基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成*

doi:10.11978/2019105

热带海洋学报 ›› 2020, Vol. 39 ›› Issue (4): 61-69.doi: 10.11978/2019105CSTR: 32234.14.2019105

基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成^*

刘梦娜^1,^3,⁴(), 徐磊^3,⁴, 王雪辉^3,⁴, 刘玉⁵, 王淼娣^1,^3,⁴, 邱永松³, 朱江峰¹, 何映霖², 贝伟烈², 杜飞雁^3,⁴()

1.上海海洋大学海洋科学学院, 上海 201306
2.海南智渔可持续科技发展研究中心, 海南海口 570208
3.中国水产科学研究院南海水产研究所, 广东广州 510300
4.广东省渔业生态环境重点实验室, 广东广州 510300
5.中国科学院南海海洋研究所, 广东广州 510301

收稿日期:2019-10-29 修回日期:2020-01-19 出版日期:2020-07-20 发布日期:2020-07-27
通讯作者: 杜飞雁
作者简介:刘梦娜(1992—), 女, 河南省焦作市人, 在读硕士, 研究方向为海洋生物学。E-mail: monarliu@163.com
基金资助:
国家科技基础资源调查专项(2017FY201405);中国水产科学研究院基本科研业务费资助(2019HY-JC0203);广东省渔业生态环境重点实验室开放基金(FEEL-2019-9)

Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence

Mengna LIU^1,^3,⁴(), Lei XU^3,⁴, Xuehui WANG^3,⁴, Yu LIU⁵, Miaodi WANG^1,^3,⁴, Yongsong QIU³, Jiangfeng ZHU¹, Yinglin HE², Weilie BEI², Feiyan DU^3,⁴()

1. College of Marine Science, Shanghai Ocean University, Shanghai 201306, China
2. China Blue Sustainability Institute, Haikou 570208, China
3. South China Sea Fisheries Research Institute, China Academy of Fishery Sciences, Guangzhou 510300, China
4. Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China
5. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

Received:2019-10-29 Revised:2020-01-19 Online:2020-07-20 Published:2020-07-27
Contact: Feiyan DU
Supported by:
Foundation item: The Science and Technology Project of Basic Resources of China(2017FY201405);Basal Research Fund of Chinese Academy of Fishery Sciences(2019HY-JC0203);Open Foundation of Key Laboratory of Fishery Ecology Environment of Guangdong Province(FEEL-2019-9)

摘要/Abstract

摘要：

中国枪乌贼(Uroteuthis chinensis)和鸢乌贼(Sthenoteuthis oualaniensis)作为中国南海头足类的关键种, 对海洋生态系统的物质流动与能量循环具有重要影响, 其摄食生态的研究将对海洋食物网的构建具有重要意义。由于传统胃含物分析法难以准确鉴定糜状饵料生物的组成, 本研究利用DNA条码技术, 针对中国枪乌贼和鸢乌贼不可辨认的食物糜提取组织DNA, 选用线粒体基因细胞色素C氧化酶亚基I(Mitochondrial cytochrome oxidase subunit I, COI)作为分子标记, 获得的序列在Genbank中进行比对分析, 并使用GMYC(Generalized Mixed Yule Coalescent)模型进行物种界定和构建系统进化关系。结果成功鉴定出中国枪乌贼饵料物种有13种, 鸢乌贼饵料物种有8种, 共20种(其中1种为共有饵料)。比较发现, 汕头-台湾浅滩渔场的中国枪乌贼主要摄食鱼类、甲壳类和头足类, 而南海中部海域鸢乌贼则主要摄食鱼类和头足类。两物种均存在同类相食现象, 但鸢乌贼表现更明显。

关键词: 中国枪乌贼, 鸢乌贼, 摄食, COI基因, DNA条形码, 南海, 汕头-台湾浅滩渔场

Abstract:

Uroteuthis chinensis and Sthenoteuthis oualaniensis are key species of the cephalopods in the South China Sea, which have important effects on the material flow and energy circulation in the marine ecosystem. The study of feeding ecology of these two cephalopods will be great significant to the construction of marine food web. Because of the sharp tooth decay, the stomach contents are all scorpion-like, and it is difficult to accurately identify them by traditional morphological methods. And the effectiveness of molecular techniques in feeding analysis of these two species has not been fully validated. In this study, we identified the species (or genus) in the stomach contents of U. chinensis and S. oualaniensis based on the mitochondrial COI gene, and used the Generalized Mixed Yule Coalescent (GMYC) model for species definition and phylogenetic tree building. The results showed that of a total of 41 stomach contents sequences (28 from U. chinensis and 13 from S. oualaniensis), 30 (73%) were identified as species, and the rest were determined as genus (all are crustaceans). The diets of U. chinensis in Shantou-Taiwan Shoal fishing ground included fish, cephalopods, and crustaceans; and the diets of S. oualaniensis in the Central South China Sea were fish and cephalopods. Cannibalism occurred in both species, but was more pronounced in S. oualaniensis.

Key words: Uroteuthis chinensis, Sthenoteuthis oualaniensis, diet, COI, DNA barcoding, South China Sea, Shantou-Taiwan Shoal fishing ground

中图分类号:

P735

刘梦娜, 徐磊, 王雪辉, 刘玉, 王淼娣, 邱永松, 朱江峰, 何映霖, 贝伟烈, 杜飞雁. 基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成^*[J]. 热带海洋学报, 2020, 39(4): 61-69.

Mengna LIU, Lei XU, Xuehui WANG, Yu LIU, Miaodi WANG, Yongsong QIU, Jiangfeng ZHU, Yinglin HE, Weilie BEI, Feiyan DU. Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence[J]. Journal of Tropical Oceanography, 2020, 39(4): 61-69.

图/表 7

表1

表2

图1

表3

图2

表4

图3

参考文献 34

[1]	陈大纲, 张美昭, 2015. 中国海洋鱼类[M]. 青岛: 中国海洋大学出版社: 280-1883(in Chinese).
[2]	陈新军, 2004. 渔业资源与渔场学[M]. 北京: 海洋出版社: 51-52(in Chinese).
[3]	陈新军, 刘必林, 王尧耕, 2009. 世界头足类[M]. 北京: 海洋出版社: 312-313(in Chinese).
[4]	戴泉水, 卢振彬, 洪明进, 等, 2004. 台湾海峡南部海域游泳生物区系组成和渔业资源现状[J]. 中国水产科学, 11(4):360-366.
	DAI QUANSHUI, LU ZHENBIN, HONG MINGJIN, et al, 2004. Fauna composition of necton and fishery resources in the southern waters of Taiwan Strait[J]. Journal of Fishery Sciences of China, 11(4):360-366 (in Chinese with English abstract).
[5]	董正之, 1988. 中国动物志, 软体动物门, 头足纲[M]. 北京: 科学出版社: 88-95(in Chinese).
[6]	范江涛, 冯雪, 邱永松, 等, 2013. 南海鸢乌贼生物学研究进展[J]. 广东农业科学, 40(23):122-128.
	FAN JIANGTAO, FENG XUE, QIU YONGSONG, et al, 2013. Review on the biology of purpleback flying squid in South China Sea[J]. Guangdong Agricultural Science, 40(23):122-128 (in Chinese with English abstract).
[7]	龚金科, 1981. 闽南-台湾浅滩渔场台湾枪乌贼的洄游分布和生物学特性的探讨[J]. 福建水产科技, ( 2):15-26 (in Chinese).
[8]	龚玉艳, 詹凤娉, 杨玉滔, 等, 2016. 南海鸢乌贼摄食习性的初步研究[J]. 南方水产科学, 12(4):80-87.
	GONG YUYAN, ZHAN FENGPING, YANG YUTAO, et al, 2016. Feeding habits of Symplectoteuthis oualaniensis in the South China Sea[J]. South China Fisheries Science, 12(4):80-87 (in Chinese with English abstract).
[9]	龚玉艳, 孔啸兰, 杨玉滔, 等, 2018. 南海鸢乌贼微型群体的摄食习性研究[J]. 海洋渔, 40(4):394-403.
	GONG YUYAN, KONG XIAOLAN, YANG YUTAO, et al, 2018. Feeding habits of dwarf-form Sthenoteuthis oualaniensis population in the South China Sea[J]. Marine Fisheries, 40(4):394-403 (in Chinese with English abstract).
[10]	黄美珍, 2004. 台湾海峡及邻近海域4种头足类的食性和营养级研究[J]. 台湾海峡, 23(3):331-340.
	HUANG MEIZHEN, 2004. Study on feeding habits and nutrient level of four cephalopod species from Taiwan Strait and its adjacent areas[J]. Journal of Oceanography in Taiwan Strait, 23(3):331-340 (in Chinese with English abstract).
[11]	李斌, 陈国宝, 郭禹, 等, 2016. 南海中部海域渔业资源时空分布和资源量的水声学评估[J]. 南方水产科学, 12(4):28-37.
	LI BIN, CHEN GUOBAO, GUO YU, et al, 2016. Hydroacoustic assessment of spatial-temporal distribution and biomass of fishery resources in the central South China Sea[J]. South China Fisheries Science, 12(4):28-37 (in Chinese with English abstract).
[12]	唐启升, 1999. 海洋食物网与高营养层次营养动力学研究策略[J]. 海洋水产研究, 20(2):1-6.
	TANG QISHENG, 1999. Strategies of research on marine food web and trophodynamics between high trophic levels[J]. Marine Fisheries Research, 20(2):1-6 (in Chinese with English abstract).
[13]	颜云榕, 冯波, 卢伙胜, 等, 2012. 南沙群岛北部海域鸢乌贼(Sthenoteuthis oualaniensis)夏季渔业生物学研究[J]. 海洋与湖沼, 43(6):1177-1186.
	YAN YUNRONG, FENG BO, LU HUOSHENG, et al, 2012. Fishery biology of purpleback flying squid Sthenoteuthis oualaniensis in northern sea areas around Nansha islands in summer[J]. Oceanologia et Limnologia Sinica, 43(6):1177-1186 (in Chinese with English abstract).
[14]	张宇美, 颜云榕, 卢伙胜, 等, 2013. 西沙群岛海域鸢乌贼摄食与繁殖生物学初步研究[J]. 广东海洋大学学报, 33(3):56-64.
	ZHANG YUMEI, YAN YUNRONG, LU HUOSHENG, et al, 2013. Study on feeding and reproduction biology of purple flying squid, Sthenoteuthis oualaniensis in the western south china sea[J]. Journal of Guangdong Ocean University, 33(3):56-64 (in Chinese with English abstract).
[15]	张壮丽, 叶孙忠, 洪明进, 等, 2008. 闽南—台湾浅滩渔场中国枪乌贼生物学特性研究[J]. 福建水产, 3(1):1-5.
	ZHANG ZHUANGLI, YE SUNZHONG, HONG MINGJIN, et al, 2008. Biological characteristics of the Chinese squid (Loligo chinensis) in Minnan-Taiwan shallow fishing ground[J]. Journal of Fujian Fisheries, 3(1):1-5 (in Chinese with English abstract).
[16]	郑玉水, 杨甘霖, 曾建忠, 等. 台湾海峡枪乌贼资源探捕调查报告[J]. 福建水产. 1988: 8-16.
[17]	ALTSCHUL S F, MADDEN T L, SCHAFFER A A, et al, 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs[J]. Nucleic Acids Research, 25(17):3389-3402. pmid: 9254694
[18]	COSTA F O, DEWAARD J R, BOUTILLIER J, et al, 2007. Biological identifications through DNA barcodes: the case of the Crustacea[J]. Canadian Journal of Fisheries and Aquatic Sciences, 64(2):272-295.
[19]	DEAGLE B E, EVESON J P, JARMAN S N, 2006. Quantification of damage in DNA recovered from highly degraded samples--a case study on DNA in faeces[J]. Frontiers in Zoology, 3:11. pmid: 16911807
[20]	DRUMMOND A J, SUCHARD M A, XIE D, et al, 2012. Bayesian Phylogenetics with BEAUti and the BEAST 1.7[J]. Molecular Biology and Evolution, 29(8):1969-1973.
[21]	EZARD T, FUJISAWA T, BARRACLOUGH T G, 2009. splits: SPecies’ LImits by Threshold Statistics. R package version 1.0-14/r31. http://R-Forge.R-project.org/ projects/splits/.
[22]	FOLMER O, BLACK M, HOEH W, et al, 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol, 3(5):294-299. https://www.ncbi.nlm.nih.gov/pubmed/7881515 pmid: 7881515
[23]	FUJISAWA T, BARRACLOUGH T G, 2013. Delimiting species using single-locus data and the generalized mixed Yule coalescent approach: a revised method and evaluation on simulated data sets[J]. Systematic Biology, 62(5):707-724.
[24]	GELLER J, MEYER C, PARKER M, et al, 2013. Redesign of PCR primers for mitochondrial cytochrome c oxidase subunit I for marine invertebrates and application in all-taxa biotic surveys[J]. Molecular Ecology Resources, 13(5):851-861. https://www.ncbi.nlm.nih.gov/pubmed/23848937 pmid: 23848937
[25]	KING R A, READ D S, TRAUGOTT M, et al, 2008. INVITED REVIEW: Molecular analysis of predation: a review of best practice for DNA-based approaches[J]. Molecular Ecology, 17(4):947-963. https://www.ncbi.nlm.nih.gov/pubmed/18208490 pmid: 18208490
[26]	LERAY M, YANG J Y, MEYER C P, et al, 2013. A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents[J]. Frontiers in Zoology, 10(1):34.
[27]	MARKAIDA U, SOSA-NISHIZAKI O, 2003. Food and feeding habits of jumbo squid Dosidicus gigas (Cephalopoda: Ommastrephidae) from the Gulf of California, Mexico[J]. Journal of the Marine Biological Association of the United Kingdom, 83(3):507-522.
[28]	MEYER C P. 2003. Molecular systematics of cowries (Gastropoda: Cypraeidae) and diversification patterns in the tropics[J]. Biological Journal of the Linnean Society, 79(3):401-459.
[29]	PAGE R D M, 1996. TreeView: an application to display phylogenetic trees on personal computers[J]. Computer Applications in the Biosciences: CABIOS, 12(4):357-358. https://www.ncbi.nlm.nih.gov/pubmed/8902363 pmid: 8902363
[30]	POSADA D, CRANDALL K A, 2001. Selecting the best-fit model of nucleotide substitution[J]. Systematic Biology, 50(4):580-601. https://www.ncbi.nlm.nih.gov/pubmed/12116655 pmid: 12116655
[31]	SHCHETINNIKOV A S, 1992. Feeding spectrum of squid Sthenoteuthis oualaniensis (Oegopsida) in the eastern Pacific[J]. Journal of the Marine Biological Association of the United Kingdom, 72(4):849-860.
[32]	SYMONDSON W O C, 2002. Molecular identification of prey in predator diets[J]. Molecular Ecology, 11(4):627-641. https://www.ncbi.nlm.nih.gov/pubmed/11972753 pmid: 11972753
[33]	WARD R D, ZEMLAK T S, INNES B H, et al, 2005. DNA barcoding Australia's fish species[J]. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462):1847-1857.
[34]	XU LEI, WANG LIANGGEN, NING JIAJIA, et al, 2019. Diversity of marine planktonic ostracods in South China Sea: a DNA taxonomy approach[J]. Mitochondrial DNA Part A, 30(1):118-125.

	站位	东经	北纬
鸢乌贼	S3	111°37'50"	14°58'04"
	S9	110°35'06"	14°02'10"
	S16	117°28'28"	13°00'00"
	S18	110°30'35"	11°02'09"
	S19	111°28'51"	10°59'30"
	S20	113°51'30"	11°02'17"
	S21	114°32'36"	11°06'13"
	S26	110°28'22"	9°30'53"
中国枪乌贼	SQ	118°29'21"	23°05'46"

引物名称	序列(5′-3′)	参考文献
HCO2198(下游)	TAAACTTCAGGGTGACCAAAAAATCA	Folmer et al, 1994
dgHCO2198(下游)	TAAACTTCAGGGTGACCAAARAAYCA	Meyer, 2003
jgHCO2198(下游)	TAIACYTCIGGRTGICCRAARAAYCA	Geller et al, 2013
mlCOIintF(上游)	GGWACWGGWTGAACWGTWTAYCCYCC	Leray et al, 2013

最佳匹配物种	相似度	序列号	样品号
克氏副葉鰺 Alepes Swainson	99.90%	KY371115.1	N0709、N0711、N0752、N0759、N0767、N0771、N0772
蓝圆鲹 Decapterus maruadsi	100.00%	MK264594.1	N0718
金带细鲹 Selaroides leptolepis	99.64%	JX261038.1	N0740、N0742、N0779
三线鯻 Terapon puta	100.00%	KY372209.1	N0722
怀氏方头鲳 Cubiceps whiteleggii	100.00%	KY371367.1	N0743
黄泽小沙丁鱼 Sardinella lemuru	100.00%	MK264430.1	N0758
角突仿对Kishinouyepenaeopsis amicus	98.71%	KR349255.1	N0720、N0723
赤虾属 Metapenaeopsis sp.	87.05%	KR150460.1	N0753
刺铠虾属 Munida sp.	86.58%	AY350938.1	N0749、N0754、N0757、N0761、N0768、N0769、N0775
招潮蟹属 Uca sp.	84.40%	AB813678.1	N0735
团扇蟹属 Ozius sp.	87.45%	HM638053.1	N0770
梭子蟹属Portunus sp.	88.67%	JX398096.1	N0777
中国枪乌贼 Uroteuthis chinensis	100.00%	MG192385.1	N0736

最佳匹配物种	相似度	序列号	样品号
孔锯鳞鱼 Myripristis kuntee	99.53%	MK658063.1	S20-41-2
尾斑棘鳞鱼 Sargocentron caudimaculatum	100.00%	MF409503.1	S19-44-4
克氏副葉鰺 Alepes Swainson	100.00%	MK264421.1	S3-23-3
脂眼凹肩鲹 Selar crumenophthalmus	100.00%	MH638727.1	S26-14-2
鲣鱼 Katsuwonus pelamis	99.78%	MH638766.1	S19-44-3、S16-24-3
金焰灯笼魚 Myctophum aurolaternatum	100.00%	KJ555427.1	S20-28-3
鸢乌贼微型群 Sthenoteuthis oualaniensis	100.00%	MF411130.1	S18-1、S18-14、S19-31-2
鸢乌贼 Sthenoteuthis oualaniensis	100.00%	MF411105.1	S21-45-1、S3-25、S9-22

基于DNA条形码研究中国枪乌贼和鸢乌贼的食物种类组成^*

Study on food contents of Uroteuthis chinensis and Sthenoteuthis oualaniensis based on COI sequence

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 34

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	徐超, 龙丽娟, 李莎, 袁丽, 徐晓璐. 南海及其附属岛礁海洋科学考察历史资料系统整编3. 数据共享服务及应用[J]. 热带海洋学报, 2024, 43(5): 158-165.
[2]	徐超, 龙丽娟, 李莎, 何云开, 袁丽, 徐晓璐. 南海及其附属岛礁海洋科学考察历史资料系统整编1. 资料整编技术及应用[J]. 热带海洋学报, 2024, 43(5): 143-149.
[3]	徐超, 龙丽娟, 李莎, 徐晓璐, 袁丽. 南海及其附属岛礁海洋科学考察历史资料系统整编2. 数据治理技术与应用[J]. 热带海洋学报, 2024, 43(5): 150-157.
[4]	柳原, 柯志新, 李开枝, 谭烨辉, 梁竣策, 周伟华. 人类活动和沿岸流影响下的粤东近海浮游动物群落特征[J]. 热带海洋学报, 2024, 43(4): 98-111.
[5]	刘玓玓, 张喜洋, 孙富林, 王明壮, 谭飞, 施祺, 王冠, 杨红强. 南海海滩岩微生物群落结构和特定菌株对其成因机制的启示*[J]. 热带海洋学报, 2024, 43(4): 112-122.
[6]	胡思敏, 周天成, 张琛, 刘胜, 李涛, 黄晖. 悬浮物对三亚珊瑚礁区浮游动物群落结构及其摄食的影响[J]. 热带海洋学报, 2024, 43(3): 122-130.
[7]	江绿苗, 陈天然, 赵宽, 张婷, 许莉佳. 南海北部涠洲岛边缘珊瑚礁的生物侵蚀实验研究[J]. 热带海洋学报, 2024, 43(3): 155-165.
[8]	许莉佳, 廖芝衡, 陈辉, 王永智, 黄柏强, 林巧云, 甘健锋, 杨静. 南海北部珊瑚群落结构特征及其对海洋热浪事件的响应[J]. 热带海洋学报, 2024, 43(3): 58-71.
[9]	邱燕, 鞠东, 黄文凯, 王英民, 聂鑫. 南海中央海盆海底初始扩张时间的重新认定[J]. 热带海洋学报, 2024, 43(2): 154-165.
[10]	赵明辉, 袁野, 张佳政, 张翠梅, 高金尉, 王强, 孙珍, 程锦辉. 南海北部被动陆缘洋陆转换带张裂-破裂研究新进展[J]. 热带海洋学报, 2024, 43(2): 173-183.
[11]	黄谕, 王琳, 麦志茂, 李洁, 张偲. 南海热带岛礁生物土壤结皮中细菌的分离及其固砂特性初步研究[J]. 热带海洋学报, 2023, 42(6): 101-110.
[12]	王辰燕, 史敬文, 颜安南, 康亚茹, 王煜轩, 覃素丽, 韩民伟, 张瑞杰, 余克服. 有机磷酸酯在南海长棘海星中的生物富集特征及来源解析[J]. 热带海洋学报, 2023, 42(5): 30-37.
[13]	李牛, 邸鹏飞, 冯东, 陈多福. 冷泉渗漏对海洋沉积物氧化还原环境地球化学识别的影响——以南海东北部F站位活动冷泉为例^*[J]. 热带海洋学报, 2023, 42(5): 144-153.
[14]	张智晟, 谢玲玲, 李君益, 李强. 边缘海与开阔海中尺度涡生命周期演化规律对比分析: 以南海和黑潮延伸体为例[J]. 热带海洋学报, 2023, 42(4): 63-76.
[15]	杨磊, 温金辉, 王强, 罗希, 黄华明, 何云开, 陈举. 热带气旋影响吕宋海峡输运的研究进展与展望^*[J]. 热带海洋学报, 2023, 42(3): 40-51.