, 2

1. 海南大学海洋学院暨热带生物资源教育部重点实验室, 海南海口 570228; 2. 中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室, 广东广州 510275

收稿日期:2009-01-22 修回日期:2009-11-03 出版日期:2010-12-15 发布日期:2010-12-15
通讯作者: 林浩然。
作者简介:齐鑫(1983—), 男, 辽宁省沈阳市人, 硕士研究生, 主要从事鱼类生理学研究。E-mail: forever_qixin@yahoo.com.cn
基金资助:
国家自然基金面上项目(30770283); “十一五”国家科技支撑计划重点项目(2007BAD29B03); 海南省教育厅高等学校科学研
究资助项目(Hjkj2008-22); 广东省科技兴海(渔)项目(B200701A06)

Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata

QI Xin1, HUANG Hai1,2, ZHOU Wen-yi1, YIN Shao-wu1, ZHANG Yong2, LIU Xiao-chun2, CHEN Guo-hua1, LIN Hao-ran1,2

1. College of Ocean, Hainan University, Key Laboratory of Tropical Biology Resources, Ministry of Education, Haikou 570228, China; 2. Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou 510275, China

Received:2009-01-22 Revised:2009-11-03 Online:2010-12-15 Published:2010-12-15
Contact: 林浩然。
About author:齐鑫(1983—), 男, 辽宁省沈阳市人, 硕士研究生, 主要从事鱼类生理学研究。E-mail: forever_qixin@yahoo.com.cn
Supported by:
国家自然基金面上项目(30770283); “十一五”国家科技支撑计划重点项目(2007BAD29B03); 海南省教育厅高等学校科学研
究资助项目(Hjkj2008-22); 广东省科技兴海(渔)项目(B200701A06)

摘要/Abstract

摘要：

通过注射人类绒毛膜促性腺激素(HCG)诱导雄性花鳗鲡性腺发育成熟, 每7d注射一次(剂量为500U•(体重)kg?1), 共注射6次。检测此期间血清中促性腺激素(GtH)和性类固醇激素(T、11-KT、E2)的水平, 以及相关细胞超显微结构的变化。结果表明, 对照组雄性花鳗鲡性腺发育处于Ⅰ―Ⅱ期, GSI（Gonadosome Index）为0.17±0.06%, 血清中GtH、T、11-KT水平较低, 并且T浓度低于0.1ng(试剂盒测定范围为0.1―20ng•mL?1); 注射第3次后, 实验组鱼性腺发育处于Ⅲ期, GSI为7.0±0.03%, 血清中GtH、T、11-KT的水平均明显上升; 排精后的实验组鱼GSI为9.57±2.1%, GtH、T、11-KT的水平比注射第3次的实验组鱼有所下降, 但仍然高于对照组; 在整个催熟过程中, 血清中E2水平持续降低, 从对照组的2.36ng•mL?1下降到排精后的0.83ng•mL?1。超显微结构的观察证明, 与对照组相比排精后的实验组鱼脑垂体中GtH细胞的小颗粒减少, 内质网池扩大, 出现分泌并释放促性腺激素后留下的空泡。对照组鱼肝细胞富含糖原, 几乎没有脂肪泡的存在, 细胞核位于细胞中间, 排精后的实验组鱼肝细胞脂肪泡增多变大, 脂肪泡的形成导致细胞核偏位。

关键词: 花鳗鲡Anguilla marmorata;人工繁殖, 性类固醇激素, 促性腺激素, 精子, GtH细胞, 肝细胞

Abstract:

Serum levels of gonadotropin (GtH), testosterone (T), 11-ketotestosterone (11-KT), and estradiol-17β (E2) were assessed by radioimmunoassay during induction of sexual maturation of male marbled eel (Anguilla marmorata) by Human Chorionic Gonadotropin (HCG) treatment. Morphological alterations of the liver and GtH cell of Anguilla marmorata were investigated by means of light and electron microscopy. The testis Gonadosome Index (GSI) was 0.17±0.06% in the control group and their serum GtH and 11-KT levels were low, the T level was lower than 0.1 ng (the range of kit was 0.1―20ng•mL?1); Serum GtH, T, 11-KT levels increased rapidly in the operated group after the third injection of HCG, when the GSI of testis was 7.0±0.03%. After the spermiation, the GtH, T and 11-KT levels decreased a little, but still higher than the levels of the control group. Serum E2 levels decreased gradually as HCG treatment progressed, from 2.36 ng/ml of the control group to 0.83 ng/ml of the spermiation eels. In the hepat of the control group, abundant glycongen and little lipid droplets were obversived, the nuclear was in the middle of the cell. In contrast, in the hepat of the spermiation eel, the lipid droplets accu-mulated, which lead to the closing of nuclear to the cell membrane.

Key words: Anguilla marmorata, artifical reproduction, sex steroid hormone, gonadotropin, sperm, GtH cell, hepatic cells

齐鑫,黄海,周雯伊,尹绍武,张勇,刘晓春,陈国华,林浩然,. 激素诱导花鳗鲡精巢发育成熟期间性激素水平及相关细胞的超显微结构[J]. 热带海洋学报, 2010, 29(6): 130-136.

QI Xin,HUANG Hai,ZHOU Wen-yi,YIN Shao-wu,ZHANG Yong,LIU Xiao-chun,. Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata[J]. Journal of Tropical Oceanography, 2010, 29(6): 130-136.

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激素诱导花鳗鲡精巢发育成熟期间性激素水平及相关细胞的超显微结构

Variations in sex steroid hormone level and changes in ultrastructure of related cells during the artificial maturation of spermary of Anguilla marmorata

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