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热带海洋学报 ›› 2011, Vol. 30 ›› Issue (1): 107-112.doi: 10.11978/j.issn.1009-5470.2011.01.107cstr: 32234.14.j.issn.1009-5470.2011.01.107

• 海洋生物学 • 上一篇    下一篇

黑鱾精子发生过程中的超微结构变化

肖云朴1, 徐善良2, 孙敏3, 吕慧明3   

  1. 1. 平阳县海洋与渔业局, 浙江 平阳 325400; 2. 海洋生物工程浙江省重点实验室, 浙江 宁波 315211; 3. 应用海洋生物技术教育部重点实验室, 浙江 宁波 315211
  • 收稿日期:2009-01-16 修回日期:2010-05-14 出版日期:2011-03-17 发布日期:2011-03-16
  • 通讯作者: 徐善良。Tel: 13705749890, E-mail: xushanliang@nbu.edu.cn
  • 作者简介:萧云朴(1975—), 男, 浙江省平阳县人, 工程师, 从事水产养殖研究。E-mail: xiaoyunpu@21cn.com
  • 基金资助:

    浙江省农业重点科研项目(2007C22044)

Ultrastructural changes in Girella leonina during spermatogenesis

XIAO Yun-pu1, XU Shan-liang2, SUN Min3, LÜ Hui-ming3   

  1. 1. Ocean and Fisheries Bureau of Pingyang County, Pingyang 325400, China; 2. Key Laboratory of Marine Biotechnology of  Zhejiang, Ningbo 315211, China; 3.College of Life Sciences, Ningbo University, Ministry of Education, Ningbo 315211, China
  • Received:2009-01-16 Revised:2010-05-14 Online:2011-03-17 Published:2011-03-16
  • Contact: 徐善良。Tel: 13705749890, E-mail: xushanliang@nbu.edu.cn
  • About author:萧云朴(1975—), 男, 浙江省平阳县人, 工程师, 从事水产养殖研究。E-mail: xiaoyunpu@21cn.com
  • Supported by:

    浙江省农业重点科研项目(2007C22044)

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被引次数

7 | 4

摘要：

利用透射电镜对黑鱾Girella leonina精子发生过程中的各个阶段细胞以及精子的超微结构变化进行了观察。结果表明: 1)黑鱾的精子发生过程主要经历了初级精原细胞、次级精原细胞、初级精母细胞、次级精母细胞、精子细胞和精子6个阶段。由精子细胞形成成熟精子还需经过前、中、后期的一系列变态过程。2)各阶段生精细胞以初级精原细胞体积最大, 大小约为10.00?m×6.40?m, 核大小约为8.80?m×5.80?m, 而精子细胞体积最小, 大小约为3.20?m×2.60?m, 核约为2.80?m×2.50?m; 随着生精细胞不断分裂和成熟, 细胞的体积逐渐减小, 核的位置、核仁、线粒体等发生相应的变化, 核内染色质趋于浓缩, 电子密度增加, 染色程度也逐步加深。3)线粒体是各期细胞内的最主要细胞器之一, 在精子发生过程中, 它的数量逐渐减少, 至成熟精子形成时, 核内仅存4个线粒体, 但体积却不断增加, 内嵴变得长而明显。4)与大多数硬骨鱼类精子特点相似, 黑鱾的成熟精子不具顶体, 核后窝深, 精子的鞭毛细长, 两旁具侧鳍。尾部为典型的“9+2”型结构。

关键词: 黑鱾Girella leonina, 精子发生, 超微结构, 线粒体

Abstract:

The ultrastructural changes of the germ cells and sperm during spermatogenesis of Girella leonina were observed by transmission electronic microscope. The results are as follows. (1) The spermatogenesis of Girella leonina included six stages: primary spermatogonium, secondary spermatogonium, primary spermatocyte, secondary spermatocyte, spermatid, and sperm. These stages can be divided into three periods (before, during, and after) when the spermatid differentiated into sperm. (2) A primary spermatogonium was the largest of various stages of germ cells, and its cell was about 10.00μm × 6.40μm and the nuclear counted 8.80μm×5.80μm. The spermatid was the smallest, and its cell was about 3.20μm × 2.60μm and the nuclear counted 2.80μm×2.50μm. With spermatogenic cells continued to divide and mature, their size gradually reduced, the nuclear chromatin tended to concentrate, electron density increased, extent of staining deepened, and nuclear position, nucleolus, mi-tochondria also correspondingly changed. (3) Mitochondria were the main organelles in the germ cell, which gradually reduced the number in the process of spermatogenesis. At last, there were only four mitochondria in the mature sperm. On the contrary, the volume of mitochondria had kept increasing, and its cristae became clearer and longer. (4) As most kinds of teleostean, each sperm of Girella leonine consisted of a head without an acrosome, a midpiece, and a long tail that contains the lateral fins and a conventional “9 + 2” axoneme pattern.

Key words: Girella leonina, spermatogenesis, ultrastructural, mitochondria