检索
E-mail
RSS

作者投稿 主编审稿 专家审稿 远程编辑

热带海洋学报 ›› 2014, Vol. 33 ›› Issue (1): 69-73.doi: 10.11978/j.issn.1009-5470.2014.01.009cstr: 32234.14.j.issn.1009-5470.2014.01.009

• 海洋生物学 • 上一篇    下一篇

产酯酶海洋中度嗜盐菌Halomonas sp. LYG1-1的分离鉴定及其酶学性质

王昭凯, 易志伟, 刘洋, 产竹华, 曾润颖   

  1. 国家海洋局第三海洋研究所海洋生物遗传资源重点实验室, 福建 厦门361005
  • 收稿日期:2012-12-19 修回日期:2013-03-01 出版日期:2014-03-07 发布日期:2014-03-08
  • 通讯作者: 产竹华。E-mail: tiochan@yahoo.cn
  • 作者简介:王昭凯(1979~), 男, 山东省潍坊市人, 助理研究员, 硕士, 研究方向海洋微生物学。E-mail: wzkaicn@163.com
  • 基金资助:
    福建省自然科学基金资金项目(2012J01174)

Isolation, identification and characterization of an esterase-producing marine moderately halophilic strain Halomonas sp.LYG1-1

WANG Zhao-kai, YI Zhi-wei, LIU Yang, CHAN Zhu-hua, ZENG Run-ying   

  1. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China
  • Received:2012-12-19 Revised:2013-03-01 Online:2014-03-07 Published:2014-03-08

PDF (PC)

赞

摘要： 以从海南三亚近海海水中采集的样品为研究对象, 利用平板筛选法筛到一株产酯酶的中度嗜盐菌菌株LYG1-1。通过形态学、生理生化及16S rRNA 序列分析, 发现该菌株为革兰氏阴性菌, 其最适生长温度为30℃, 最适生长pH 为7.5, 最适NaCl浓度为10%。16S rRNA基因序列分析显示, 菌株LYG1-1与Halomonas salina 亲缘性最近, 16S rRNA基因序列相似性为99.72%。在含10% NaCl的0.05mol·L-1 Tris-HCl(pH 8.0)缓冲液中, 45℃条件下作用, 酶活性最高, 40℃处理24h仍保持83%的活性。金属离子浓度为5mmol·L-1时, Ca2+、Mg2+对菌株LYG1-1酶活具有明显的激活作用, Hg2+表现出强烈的抑制作用。

Abstract: Using plate screening method, a moderately halophilic strain LYG1-1 was isolated from seawater sample offshore of Sanya. According to physiological and biochemical characterizations, 16S rRNA sequences and phylogenetic analysis, cells were Gram-negative. It grew best at 30℃, pH 7.5 and with the presence of 10% NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that it belonged to the genus Halomonas, having the highest similarity (99.72%) with Halomonas salina. The maximum esterase activity was observed at 45℃, pH 8.0. The effects of various divalent cations were studied based on the activity of esterase from Halomonas sp. The concentrations of 5 mmol·L-1 Mg2+ and Ca2+ enhanced the esterase activity of LYG1-1, while the presence of Hg2+ strongly inhibited its activity.

中图分类号: 

  • Q178.53