基于线粒体控制区序列的花斑蛇鲻遗传多态性分析

doi:10.11978/2019115

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (4): 42-49.doi: 10.11978/2019115CSTR: 32234.14.2019115

基于线粒体控制区序列的花斑蛇鲻遗传多态性分析

李敏^1,^2,³(), 孔啸兰¹, 许友伟¹, 陈作志^1,^2,³()

1.中国水产科学研究院南海水产研究所, 农业农村部外海渔业开发重点实验室, 广东广州 510300
2.中国水产科学研究院南海水产研究所, 广东省渔业生态环境重点实验室, 广东广州 510300
3.南方海洋科学与工程广东省实验室(广州), 广东广州 511458

收稿日期:2018-11-13 修回日期:2020-01-11 出版日期:2020-07-20 发布日期:2020-07-27
通讯作者: 陈作志
作者简介:李敏(1984—), 男, 湖南长沙人, 副研究员, 博士, 从事海洋生物多样性保护研究。E-mail: limin@scsfri.ac.cn
基金资助:
广东省自然科学基金项目(2014A030310177);农业农村部财政专项(NFZX2018);广东省促进海洋经济发展专项资金(GDME-2018E004);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金(2019TS13)

Genetic polymorphism of the Brushtooth lizardfish Saurida undosquamis based on mitochondrial D-loop sequences

Min LI^1,^2,³(), Xiaolan KONG¹, Youwei XU¹, Zuozhi CHEN^1,^2,³()

1. Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
2. Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
3. Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China

Received:2018-11-13 Revised:2020-01-11 Online:2020-07-20 Published:2020-07-27
Contact: Zuozhi CHEN
Supported by:
Foundation item: Natural Science Foundation of Guangdong Province(2014A030310177);Financial Fund of the Ministry of Agriculture and Rural Affairs(NFZX2018);Special Fund for Promoting Marine Economic Development of Guangdong Province(GDME-2018E004);Central Public-interest Scientific Institution Basal Research Fund, South China Sea Fisheries Research Institute, CAFS(2019TS13)

摘要/Abstract

摘要：

花斑蛇鲻(Saurida undosquamis)是一种重要的底层经济鱼类, 本研究利用线粒体控制区(D-loop区)序列分析了中国近海分布的花斑蛇鲻的遗传结构和遗传多样性, 一共测定了6个地理群体129尾样本的D-loop区全序列。结果显示, 全长为921bp的序列包含71个多态性位点, 共检测到101个单倍型。花斑蛇鲻总体呈现很高的单倍型多样性(0.9873 ± 0.0048)和较低的核苷酸多样性(0.0132 ± 0.0067)的特征。基于邻接法构建的单倍型系统发育树的拓扑结构很浅, 没有形成分化明显的支系。单倍型在各个地理群体中的分布呈分散交叉状态, 表明地理群体间没有显著的遗传分化。6个群体的分子方差分析显示, 花斑蛇鲻绝大部分的遗传变异(99.87%)来源于群体内的个体之间, 而群体间的变异仅占0.13%。大部分地理群体之间的遗传分化指数(F_ST)均很小, 揭示了群体间的基因交流很频繁, 存在高度的遗传同质性。这些研究结果表明中国近海的花斑蛇鲻遗传多样性丰富, 没有明显的遗传分化, 是一个随机交配群, 可以作为同一个渔业单元来管理。

关键词: 蛇鲻, 底层鱼类, 渔业管理, 遗传结构, 遗传多样性

Abstract:

The Brushtooth lizardfish (Saurida undosquamis) is one of the economically important demersal fishes. The population genetic structure and genetic diversity of S. undosquamis from the coast of China were examined based on the complete control region (D-loop) sequences. A total of 129 individuals from six geographic populations were sequenced. Seventy-one polymorphic sites were detected, which defined 101 haplotypes. Results show S. undosquamis from the coast of China are characterized by quite high haplotype diversity (0.9873 ± 0.0048) and relative low nucleotide diversity (0.0132 ± 0.0067). Phylogenetic tree for haplotypes based on Neighbour-joining method shows shallow topology and reveals no significant divergent clades. Haplotypes from each geographic population were scattered throughout the NJ tree, showing no significant genetic differentiation between populations. Analyses of molecular variance suggest nearly all the genetic variation (99.87%) is attributed to variability within populations, while little variations (0.13%) are found between populations. Most of the pairwise F_STvalues between different populations are quite low, which implies a high rate of gene flow and genetic homogeneity between populations. The results demonstrate high genetic diversity and little genetic differentiation for S. undosquamis from the coast of China. They belong to the same population (panmixia), and a single-stock management regime could be supported in fishery management.

Key words: lizardfish, demersal fishes, fisheries management, genetic structure, genetic diversity

中图分类号:

P735

李敏, 孔啸兰, 许友伟, 陈作志. 基于线粒体控制区序列的花斑蛇鲻遗传多态性分析[J]. 热带海洋学报, 2020, 39(4): 42-49.

Min LI, Xiaolan KONG, Youwei XU, Zuozhi CHEN. Genetic polymorphism of the Brushtooth lizardfish Saurida undosquamis based on mitochondrial D-loop sequences[J]. Journal of Tropical Oceanography, 2020, 39(4): 42-49.

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地理群体(缩写)	采样点位置	样本量/个	单倍型数量/个	单倍型多样性(h±SD)	核苷酸多样性(π±SD)
防城港(FCG)	108°30'E, 21°00'N	21	19	0.9905 ± 0.0178	0.0110 ± 0.0058
三亚(SY)	109°46'E, 17°58'N	22	21	0.9957 ± 0.0153	0.0148 ± 0.0077
海口(HK)	111°18'E, 20°18'N	22	21	0.9957 ± 0.0153	0.0135 ± 0.0071
珠海(ZH)	114°05'E, 21°41'N	21	20	0.9952 ± 0.0165	0.0142 ± 0.0074
汕头(ST)	116°55'E, 23°00'N	22	19	0.9827 ± 0.0208	0.0129 ± 0.0068
泉州(QZ)	119°02'E, 24°36'N	21	21	1.0000 ± 0.0147	0.0128 ± 0.0068
总计	/	129	101	0.9873 ± 0.0048	0.0132 ± 0.0067

变异来源	自由度(无量纲)	变异百分比/%	分化指数(F_st)
群体间	5	0.13	/	/
群体内	123	99.87	/	/
所有样本	128	/	0.0013	0.3992

	FCG	SY	HK	ZH	ST	QZ
FCG		0.0773	0.0707	0.0202	0.1060	0.1605
SY	0.0269		0.9300	0.6829	0.8408	0.7405
HK	-0.0267	-0.0211		0.7952	0.9263	0.8341
ZH	0.0478	-0.0126	-0.0155		0.9421	0.2658
ST	-0.0506	-0.0176	-0.0204	-0.0238		0.3424
QZ	0.0164	-0.0155	-0.0178	0.0071	0.0017

	FCG	SY	HK	ZH	ST
SY	0.0146
HK	0.0351	0.7368
ZH	0.2855	0.2921	0.1818
ST	0.3906	0.7042	0.8730	0.6746
QZ	0.5270	0.3033	0.4864	0.3926	0.9453

基于线粒体控制区序列的花斑蛇鲻遗传多态性分析

Genetic polymorphism of the Brushtooth lizardfish Saurida undosquamis based on mitochondrial D-loop sequences

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